Identification of a potent neurotrophic substance for ciliary ganglionic neurons in fetal calf serum as insulin-like growth factor II
When fetal calf serum (FCS) alone is used as a trophic support for cultured chicken parasympathetic ciliary ganglionic (cCG) neurons, it does not show any survival-promoting effects on these neurons. When FCS is applied to heparin-affinity chromatography, however, potent survival-promoting activity...
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| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 May 1999
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| In: |
Journal of neuroscience research
Year: 1999, Jahrgang: 56, Heft: 4, Pages: 386-396 |
| ISSN: | 1097-4547 |
| DOI: | 10.1002/(SICI)1097-4547(19990515)56:4<386::AID-JNR6>3.0.CO;2-D |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1002/(SICI)1097-4547(19990515)56:4<386::AID-JNR6>3.0.CO;2-D
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| Verfasserangaben: | Toshiyuki Motoike and Klaus Unsicker |
MARC
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| 245 | 1 | 0 | |a Identification of a potent neurotrophic substance for ciliary ganglionic neurons in fetal calf serum as insulin-like growth factor II |c Toshiyuki Motoike and Klaus Unsicker |
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| 520 | |a When fetal calf serum (FCS) alone is used as a trophic support for cultured chicken parasympathetic ciliary ganglionic (cCG) neurons, it does not show any survival-promoting effects on these neurons. When FCS is applied to heparin-affinity chromatography, however, potent survival-promoting activity is obtained in the fraction eluted with 0.5 M NaCl. Using cCG neurons as a bioassay system, this neurotrophic activity was purified by a combination of heparin-affinity chromatography, gel filtration chromatography, and Sep-Pak C18 cartridge. The 40-50-kDa fractions from the gel filtration column with strong survival-promoting activity were shown to contain insulin-like growth factor II (IGF-II) by immunoblot analysis. By acidification, the survival-promoting activity and IGF-II were translocated together from the 40-50-kDa to the 7-10-kDa fractions, and the survival-promoting activity in the 7-10-kDa fractions was blocked by an anti-IGF-II neutralizing monoclonal antibody. These results indicate that the neurotrophic substance in 0.5 M NaCl-eluate from heparin-affinity chromatography is IGF-II and that mechanisms may exist in vivo for the activation of latent IGF-II, whose biological effects may be blocked by its specific binding proteins. J. Neurosci. Res. 56:386-396, 1999. © 1999 Wiley-Liss, Inc. | ||
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