Continuous Molecular Evolution of Protein-Domain Structures by Single Amino Acid Changes
Summary Protein structures cluster into families of folds that can result from extremely different amino acid sequences [1]. Because the enormous amount of genetic information generates a limited number of protein folds [2], a particular domain structure often assumes numerous functions. How new pro...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
January 23, 2007
|
| In: |
Current biology
Year: 2007, Jahrgang: 17, Heft: 2, Pages: 173-178 |
| ISSN: | 1879-0445 |
| DOI: | 10.1016/j.cub.2006.10.063 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.cub.2006.10.063 Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S0960982206024444 
|
| Verfasserangaben: | Sebastian Meier, Pernille R. Jensen, Charles N. David, Jarrod Chapman, Thomas W. Holstein, Stephan Grzesiek, Suat Özbek |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558292659 | ||
| 003 | DE-627 | ||
| 005 | 20220813164807.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170508s2007 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.cub.2006.10.063 |2 doi | |
| 035 | |a (DE-627)1558292659 | ||
| 035 | |a (DE-576)488292654 | ||
| 035 | |a (DE-599)BSZ488292654 | ||
| 035 | |a (OCoLC)1340973424 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Meier, Sebastian |e VerfasserIn |0 (DE-627)1236647777 |0 (DE-576)166647772 |4 aut | |
| 245 | 1 | 0 | |a Continuous Molecular Evolution of Protein-Domain Structures by Single Amino Acid Changes |c Sebastian Meier, Pernille R. Jensen, Charles N. David, Jarrod Chapman, Thomas W. Holstein, Stephan Grzesiek, Suat Özbek |
| 264 | 1 | |c January 23, 2007 | |
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.05.2017 | ||
| 520 | |a Summary Protein structures cluster into families of folds that can result from extremely different amino acid sequences [1]. Because the enormous amount of genetic information generates a limited number of protein folds [2], a particular domain structure often assumes numerous functions. How new protein structures and new functions evolve under these limitations remains elusive. Molecular evolution may be driven by the ability of biomacromolecules to adopt multiple conformations as a bridge between different folds [3-6]. This could allow proteins to explore new structures and new tasks while part of the structural ensemble retains the initial conformation and function as a safeguard [7]. Here we show that a global structural switch can arise from single amino acid changes in cysteine-rich domains (CRD) of cnidarian nematocyst proteins. The ability of these CRDs to form two structures with different disulfide patterns from an identical cysteine pattern is distinctive [8]. By applying a structure-based mutagenesis approach, we demonstrate that a cysteine-rich domain can interconvert between two natively occurring domain structures via a bridge state containing both structures. Comparing cnidarian CRD sequences leads us to believe that the mutations we introduced to stabilize each structure reflect the birth of new protein folds in evolution. | ||
| 650 | 4 | |a EVO_ECOL | |
| 650 | 4 | |a PROTEINS | |
| 700 | 1 | |a Holstein, Thomas W. |d 1953- |e VerfasserIn |0 (DE-588)1057984620 |0 (DE-627)796251053 |0 (DE-576)413989178 |4 aut | |
| 700 | 1 | |a Özbek, Suat |d 1968- |e VerfasserIn |0 (DE-588)122090470 |0 (DE-627)70576852X |0 (DE-576)293087024 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Current biology |d Cambridge, MA : Cell Press, 1991 |g 17(2007), 2, Seite 173-178 |h Online-Ressource |w (DE-627)320593223 |w (DE-600)2019214-9 |w (DE-576)093983190 |x 1879-0445 |7 nnas |a Continuous Molecular Evolution of Protein-Domain Structures by Single Amino Acid Changes |
| 773 | 1 | 8 | |g volume:17 |g year:2007 |g number:2 |g pages:173-178 |g extent:6 |a Continuous Molecular Evolution of Protein-Domain Structures by Single Amino Acid Changes |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.cub.2006.10.063 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0960982206024444 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170508 | ||
| 993 | |a Article | ||
| 994 | |a 2007 | ||
| 998 | |g 122090470 |a Özbek, Suat |m 122090470:Özbek, Suat |d 700000 |d 721000 |e 700000PO122090470 |e 721000PO122090470 |k 0/700000/ |k 1/700000/721000/ |p 7 |y j | ||
| 998 | |g 1057984620 |a Holstein, Thomas W. |m 1057984620:Holstein, Thomas W. |d 700000 |d 721000 |e 700000PH1057984620 |e 721000PH1057984620 |k 0/700000/ |k 1/700000/721000/ |p 5 | ||
| 999 | |a KXP-PPN1558292659 |e 2968915150 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"note":["Gesehen am 08.05.2017"],"title":[{"title_sort":"Continuous Molecular Evolution of Protein-Domain Structures by Single Amino Acid Changes","title":"Continuous Molecular Evolution of Protein-Domain Structures by Single Amino Acid Changes"}],"origin":[{"dateIssuedKey":"2007","dateIssuedDisp":"January 23, 2007"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"person":[{"display":"Meier, Sebastian","family":"Meier","given":"Sebastian","role":"aut"},{"family":"Holstein","display":"Holstein, Thomas W.","given":"Thomas W.","role":"aut"},{"family":"Özbek","display":"Özbek, Suat","role":"aut","given":"Suat"}],"relHost":[{"titleAlt":[{"title":"CB"}],"note":["Gesehen am 02.08.24"],"origin":[{"publisher":"Cell Press ; Current Biology Ltd.","dateIssuedKey":"1991","dateIssuedDisp":"1991-","publisherPlace":"Cambridge, MA ; London"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"subtitle":"CB","title_sort":"Current biology","title":"Current biology"}],"part":{"volume":"17","extent":"6","pages":"173-178","text":"17(2007), 2, Seite 173-178","year":"2007","issue":"2"},"language":["eng"],"disp":"Continuous Molecular Evolution of Protein-Domain Structures by Single Amino Acid ChangesCurrent biology","id":{"eki":["320593223"],"issn":["1879-0445"],"zdb":["2019214-9"]},"physDesc":[{"extent":"Online-Ressource"}],"pubHistory":["1.1991 -"],"recId":"320593223"}],"id":{"eki":["1558292659"],"doi":["10.1016/j.cub.2006.10.063"]},"recId":"1558292659","name":{"displayForm":["Sebastian Meier, Pernille R. Jensen, Charles N. David, Jarrod Chapman, Thomas W. Holstein, Stephan Grzesiek, Suat Özbek"]},"physDesc":[{"extent":"6 S."}]} | ||
| SRT | |a MEIERSEBASCONTINUOUS2320 | ||