Stratification of pancreatic tissue samples for molecular studies: RNA-based cellular annotation procedure
Background/objectives Meaningful profiling of pancreatic cancer samples is particularly challenging due to their complex cellular composition. Beyond tumor cells, surgical biopsies contain desmoplastic stroma with infiltrating inflammatory cells, adjacent normal parenchyma, and “non-pancreatic tissu...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July-August 2015
|
| In: |
Pancreatology
Year: 2015, Jahrgang: 15, Heft: 4, Pages: 423-431 |
| ISSN: | 1424-3911 |
| DOI: | 10.1016/j.pan.2015.05.480 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.pan.2015.05.480 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1424390315005918 
|
| Verfasserangaben: | Anette Heller, Matthias M. Gaida, D. Männle, Thomas Giese, Aldo Scarpa, John P. Neoptolemos, Thilo Hackert, Oliver Strobel, Jörg D. Hoheisel, Nathalia A. Giese, Andrea S. Bauer |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1556012888 | ||
| 003 | DE-627 | ||
| 005 | 20220813152853.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170328s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.pan.2015.05.480 |2 doi | |
| 035 | |a (DE-627)1556012888 | ||
| 035 | |a (DE-576)486012883 | ||
| 035 | |a (DE-599)BSZ486012883 | ||
| 035 | |a (OCoLC)1340968890 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Heller, Anette |e VerfasserIn |0 (DE-588)1033180734 |0 (DE-627)740119443 |0 (DE-576)380768488 |4 aut | |
| 245 | 1 | 0 | |a Stratification of pancreatic tissue samples for molecular studies |b RNA-based cellular annotation procedure |c Anette Heller, Matthias M. Gaida, D. Männle, Thomas Giese, Aldo Scarpa, John P. Neoptolemos, Thilo Hackert, Oliver Strobel, Jörg D. Hoheisel, Nathalia A. Giese, Andrea S. Bauer |
| 264 | 1 | |c July-August 2015 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 28.03.2017 | ||
| 520 | |a Background/objectives Meaningful profiling of pancreatic cancer samples is particularly challenging due to their complex cellular composition. Beyond tumor cells, surgical biopsies contain desmoplastic stroma with infiltrating inflammatory cells, adjacent normal parenchyma, and “non-pancreatic tissues”. The risk of misinterpretation rises when the heterogeneous cancer tissues are sub-divided into smaller fragments for multiple analytic procedures. Pre-analytic histological evaluation is the best option to characterize pancreatic tissue samples. Our aim was to develop a complement or alternative procedure to determine the cellular composition of pancreatic cancerous biopsies, basing on intra-analytic molecular annotation. A standard process for sample stratification at a molecular level does not yet exist. Particularly in the case of retrospective or data depository-based studies, when hematoxylin-eosin stained sections are not available, it supports the correct interpretation of expression profiles. Methods A five-gene transcriptional signature (RNACellStrat) was defined that allows cell type-specific stratification of pancreatic tissues. Testing biopsy material from biobanks with this procedure demonstrated high correspondence of molecular (qRT-PCR and microarray) and histologic (hematoxylin-eosin stain) evaluations. Results Notably, about a quarter of randomly selected samples (tissue fragments) were exposed as inappropriate for subsequent clinico-pathological interpretation. Conclusions Via immediate intra-analytical procedure, our RNA-based stratification RNACellStrat increases the accuracy and reliability of the conclusions drawn from diagnostic and prognostic molecular information. | ||
| 650 | 4 | |a Cellular annotation | |
| 650 | 4 | |a Microarray analysis | |
| 650 | 4 | |a Pancreatic cancer | |
| 650 | 4 | |a qRT-PCR | |
| 650 | 4 | |a Survival analysis | |
| 650 | 4 | |a Tissue sample quality | |
| 700 | 1 | |a Gaida, Matthias |d 1982- |e VerfasserIn |0 (DE-588)139102620 |0 (DE-627)703207377 |0 (DE-576)309804523 |4 aut | |
| 700 | 1 | |a Männle, David |d 1988- |e VerfasserIn |0 (DE-588)1124103198 |0 (DE-627)87782603X |0 (DE-576)48230300X |4 aut | |
| 700 | 1 | |a Giese, Thomas |d 1962- |e VerfasserIn |0 (DE-588)143133543 |0 (DE-627)704399075 |0 (DE-576)333724496 |4 aut | |
| 700 | 1 | |a Hackert, Thilo |d 1971- |e VerfasserIn |0 (DE-588)122158512 |0 (DE-627)70579346X |0 (DE-576)182659208 |4 aut | |
| 700 | 1 | |a Strobel, Oliver |d 1974- |e VerfasserIn |0 (DE-588)123658977 |0 (DE-627)706385101 |0 (DE-576)293812357 |4 aut | |
| 700 | 1 | |a Hoheisel, Jörg D. |d 1958- |e VerfasserIn |0 (DE-588)1128674645 |0 (DE-627)883097397 |0 (DE-576)486011305 |4 aut | |
| 700 | 1 | |a Giese, Nathalia |e VerfasserIn |0 (DE-588)1033180955 |0 (DE-627)740120018 |0 (DE-576)380769123 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pancreatology |d Amsterdam : Elsevier, 2001 |g 15(2015), 4, Seite 423-431 |h Online-Ressource |w (DE-627)32764432X |w (DE-600)2043694-4 |w (DE-576)110513657 |x 1424-3911 |7 nnas |a Stratification of pancreatic tissue samples for molecular studies RNA-based cellular annotation procedure |
| 773 | 1 | 8 | |g volume:15 |g year:2015 |g number:4 |g pages:423-431 |g extent:9 |a Stratification of pancreatic tissue samples for molecular studies RNA-based cellular annotation procedure |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.pan.2015.05.480 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1424390315005918 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170328 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 1033180955 |a Giese, Nathalia |m 1033180955:Giese, Nathalia |d 910000 |d 910200 |e 910000PG1033180955 |e 910200PG1033180955 |k 0/910000/ |k 1/910000/910200/ |p 10 | ||
| 998 | |g 1128674645 |a Hoheisel, Jörg D. |m 1128674645:Hoheisel, Jörg D. |d 140000 |e 140000PH1128674645 |k 0/140000/ |p 9 | ||
| 998 | |g 123658977 |a Strobel, Oliver |m 123658977:Strobel, Oliver |d 910000 |d 910200 |e 910000PS123658977 |e 910200PS123658977 |k 0/910000/ |k 1/910000/910200/ |p 8 | ||
| 998 | |g 122158512 |a Hackert, Thilo |m 122158512:Hackert, Thilo |d 910000 |d 910200 |e 910000PH122158512 |e 910200PH122158512 |k 0/910000/ |k 1/910000/910200/ |p 7 | ||
| 998 | |g 143133543 |a Giese, Thomas |m 143133543:Giese, Thomas |d 910000 |d 911600 |e 910000PG143133543 |e 911600PG143133543 |k 0/910000/ |k 1/910000/911600/ |p 4 | ||
| 998 | |g 1124103198 |a Männle, David |m 1124103198:Männle, David |d 50000 |e 50000PM1124103198 |k 0/50000/ |p 3 | ||
| 998 | |g 139102620 |a Gaida, Matthias |m 139102620:Gaida, Matthias |d 910000 |d 912000 |e 910000PG139102620 |e 912000PG139102620 |k 0/910000/ |k 1/910000/912000/ |p 2 | ||
| 998 | |g 1033180734 |a Heller, Anette |m 1033180734:Heller, Anette |d 910000 |d 910200 |e 910000PH1033180734 |e 910200PH1033180734 |k 0/910000/ |k 1/910000/910200/ |p 1 |x j | ||
| 999 | |a KXP-PPN1556012888 |e 2962782353 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Anette Heller, Matthias M. Gaida, D. Männle, Thomas Giese, Aldo Scarpa, John P. Neoptolemos, Thilo Hackert, Oliver Strobel, Jörg D. Hoheisel, Nathalia A. Giese, Andrea S. Bauer"]},"id":{"doi":["10.1016/j.pan.2015.05.480"],"eki":["1556012888"]},"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"July-August 2015"}],"person":[{"role":"aut","given":"Anette","family":"Heller","display":"Heller, Anette"},{"role":"aut","given":"Matthias","family":"Gaida","display":"Gaida, Matthias"},{"family":"Männle","role":"aut","given":"David","display":"Männle, David"},{"given":"Thomas","role":"aut","family":"Giese","display":"Giese, Thomas"},{"family":"Hackert","role":"aut","given":"Thilo","display":"Hackert, Thilo"},{"display":"Strobel, Oliver","role":"aut","given":"Oliver","family":"Strobel"},{"display":"Hoheisel, Jörg D.","given":"Jörg D.","role":"aut","family":"Hoheisel"},{"role":"aut","given":"Nathalia","family":"Giese","display":"Giese, Nathalia"}],"relHost":[{"disp":"Stratification of pancreatic tissue samples for molecular studies RNA-based cellular annotation procedurePancreatology","recId":"32764432X","physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"official journal of the International Association of Pancreatology (IAP) and the European Pancreatic Club (EPC)","title":"Pancreatology","title_sort":"Pancreatology"}],"part":{"year":"2015","volume":"15","pages":"423-431","issue":"4","extent":"9","text":"15(2015), 4, Seite 423-431"},"id":{"issn":["1424-3911"],"eki":["32764432X"],"zdb":["2043694-4"]},"note":["Gesehen am 09.01.2019"],"type":{"bibl":"periodical","media":"Online-Ressource"},"language":["eng"],"pubHistory":["1.2001 -"],"origin":[{"dateIssuedKey":"2001","dateIssuedDisp":"2001-","publisherPlace":"Amsterdam ; Basel","publisher":"Elsevier ; Karger"}]}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 28.03.2017"],"title":[{"title_sort":"Stratification of pancreatic tissue samples for molecular studies","title":"Stratification of pancreatic tissue samples for molecular studies","subtitle":"RNA-based cellular annotation procedure"}],"physDesc":[{"extent":"9 S."}],"recId":"1556012888"} | ||
| SRT | |a HELLERANETSTRATIFICA2015 | ||