Cytoskeletal interference: a new mode of action for the anticancer drugs camptothecin and topotecan
The anticancer drugs camptothecin (CPT) and topotecan (TPT) are known DNA topoisomerase I inhibitors which cause DNA damage and lead to cell death. In this study we provide evidence that CPT and TPT also interfere with the elements of cytoskeleton ― microtubules and actin filaments which could be pa...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
27 July 2016
|
| In: |
European journal of pharmacology
Year: 2016, Jahrgang: 789, Pages: 265-274 |
| ISSN: | 1879-0712 |
| DOI: | 10.1016/j.ejphar.2016.07.044 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.ejphar.2016.07.044 Verlag, Volltext: https://www.sciencedirect.com/science/article/pii/S001429991630485X 
|
| Verfasserangaben: | Xiaojuan Wang, Mine Tanaka, Sonja Krstin, Herbenya Silva Peixoto, Carina Carneiro de Melo Moura, Michael Wink |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1553073444 | ||
| 003 | DE-627 | ||
| 005 | 20230426141215.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170202s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ejphar.2016.07.044 |2 doi | |
| 035 | |a (DE-627)1553073444 | ||
| 035 | |a (DE-576)48307344X | ||
| 035 | |a (DE-599)BSZ48307344X | ||
| 035 | |a (OCoLC)1340955912 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Wang, Xiaojuan |e VerfasserIn |0 (DE-588)1082261246 |0 (DE-627)847270750 |0 (DE-576)45530288X |4 aut | |
| 245 | 1 | 0 | |a Cytoskeletal interference |b a new mode of action for the anticancer drugs camptothecin and topotecan |c Xiaojuan Wang, Mine Tanaka, Sonja Krstin, Herbenya Silva Peixoto, Carina Carneiro de Melo Moura, Michael Wink |
| 264 | 1 | |c 27 July 2016 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 02.02.2017 | ||
| 520 | |a The anticancer drugs camptothecin (CPT) and topotecan (TPT) are known DNA topoisomerase I inhibitors which cause DNA damage and lead to cell death. In this study we provide evidence that CPT and TPT also interfere with the elements of cytoskeleton ― microtubules and actin filaments which could be partly responsible for their cytotoxic properties. CPT and TPT apparently affected microtubule structures in living cells (Hela and U2OS) and inhibited tubulin polymerization in vitro with IC50 values of 74.57±9.96 µM and 121.55±58.68 µM, respectively. TPT significantly affected the nucleation and growth phase during the microtubule assembly in vitro, whereas the mode of action of CPT was different in that it specifically affected the ‘tread milling’ of polymerized microtubules. Cell cycle effects of CPT and TPT varied with their concentrations. CPT and TPT induced G2/M arrest and promoted the population to 76.94±11.20% and 83.91±2.43% at a concentration of 9.4 nM and 46.9 nM, respectively. As the concentration increased, cells were blocked in S phase with a dose-dependent reduction in G2/M population. In addition, CPT and TPT exhibited a certain effect on actin filaments by reducing the mass of actin filaments. The interactions of CPT and TPT with microtubules and actin filaments present new insights into their modes of action. | ||
| 650 | 4 | |a Actin filament | |
| 650 | 4 | |a Camptothecin | |
| 650 | 4 | |a DNA topoisomerase | |
| 650 | 4 | |a Microtubule | |
| 650 | 4 | |a Topotecan | |
| 700 | 1 | |a Tanaka, Mine |e VerfasserIn |0 (DE-588)1104944464 |0 (DE-627)862369819 |0 (DE-576)472957856 |4 aut | |
| 700 | 1 | |a Krstin, Sonja |e VerfasserIn |0 (DE-588)1099729076 |0 (DE-627)858744465 |0 (DE-576)469341386 |4 aut | |
| 700 | 1 | |a Peixoto, Herbenya |e VerfasserIn |0 (DE-588)1099728886 |0 (DE-627)858744279 |0 (DE-576)469341424 |4 aut | |
| 700 | 1 | |a Moura, Carina Carneiro de Melo |e VerfasserIn |0 (DE-588)1124628622 |0 (DE-627)878867775 |0 (DE-576)483074764 |4 aut | |
| 700 | 1 | |a Wink, Michael |d 1951- |e VerfasserIn |0 (DE-588)124618146 |0 (DE-627)363448519 |0 (DE-576)164659390 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of pharmacology |d New York, NY [u.a.] : Elsevier, 1967 |g 789(2016), Seite 265-274 |h Online-Ressource |w (DE-627)300897340 |w (DE-600)1483526-5 |w (DE-576)081952627 |x 1879-0712 |7 nnas |a Cytoskeletal interference a new mode of action for the anticancer drugs camptothecin and topotecan |
| 773 | 1 | 8 | |g volume:789 |g year:2016 |g pages:265-274 |g extent:10 |a Cytoskeletal interference a new mode of action for the anticancer drugs camptothecin and topotecan |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ejphar.2016.07.044 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S001429991630485X |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170202 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 124618146 |a Wink, Michael |m 124618146:Wink, Michael |d 160000 |d 160100 |d 700000 |d 729400 |e 160000PW124618146 |e 160100PW124618146 |e 700000PW124618146 |e 729400PW124618146 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/729400/ |p 6 |y j | ||
| 998 | |g 1124628622 |a Moura, Carina Carneiro de Melo |m 1124628622:Moura, Carina Carneiro de Melo |d 160000 |d 160100 |e 160000PM1124628622 |e 160100PM1124628622 |k 0/160000/ |k 1/160000/160100/ |p 5 | ||
| 998 | |g 1099728886 |a Peixoto, Herbenya |m 1099728886:Peixoto, Herbenya |d 160000 |d 160100 |e 160000PP1099728886 |e 160100PP1099728886 |k 0/160000/ |k 1/160000/160100/ |p 4 | ||
| 998 | |g 1099729076 |a Krstin, Sonja |m 1099729076:Krstin, Sonja |d 160000 |d 160100 |e 160000PK1099729076 |e 160100PK1099729076 |k 0/160000/ |k 1/160000/160100/ |p 3 | ||
| 998 | |g 1104944464 |a Tanaka, Mine |m 1104944464:Tanaka, Mine |d 160000 |d 160100 |e 160000PT1104944464 |e 160100PT1104944464 |k 0/160000/ |k 1/160000/160100/ |p 2 | ||
| 998 | |g 1082261246 |a Wang, Xiaojuan |m 1082261246:Wang, Xiaojuan |d 160000 |d 160100 |e 160000PW1082261246 |e 160100PW1082261246 |k 0/160000/ |k 1/160000/160100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1553073444 |e 2953160639 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1553073444","physDesc":[{"extent":"10 S."}],"origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"27 July 2016"}],"person":[{"given":"Xiaojuan","role":"aut","family":"Wang","display":"Wang, Xiaojuan"},{"family":"Tanaka","role":"aut","given":"Mine","display":"Tanaka, Mine"},{"family":"Krstin","role":"aut","given":"Sonja","display":"Krstin, Sonja"},{"display":"Peixoto, Herbenya","family":"Peixoto","role":"aut","given":"Herbenya"},{"display":"Moura, Carina Carneiro de Melo","family":"Moura","role":"aut","given":"Carina Carneiro de Melo"},{"role":"aut","given":"Michael","family":"Wink","display":"Wink, Michael"}],"relHost":[{"origin":[{"publisher":"Elsevier","dateIssuedKey":"1967","dateIssuedDisp":"1967-","publisherPlace":"New York, NY [u.a.]"}],"pubHistory":["1.1967 -"],"titleAlt":[{"title":"EJP"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"language":["eng"],"id":{"zdb":["1483526-5"],"issn":["1879-0712"],"eki":["300897340"]},"title":[{"title":"European journal of pharmacology","title_sort":"European journal of pharmacology","subtitle":"EJP"}],"part":{"pages":"265-274","year":"2016","text":"789(2016), Seite 265-274","volume":"789","extent":"10"},"physDesc":[{"extent":"Online-Ressource"}],"recId":"300897340","disp":"Cytoskeletal interference a new mode of action for the anticancer drugs camptothecin and topotecanEuropean journal of pharmacology"}],"title":[{"subtitle":"a new mode of action for the anticancer drugs camptothecin and topotecan","title_sort":"Cytoskeletal interference","title":"Cytoskeletal interference"}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 02.02.2017"],"name":{"displayForm":["Xiaojuan Wang, Mine Tanaka, Sonja Krstin, Herbenya Silva Peixoto, Carina Carneiro de Melo Moura, Michael Wink"]},"id":{"eki":["1553073444"],"doi":["10.1016/j.ejphar.2016.07.044"]}} | ||
| SRT | |a WANGXIAOJUCYTOSKELET2720 | ||