Evidence for different ABC-transporters in caco-2 cells modulating drug uptake
Purpose. Secretory systems contribute to drug absorption in the gastrointestinal tract. The purpose of this study was the identification of members of the ATP binding cassette superfamily of secretory transport proteins that may potentially modulate drug absorption in Caco-2 cells, which are an impo...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1999
|
| In: |
Pharmaceutical research
Year: 1999, Jahrgang: 16, Heft: 3, Pages: 402-407 |
| ISSN: | 1573-904X |
| DOI: | 10.1023/A:1018825819249 |
| Online-Zugang: | Verlag, kostenfrei registrierungspflichtig, Volltext: http://dx.doi.org/10.1023/A:1018825819249 Verlag, kostenfrei registrierungspflichtig, Volltext: https://link.springer.com/article/10.1023/A:1018825819249 
|
| Verfasserangaben: | Heike Gutmann, Gert Fricker, Michael Török, Susanne Michael, Christoph Beglinger, and Jürgen Drewe |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1556981538 | ||
| 003 | DE-627 | ||
| 005 | 20230426153700.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170426s1999 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1023/A:1018825819249 |2 doi | |
| 035 | |a (DE-627)1556981538 | ||
| 035 | |a (DE-576)486981533 | ||
| 035 | |a (DE-599)BSZ486981533 | ||
| 035 | |a (OCoLC)1340973288 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Gutmann, Heike |e VerfasserIn |0 (DE-588)113072946X |0 (DE-627)885093720 |0 (DE-576)486979202 |4 aut | |
| 245 | 1 | 0 | |a Evidence for different ABC-transporters in caco-2 cells modulating drug uptake |c Heike Gutmann, Gert Fricker, Michael Török, Susanne Michael, Christoph Beglinger, and Jürgen Drewe |
| 264 | 1 | |c 1999 | |
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 26.04.2017 | ||
| 520 | |a Purpose. Secretory systems contribute to drug absorption in the gastrointestinal tract. The purpose of this study was the identification of members of the ATP binding cassette superfamily of secretory transport proteins that may potentially modulate drug absorption in Caco-2 cells, which are an important cellular model predicting enteral absorption of drugs.Methods. Kinetic studies as well as PCR- and Western blot studies with confluent epithelial layers of human Caco-2 cells.Results. The study demonstrates functional expression of multidrug resistance related protein (MRP) and P-glycoprotein (P-gp) in Caco-2 cells: 1) Efflux studies with the MRP specific substrate glutathion-methylfluorescein (GS-MF) showed functional activity of MRP in Caco-2 cells preloaded with the metabolic precursor of GS-MF, chloro-methylfluoresceine-diacetate, CMFDA. Excretion of GS-MF was decreased in presence of the MRP-blocker MK-571.2) Transport experiments with cyclosporin A demonstrated the functional activity of P-gp. Cellular accumulation was increased in presence of the P-gp blocking agent SDZ-PSC 833.3) The expression of the 190 kDa protein MRP and the 170 kDa protein P-gp in Caco-2 cells was shown by Western blot analysis with specific monoclonal antibodies. 4) The expression of MRP-mRNA in Caco-2 cells was detected by RT-PCR and compared with the MRP over-expressing cell line H69AR. MRP primers recognize specifically human MRP1 (GenBank accession number L05628), but not all other published sequences of MRP (MRP2-MRP6). P-gp expression on mRNA-level was also confirmed by RT-PCR.Conclusions. The data demonstrate that besides P-gp, multidrug resistance related protein (MRP) is functionally expressed in Caco-2 cells and contributes to the active excretion of substrates in this cell line. | ||
| 700 | 1 | |a Fricker, Gert |d 1956- |e VerfasserIn |0 (DE-588)1042227675 |0 (DE-627)768469465 |0 (DE-576)393783464 |4 aut | |
| 700 | 1 | |a Török, Michael |e VerfasserIn |0 (DE-588)1130596443 |0 (DE-627)884810216 |0 (DE-576)486854345 |4 aut | |
| 700 | 1 | |a Michael, Susanne |e VerfasserIn |0 (DE-588)113072851X |0 (DE-627)885091604 |0 (DE-576)486977749 |4 aut | |
| 700 | 1 | |a Beglinger, Christoph |d 1950- |e VerfasserIn |0 (DE-588)113146647 |0 (DE-627)517879751 |0 (DE-576)28975349X |4 aut | |
| 700 | 1 | |a Drewe, Jürgen |e VerfasserIn |0 (DE-588)1130727483 |0 (DE-627)885089022 |0 (DE-576)486975401 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pharmaceutical research |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1984 |g 16(1999), 3, Seite 402-407 |h Online-Ressource |w (DE-627)325485291 |w (DE-600)2036232-8 |w (DE-576)104082380 |x 1573-904X |7 nnas |a Evidence for different ABC-transporters in caco-2 cells modulating drug uptake |
| 773 | 1 | 8 | |g volume:16 |g year:1999 |g number:3 |g pages:402-407 |g extent:6 |a Evidence for different ABC-transporters in caco-2 cells modulating drug uptake |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1023/A:1018825819249 |x Verlag |z kostenfrei registrierungspflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://link.springer.com/article/10.1023/A:1018825819249 |x Verlag |z kostenfrei registrierungspflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170426 | ||
| 993 | |a Article | ||
| 994 | |a 1999 | ||
| 998 | |g 113072851X |a Michael, Susanne |m 113072851X:Michael, Susanne |d 170000 |d 170200 |d 160000 |d 160100 |e 170000PM113072851X |e 170200PM113072851X |e 160000PM113072851X |e 160100PM113072851X |k 0/170000/ |k 1/170000/170200/ |k 0/160000/ |k 1/160000/160100/ |p 4 | ||
| 998 | |g 1042227675 |a Fricker, Gert |m 1042227675:Fricker, Gert |d 170000 |d 170200 |d 160000 |d 160100 |e 170000PF1042227675 |e 170200PF1042227675 |e 160000PF1042227675 |e 160100PF1042227675 |k 0/170000/ |k 1/170000/170200/ |k 0/160000/ |k 1/160000/160100/ |p 2 | ||
| 999 | |a KXP-PPN1556981538 |e 2966862807 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"title":[{"title_sort":"Evidence for different ABC-transporters in caco-2 cells modulating drug uptake","title":"Evidence for different ABC-transporters in caco-2 cells modulating drug uptake"}],"recId":"1556981538","origin":[{"dateIssuedKey":"1999","dateIssuedDisp":"1999"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"relHost":[{"pubHistory":["1.1984 -"],"part":{"pages":"402-407","extent":"6","issue":"3","volume":"16","text":"16(1999), 3, Seite 402-407","year":"1999"},"language":["eng"],"title":[{"title":"Pharmaceutical research","title_sort":"Pharmaceutical research"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"dateIssuedDisp":"1984-","publisher":"Springer Science + Business Media B.V ; Kluwer","dateIssuedKey":"1984","publisherPlace":"Dordrecht [u.a.] ; Dordrecht [u.a.]"}],"recId":"325485291","note":["Gesehen am 11.03.05"],"physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["325485291"],"zdb":["2036232-8"],"issn":["1573-904X"]},"disp":"Evidence for different ABC-transporters in caco-2 cells modulating drug uptakePharmaceutical research"}],"note":["Gesehen am 26.04.2017"],"physDesc":[{"extent":"6 S."}],"id":{"doi":["10.1023/A:1018825819249"],"eki":["1556981538"]},"name":{"displayForm":["Heike Gutmann, Gert Fricker, Michael Török, Susanne Michael, Christoph Beglinger, and Jürgen Drewe"]},"person":[{"role":"aut","given":"Heike","display":"Gutmann, Heike","family":"Gutmann"},{"role":"aut","given":"Gert","display":"Fricker, Gert","family":"Fricker"},{"role":"aut","given":"Michael","display":"Török, Michael","family":"Török"},{"role":"aut","given":"Susanne","display":"Michael, Susanne","family":"Michael"},{"family":"Beglinger","display":"Beglinger, Christoph","given":"Christoph","role":"aut"},{"family":"Drewe","display":"Drewe, Jürgen","role":"aut","given":"Jürgen"}]} | ||
| SRT | |a GUTMANNHEIEVIDENCEFO1999 | ||