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Multiple myeloma: methods and protocols

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This volume provides a thorough guide of the study of multiple myeloma (MM)-from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in this book cover topics, such as next-generation-...

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Bibliographic Details
Other Authors: Heuck, Christoph (Editor) , Weinhold, Niels (Editor)
Format: Book/Monograph
Language:English
Published: New York, NY Humana Press [2018]
Series:Methods in molecular biology 1792
In: Methods in molecular biology (1792)

Subjects:
Plasmozytom
Online Access:Verlag, Inhaltstext: http://deposit.dnb.de/cgi-bin/dokserv?id=7e821ff3c590483ab47f99e972403569&prov=M&dok_var=1&dok_ext=htm
Cover: https://swbplus.bsz-bw.de/bsz50759021xcov.jpg
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Author Notes:edited by Christoph Heuck, Niels Weinhold
Table of Contents:
  • ELDA qASO-PCR for high sensitivity detection of tumor cells in bone marrow and peripheral blood Stefanie Huhn
  • EuroFlow-based next-generation flow cytometry for detection of circulating tumor cells and minimal residual disease in multiple myeloma Leire Burgos and Bruno Paiva
  • Cytoplasmic immunoglobulin vs. DNA analysis by flow cytometry Daisy Alapat
  • Deep profiling of the immune system of multiple myeloma patients using cytometry by time-of-flight (CyTOF) Tina Smets, Frederik Stevenaert, Homer Adams III, and Greet Vanhoof
  • Fluorescence in situ hybridization (FISH) in multiple myeloma Erming Tian
  • Whole exome sequencing in multiple myeloma to identify somatic single nucleotide variants and key translocations involving immunoglobulin loci and MYC Brian A. Walker
  • RNA-sequencing from low-input material in multiple myeloma for application in clinical routine Anja Seckinger, Tomi Bähr-Ivacevic, Vladimir Benes, and Dirk Hose
  • Protocol for M3P : a comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma Santiago Barrio, Matteo DáVia, Laura Bruins, Thorsten Stühmer, Torsten Steinbrunn, Max Bittrich, Hermann Einsele, Alexander Keith Stewart, Esteban Braggio, and Klaus Martin Kortüm
  • Analysis of circulating tumor DNA Sridurga Mithraprabhu and Andrew Spencer
  • Detection of cross-sample contamination in multiple myeloma samples and sequencing data Owen W. Stephens, Tobias Meissner, and Niels Weinhold
  • Analysis of global gene expression profiles Alboukadel Kassambara and Jerome Moreaux
  • Genome wide mapping of methylated and hydroxyl-methylated cytosines using a modified HpaII tiny fragment enrichment by ligation mediated PCR tagged sequencing protocol Sanchari Battachariyya, Ruslana Tytarenko, Christoph Heuck, John Greally, and Amit Verma
  • Rapid and robust protocol for reduced representation bisulfite sequencing in multiple myeloma Samrat Roy Choudhury and Brian A. Walker
  • Microfluidic production and application of lipid nanoparticles for nucleic acid transfection Anitha Thomas, Shyam M. Garg, Rebecca A. G. De Souza, Eric Ouellet, Grace Tharmarajah, Dave Reichert, Mina Ordobadi, Shell Ip, and Euan C. Ramsay
  • Microfluidic assembly of liposomes with tunable size and coloading capabilities Jessica R. Hoffman, Ennio Tasciotti, and Roberto Molinaro.

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