Presence of a trs-like motif promotes rep-mediated wild-type adeno-associated virus type 2 integration
High-throughput integration site (IS) analysis of wild-type adeno-associated virus type 2 (wtAAV2) in human dermal fibroblasts (HDFs) and HeLa cells revealed that juxtaposition of a Rep binding site (RBS) and terminal resolution site (trs)-like motif leads to a 4-fold-increased probability of wtAAV...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 2015
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| In: |
Journal of virology
Year: 2015, Jahrgang: 89, Heft: 14, Pages: 7428-7432 |
| ISSN: | 1098-5514 |
| DOI: | 10.1128/JVI.00426-15 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1128/JVI.00426-15 Verlag, kostenfrei, Volltext: https://journals.asm.org/doi/10.1128/jvi.00426-15 
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| Verfasserangaben: | Karl Petri, Richard Gabriel, Leticia Agundez, Raffaele Fronza, Saira Afzal, Christine Kaeppel, R. Michael Linden, Els Henckaerts, Manfred Schmidt |
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| 520 | |a High-throughput integration site (IS) analysis of wild-type adeno-associated virus type 2 (wtAAV2) in human dermal fibroblasts (HDFs) and HeLa cells revealed that juxtaposition of a Rep binding site (RBS) and terminal resolution site (trs)-like motif leads to a 4-fold-increased probability of wtAAV integration. Electrophoretic mobility shift assays (EMSAs) confirmed binding of Rep to off-target RBSs. For the first time, we show Rep protein off-target nicking activity, highlighting the importance of the nicking substrate for Rep-mediated integration. | ||
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