Plant germline development: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
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| Dokumenttyp: | Book/Monograph |
| Sprache: | Englisch |
| Veröffentlicht: |
New York, NY
Humana Press
[2017]
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| Schriftenreihe: | Methods in molecular biology
1669 |
| In: |
Methods in molecular biology (1669)
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis, Plant germline development, Inhaltsverzeichnis: http://digitool.hbz-nrw.de:1801/webclient/DeliveryManager?pid=7440955&custom_att_2=simple_viewer
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| Verfasserangaben: | edited by Anja Schmidt |
Inhaltsangabe:
- Introduction to male germline development Hannes Vogler, Ueli Grossniklaus, and Afif Hedhly
- Apomixis : engineering the ability to harness hybrid vigor in crop plants Joann A. Conner and Peggy Ozias-Akins
- 3D imaging of whole-mount ovules at cellular resolution to study female germline development in rice Ethel Mendocilla-Sato, Wenjing She, and Celia Baroux
- Live-cell imaging of F-actin dynamics during fertilization in Arabidopsis thaliana Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, and Tomokazu Kawashima
- Development and observation of mature megagametophyte cell-specific fluorescent markers Mark A. Chamberlin and Shai J. Lawit
- Analysis of fluorescent reporter activity in the male germline during pollen development by confocal microscopy David Twell and Lynette Brownfield
- In vivo ploidy determination of Arabidopsis thaliana male and female gametophytes Burcu Nur Keceli, Nico De Storme, and Danny Geelen
- Staining and clearing of arabidopsis reproductive tissue for imaging of fluorescent proteins Daniel Slane, Patrick Burgel, and Martin Bayer
- Live-cell imaging of auxin and cytokinin signaling in maize female gametophytes Antony M. Chettoor and Matthew M.S. Evans
- Imaging Ca2+ dynamics in wild-type and NADPH oxidase-deficient mutant pollen tubes with yellow cameleon and confocal laser scanning microscopy Christina Maria Franck, Jens Westermann, and Aurelien Boisson-Dernier
- Immunolocalization of AGPs and pectins in Quercus suber gametophytic structures Mario Luıs da Costa, Ana Lucia Lopes, Maria Isabel Amorim, and Sılvia Coimbra
- Optimization of cell spreading and image quality for the study of chromosomes in plant tissues Laksana Kantama, Erik Wijnker, and Hans de Jong
- Whole mount RNA-FISH on ovules and developing seeds Andrea Bleckmann and Thomas Dresselhaus
- Analysis of peroxisome biogenesis in pollen by confocal microscopy and transmission electron microscopy Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang
- Transmission electron microscopy (TEM) to study histology of pollen and pollen tubes Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang
- Isolation of Arabidopsis pollen, sperm cells, and vegetative nuclei by fluorescence-activated cell sorting (FACS) Mario R. Santos, Claudia Bispo, and Jorg D. Becker
- Isolation of rice sperm cells for transcriptional profiling Scott D. Russell, Daniel S. Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, and Xiaoping Gou
- Manual isolation of living cells from the Arabidopsis thaliana female gametophyte by micromanipulation Maria Englhart, Lucija Soljic, and Stefanie Sprunck
- Isolation and detection of small RNAs from pollen German Martinez
- Analysis of proteins enriched in rice gamete Takashi Okamoto
- Phosphoprotein enrichment from tobacco mature pollen crude protein extract Jan Fıla and David Honys Identification of cis-regulatory modules that function in the male germline of flowering plants
- RNA-seq data analysis : from raw data quality control to differential expression analysis Weihong Qi, Ralph Schlapbach, and Hubert Rehrauer
- RNA-seq data analysis protocol : combining in-house and publicly available data Marc W. Schmid
- Impedance flow cytometry as a tool to analyze microspore and pollen quality Iris Heidmann and Marco Di Berardino
- Agrobacterium-mediated Sorghum transformation Emily Wu and Zuo-Yu Zhao
- Use of the Cas9 orthologs from Streptococcus thermophilus and Staphylococcus aureus for non-homologous end-joining mediated site-specific mutagenesis in Arabidopsis thaliana Jeannette Steinert, Carla Schmidt, and Holger Puchta
- Transgenic reproductive cell ablation Shai J. Lawit and Mark A. Chamberlin
- In vivo reporters for protein half-life Pavel Reichman and Nico Dissmeyer
- Conditional modulation of biological processes by low-temperature degrons Nico Dissmeyer