EPs7630(®) from Pelargonium sidoides increases stress resistance in Caenorhabditis elegans probably via the DAF-16/FOXO pathway
EPs7630® a water alcohol extract of the roots from Pelargonium sidoides contains several secondary metabolites including highly oxygenated coumarins, various phenolics and polyphenols. Using the DPPH assay to measure antioxidant activity a free radical scavenging activity of 14.7±0.85μg/ml (IC50) wa...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2014
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| In: |
Phytomedicine
Year: 2014, Volume: 21, Issue: 4, Pages: 547-550 |
| ISSN: | 1618-095X |
| DOI: | 10.1016/j.phymed.2013.10.027 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.phymed.2013.10.027 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0944711313004364 
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| Author Notes: | Leila Rezaizadehnajafi, Michael Wink |
| Summary: | EPs7630® a water alcohol extract of the roots from Pelargonium sidoides contains several secondary metabolites including highly oxygenated coumarins, various phenolics and polyphenols. Using the DPPH assay to measure antioxidant activity a free radical scavenging activity of 14.7±0.85μg/ml (IC50) was determined. As an in vivo model Caenorhabditis elegans was applied to study the effect of EPs7630® on stress resistance. EPs7630® treatment reduces intracellular hsp-16.2::GFP expression (induced by the pro-oxidant juglone) indicating that the secondary metabolites of EPs7630® are bioavailable and exhibit antioxidant activities in vivo. Application of EPs7630® (50μg/ml) to the transgenic mutant TJ356 induced the migration of the transcription factor DAF-16 from cytosol to the nucleus, suggesting a prominent role of DAF-16/FOXO in the daf-2 pathway for stress resistance. |
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| Item Description: | Gesehen am 11.03.2014 |
| Physical Description: | Online Resource |
| ISSN: | 1618-095X |
| DOI: | 10.1016/j.phymed.2013.10.027 |