High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosa
Dystrophic epidermolysis bullosa (DEB) is an incurable skin fragility disorder caused by mutations in the COL7A1 gene, coding for the anchoring fibril protein collagen VII (C7). Life-long mechanosensitivity of skin and mucosal surfaces is associated with large body surface erosions, chronic wounds,...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
August 2015
|
| In: |
Molecular therapy. Methods & clinical development
Year: 2015, Volume: 23, Issue: 8, Pages: 1368-1379 |
| ISSN: | 2329-0501 |
| DOI: | 10.1038/mt.2015.58 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/mt.2015.58 Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S1525001616301721?via%3Dihub 
|
| Author Notes: | Tobias Kühl, Markus Mezger, Ingrid Hausser, Rupert Handgretinger, Leena Bruckner-Tuderman and Alexander Nyström |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1552431819 | ||
| 003 | DE-627 | ||
| 005 | 20240930140422.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170127s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/mt.2015.58 |2 doi | |
| 035 | |a (DE-627)1552431819 | ||
| 035 | |a (DE-576)482431814 | ||
| 035 | |a (DE-599)BSZ482431814 | ||
| 035 | |a (OCoLC)1340956082 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Kühl, Tobias Hans-Jürgen |d 1987- |e VerfasserIn |0 (DE-588)1081264004 |0 (DE-627)845730819 |0 (DE-576)45407557X |4 aut | |
| 245 | 1 | 0 | |a High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosa |c Tobias Kühl, Markus Mezger, Ingrid Hausser, Rupert Handgretinger, Leena Bruckner-Tuderman and Alexander Nyström |
| 264 | 1 | |c August 2015 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.01.2017 | ||
| 520 | |a Dystrophic epidermolysis bullosa (DEB) is an incurable skin fragility disorder caused by mutations in the COL7A1 gene, coding for the anchoring fibril protein collagen VII (C7). Life-long mechanosensitivity of skin and mucosal surfaces is associated with large body surface erosions, chronic wounds, and secondary fibrosis that severely impede functionality. Here, we present the first systematic long-term evaluation of the therapeutic potential of a mesenchymal stromal cell (MSC)-based therapy for DEB. Intradermal administration of MSCs in a DEB mouse model resulted in production and deposition of C7 at the dermal-epidermal junction, the physiological site of function. The effect was dose-dependent with MSCs being up to 10-fold more potent than dermal fibroblasts. MSCs promoted regeneration of DEB wounds via normalization of dermal and epidermal healing and improved skin integrity through de novo formation of functional immature anchoring fibrils. Additional benefits were gained by MSCs' anti-inflammatory effects, which led to decreased immune cell infiltration into injured DEB skin. In our setting, the clinical benefit of MSC injections lasted for more than 3 months. We conclude that MSCs are viable options for localized DEB therapy. Importantly, however, the cell number needed to achieve therapeutic efficacy excludes the use of systemic administration. | ||
| 700 | 1 | |a Haußer-Siller, Ingrid |d 1957- |e VerfasserIn |0 (DE-588)1058096710 |0 (DE-627)796384703 |0 (DE-576)163782377 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular therapy. Methods & clinical development |d New York, NY : Nature Publishing Group, 2014 |g 23(2015), 8, Seite 1368-1379 |h Online-Ressource |w (DE-627)863823203 |w (DE-600)2863173-0 |w (DE-576)475381483 |x 2329-0501 |7 nnas |a High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosa |
| 773 | 1 | 8 | |g volume:23 |g year:2015 |g number:8 |g pages:1368-1379 |g extent:12 |a High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosa |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/mt.2015.58 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S1525001616301721?via%3Dihub |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170127 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 1058096710 |a Haußer-Siller, Ingrid |m 1058096710:Haußer-Siller, Ingrid |d 910000 |d 912000 |e 910000PH1058096710 |e 912000PH1058096710 |k 0/910000/ |k 1/910000/912000/ |p 3 | ||
| 999 | |a KXP-PPN1552431819 |e 295216830X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"12 S."}],"person":[{"given":"Tobias Hans-Jürgen","roleDisplay":"VerfasserIn","role":"aut","display":"Kühl, Tobias Hans-Jürgen","family":"Kühl"},{"role":"aut","roleDisplay":"VerfasserIn","given":"Ingrid","display":"Haußer-Siller, Ingrid","family":"Haußer-Siller"}],"title":[{"title_sort":"High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosa","title":"High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosa"}],"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"August 2015"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1552431819","language":["eng"],"name":{"displayForm":["Tobias Kühl, Markus Mezger, Ingrid Hausser, Rupert Handgretinger, Leena Bruckner-Tuderman and Alexander Nyström"]},"relHost":[{"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosaMolecular therapy. Methods & clinical development","physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 25.06.20"],"id":{"issn":["2329-0501"],"zdb":["2863173-0"],"eki":["863823203"]},"recId":"863823203","origin":[{"publisher":"Nature Publishing Group","dateIssuedDisp":"[2014]-","publisherPlace":"New York, NY"}],"title":[{"title_sort":"Molecular therapy","title":"Molecular therapy","partname":"Methods & clinical development"}],"titleAlt":[{"title":"Methods and clinical development"}],"pubHistory":["1 (08. Jan. 2014)-"],"part":{"text":"23(2015), 8, Seite 1368-1379","pages":"1368-1379","issue":"8","extent":"12","year":"2015","volume":"23"},"language":["eng"]}],"id":{"doi":["10.1038/mt.2015.58"],"eki":["1552431819"]},"note":["Gesehen am 27.01.2017"]} | ||
| SRT | |a KUEHLTOBIAHIGHLOCALC2015 | ||