Germ line transformation and in vivo labeling of nuclei in Diptera: report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae)
To understand how and when developmental traits of the fruit fly Drosophila melanogaster originated during the course of insect evolution, similar traits are functionally studied in variably related satellite species. The experimental toolkit available for relevant fly models typically comprises gen...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
5 June 2015
|
| In: |
Development genes and evolution
Year: 2015, Volume: 225, Issue: 3, Pages: 179-186 |
| ISSN: | 1432-041X |
| DOI: | 10.1007/s00427-015-0504-5 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1007/s00427-015-0504-5 Verlag, kostenfrei, Volltext: https://link.springer.com/article/10.1007/s00427-015-0504-5 |
| Author Notes: | Francesca Caroti, Silvia Urbansky, Maike Wosch, Steffen Lemke |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558370897 | ||
| 003 | DE-627 | ||
| 005 | 20220813165611.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170509s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00427-015-0504-5 |2 doi | |
| 035 | |a (DE-627)1558370897 | ||
| 035 | |a (DE-576)488370892 | ||
| 035 | |a (DE-599)BSZ488370892 | ||
| 035 | |a (OCoLC)1340973813 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Caroti, Francesca |e VerfasserIn |0 (DE-588)1131662598 |0 (DE-627)88630671X |0 (DE-576)488367727 |4 aut | |
| 245 | 1 | 0 | |a Germ line transformation and in vivo labeling of nuclei in Diptera |b report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae) |c Francesca Caroti, Silvia Urbansky, Maike Wosch, Steffen Lemke |
| 264 | 1 | |c 5 June 2015 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 09.05.2017 | ||
| 520 | |a To understand how and when developmental traits of the fruit fly Drosophila melanogaster originated during the course of insect evolution, similar traits are functionally studied in variably related satellite species. The experimental toolkit available for relevant fly models typically comprises gene expression and loss as well as gain-of-function analyses. Here, we extend the set of available molecular tools to piggyBac-based germ line transformation in two satellite fly models, Megaselia abdita and Chironomus riparius. As proof-of-concept application, we used a Gateway variant of the piggyBac transposon vector pBac{3xP3-eGFPafm} to generate a transgenic line that expresses His2Av-mCherry as fluorescent nuclear reporter ubiquitously in the gastrulating embryo of M. abdita. Our results open two phylogenetically important nodes of the insect order Diptera for advanced developmental evolutionary genetics. | ||
| 700 | 1 | |a Urbansky, Silvia |e VerfasserIn |0 (DE-588)1131662989 |0 (DE-627)886307058 |0 (DE-576)488368065 |4 aut | |
| 700 | 1 | |a Fath, Maike |e VerfasserIn |0 (DE-588)1131664647 |0 (DE-627)886308801 |0 (DE-576)488369843 |4 aut | |
| 700 | 1 | |a Lemke, Steffen |d 1977- |e VerfasserIn |0 (DE-588)134032810 |0 (DE-627)559876475 |0 (DE-576)300267282 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Development genes and evolution |d Berlin : Springer, 1996 |g 225(2015), 3, Seite 179-186 |h Online-Ressource |w (DE-627)253722187 |w (DE-600)1458990-4 |w (DE-576)072372346 |x 1432-041X |7 nnas |a Germ line transformation and in vivo labeling of nuclei in Diptera report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae) |
| 773 | 1 | 8 | |g volume:225 |g year:2015 |g number:3 |g pages:179-186 |g extent:8 |a Germ line transformation and in vivo labeling of nuclei in Diptera report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae) |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00427-015-0504-5 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://link.springer.com/article/10.1007/s00427-015-0504-5 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170509 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 134032810 |a Lemke, Steffen |m 134032810:Lemke, Steffen |d 700000 |d 721000 |e 700000PL134032810 |e 721000PL134032810 |k 0/700000/ |k 1/700000/721000/ |p 4 |y j | ||
| 998 | |g 1131664647 |a Fath, Maike |m 1131664647:Fath, Maike |d 700000 |d 721000 |e 700000PF1131664647 |e 721000PF1131664647 |k 0/700000/ |k 1/700000/721000/ |p 3 | ||
| 998 | |g 1131662989 |a Urbansky, Silvia |m 1131662989:Urbansky, Silvia |d 700000 |d 721000 |e 700000PU1131662989 |e 721000PU1131662989 |k 0/700000/ |k 1/700000/721000/ |p 2 | ||
| 998 | |g 1131662598 |a Caroti, Francesca |m 1131662598:Caroti, Francesca |d 700000 |d 721000 |e 700000PC1131662598 |e 721000PC1131662598 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1558370897 |e 2969028093 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Francesca Caroti, Silvia Urbansky, Maike Wosch, Steffen Lemke"]},"id":{"doi":["10.1007/s00427-015-0504-5"],"eki":["1558370897"]},"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"5 June 2015"}],"relHost":[{"pubHistory":["Volume 206, issue 1 (June 1996)-volume 234, issue 2 (December 2024)"],"origin":[{"dateIssuedDisp":"[1996-2024]","publisherPlace":"Berlin ; Heidelberg ; Berlin ; Heidelberg ; New York, NY","publisher":"Springer ; Springer"}],"title":[{"title":"Development genes and evolution","title_sort":"Development genes and evolution"}],"part":{"issue":"3","text":"225(2015), 3, Seite 179-186","extent":"8","pages":"179-186","volume":"225","year":"2015"},"id":{"eki":["253722187"],"issn":["1432-041X"],"zdb":["1458990-4"]},"note":["Erscheint unregelmäßig","Gesehen am 24.04.2025"],"language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"253722187","physDesc":[{"extent":"Online-Ressource"}],"disp":"Germ line transformation and in vivo labeling of nuclei in Diptera report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae)Development genes and evolution"}],"person":[{"family":"Caroti","role":"aut","given":"Francesca","display":"Caroti, Francesca"},{"display":"Urbansky, Silvia","family":"Urbansky","role":"aut","given":"Silvia"},{"display":"Fath, Maike","family":"Fath","given":"Maike","role":"aut"},{"display":"Lemke, Steffen","given":"Steffen","role":"aut","family":"Lemke"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"note":["Gesehen am 09.05.2017"],"title":[{"subtitle":"report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae)","title_sort":"Germ line transformation and in vivo labeling of nuclei in Diptera","title":"Germ line transformation and in vivo labeling of nuclei in Diptera"}],"physDesc":[{"extent":"8 S."}],"recId":"1558370897"} | ||
| SRT | |a CAROTIFRANGERMLINETR5201 | ||