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A quantitative measure for alterations in the actin cytoskeleton investigated with automated high-throughput microscopy

The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown. Automated approaches open up the per...

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Hauptverfasser: Weichsel, Julian (VerfasserIn) , Herold, Nikolas (VerfasserIn) , Lehmann, Maik J. (VerfasserIn) , Kräusslich, Hans-Georg (VerfasserIn) , Schwarz, Ulrich S. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2010
In: Cytometry
Year: 2010, Jahrgang: 77A, Heft: 1, Pages: 52-63
ISSN:1552-4930
DOI:10.1002/cyto.a.20818
Online-Zugang:Resolving-System, kostenfrei, Volltext: https://doi.org/10.1002/cyto.a.20818
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.20818
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Verfasserangaben:Julian Weichsel, Nikolas Herold, Maik J. Lehmann, Hans-Georg Kräusslich, Ulrich S. Schwarz
Beschreibung
Zusammenfassung:The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown. Automated approaches open up the perspective to clarify such an issue by processing many samples in a high-throughput manner. To analyze the alterations in the actin cytoskeleton within an automated setting, large-scale image acquisition and analysis were established for JC-53 cells stained for actin. As a quantitative measure in such an automated approach, we suggest a parameter called image coherency. We successfully benchmarked our analysis by calculating coherency for both a biophysical model of the actin cytoskeleton and for cells whose actin architecture had been disturbed pharmacologically by latrunculin B or cytochalasin D. We then tested the influence of HIV-1 infection on actin coherency, but observed no significant differences between uninfected and infected cells. © 2009 International Society for Advancement of Cytometry
Beschreibung:Online veröffentlicht am 6. November 2009
Gesehen am 18.10.2023
Beschreibung:Online Resource
ISSN:1552-4930
DOI:10.1002/cyto.a.20818

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