A quantitative measure for alterations in the actin cytoskeleton investigated with automated high-throughput microscopy
The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown. Automated approaches open up the per...
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| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2010
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| In: |
Cytometry
Year: 2010, Jahrgang: 77A, Heft: 1, Pages: 52-63 |
| ISSN: | 1552-4930 |
| DOI: | 10.1002/cyto.a.20818 |
| Online-Zugang: | Resolving-System, kostenfrei, Volltext: https://doi.org/10.1002/cyto.a.20818 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.20818 
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| Verfasserangaben: | Julian Weichsel, Nikolas Herold, Maik J. Lehmann, Hans-Georg Kräusslich, Ulrich S. Schwarz |
MARC
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| 245 | 0 | 2 | |a A quantitative measure for alterations in the actin cytoskeleton investigated with automated high-throughput microscopy |c Julian Weichsel, Nikolas Herold, Maik J. Lehmann, Hans-Georg Kräusslich, Ulrich S. Schwarz |
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| 520 | |a The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown. Automated approaches open up the perspective to clarify such an issue by processing many samples in a high-throughput manner. To analyze the alterations in the actin cytoskeleton within an automated setting, large-scale image acquisition and analysis were established for JC-53 cells stained for actin. As a quantitative measure in such an automated approach, we suggest a parameter called image coherency. We successfully benchmarked our analysis by calculating coherency for both a biophysical model of the actin cytoskeleton and for cells whose actin architecture had been disturbed pharmacologically by latrunculin B or cytochalasin D. We then tested the influence of HIV-1 infection on actin coherency, but observed no significant differences between uninfected and infected cells. © 2009 International Society for Advancement of Cytometry | ||
| 650 | 4 | |a actin cytoskeleton | |
| 650 | 4 | |a biophysical modeling | |
| 650 | 4 | |a high-throughput microscopy | |
| 650 | 4 | |a HIV-infection | |
| 650 | 4 | |a image analysis | |
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