Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. juncea
A differential display cDNA-AFLP derived technique was used to identify gene transcripts regulated by chromium (Cr) in relation to sulfur (S) nutrition in Brassica juncea. Twelve-day old plants were grown with 200 μM sulfate (+S), without sulfate (−S), with 200 μM sulfate plus 200 μM chromate (+S +...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
1 September 2012
|
| In: |
Journal of hazardous materials
Year: 2012, Volume: 239/240, Pages: 192-205 |
| ISSN: | 1873-3336 |
| DOI: | 10.1016/j.jhazmat.2012.08.060 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.jhazmat.2012.08.060 Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S0304389412008758 
|
| Author Notes: | Michela Schiavon, Giulio Galla, Markus Wirtz, Elizabeth A.H. Pilon-Smits, Valentina Telatin, Silvia Quaggiotti, Ruediger Hell, Gianni Barcaccia, Mario Malagoli |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558535217 | ||
| 003 | DE-627 | ||
| 005 | 20220813171107.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170511s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jhazmat.2012.08.060 |2 doi | |
| 035 | |a (DE-627)1558535217 | ||
| 035 | |a (DE-576)488535212 | ||
| 035 | |a (DE-599)BSZ488535212 | ||
| 035 | |a (OCoLC)1340975265 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Schiavon, Michela |e VerfasserIn |0 (DE-588)1131833473 |0 (DE-627)886732131 |0 (DE-576)488490790 |4 aut | |
| 245 | 1 | 0 | |a Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. juncea |c Michela Schiavon, Giulio Galla, Markus Wirtz, Elizabeth A.H. Pilon-Smits, Valentina Telatin, Silvia Quaggiotti, Ruediger Hell, Gianni Barcaccia, Mario Malagoli |
| 264 | 1 | |c 1 September 2012 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 11.05.2017 | ||
| 520 | |a A differential display cDNA-AFLP derived technique was used to identify gene transcripts regulated by chromium (Cr) in relation to sulfur (S) nutrition in Brassica juncea. Twelve-day old plants were grown with 200 μM sulfate (+S), without sulfate (−S), with 200 μM sulfate plus 200 μM chromate (+S + Cr), or without sulfate plus 200 μM chromate (−S + Cr). Forty-four combinations of degenerate primers were assayed, which allowed the detection of 346 Transcript-Derived Fragments (TDFs) differentially regulated by Cr and S at times 0, 10 min, 1 h, 24 h. Eight sulfate transporters were identified, whose transcript abundance was dependent on the levels of plant S-compounds. For some of these transporters, a tight coordinated regulation of gene expression was observed in response to Cr. The MapMan analysis revealed a differential pattern of gene expression between +S + Cr and −S + Cr plants for several other transcripts and highlighted an overlap among responses to metals, defence against pathogens and senescence, hence suggesting the existence of common mechanisms of gene regulation. Among the identified gene transcripts, those involved in S metabolism and proteolitic processes may represent potential targets of genetic engineering in efforts to increase Cr accumulation and tolerance in plant species employed in phytoremediation techniques. | ||
| 650 | 4 | |a Brassica juncea | |
| 650 | 4 | |a Chromium | |
| 650 | 4 | |a Phytoremediation | |
| 650 | 4 | |a Sulfur | |
| 650 | 4 | |a Transcriptome | |
| 700 | 1 | |a Wirtz, Markus |e VerfasserIn |0 (DE-588)1023118378 |0 (DE-627)717709353 |0 (DE-576)36641979X |4 aut | |
| 700 | 1 | |a Hell, Rüdiger |e VerfasserIn |0 (DE-588)1022964933 |0 (DE-627)717340341 |0 (DE-576)166399647 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of hazardous materials |d New York, NY [u.a.] : Science Direct, 1976 |g 239/240(2012), Seite 192-205 |h Online-Ressource |w (DE-627)302467904 |w (DE-600)1491302-1 |w (DE-576)259483893 |x 1873-3336 |7 nnas |a Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. juncea |
| 773 | 1 | 8 | |g volume:239/240 |g year:2012 |g pages:192-205 |g extent:14 |a Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. juncea |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jhazmat.2012.08.060 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0304389412008758 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170511 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 1022964933 |a Hell, Rüdiger |m 1022964933:Hell, Rüdiger |d 700000 |d 721000 |e 700000PH1022964933 |e 721000PH1022964933 |k 0/700000/ |k 1/700000/721000/ |p 7 | ||
| 998 | |g 1023118378 |a Wirtz, Markus |m 1023118378:Wirtz, Markus |d 700000 |d 721000 |e 700000PW1023118378 |e 721000PW1023118378 |k 0/700000/ |k 1/700000/721000/ |p 3 | ||
| 999 | |a KXP-PPN1558535217 |e 2969286009 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"pubHistory":["1.1975/76 - 192.2011; Vol. 193.2011 -"],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Fortsetzung der Druck-Ausgabe"],"title":[{"title_sort":"Journal of hazardous materials","subtitle":"environmental control, risk assessment, impact and management","title":"Journal of hazardous materials"}],"disp":"Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. junceaJournal of hazardous materials","recId":"302467904","part":{"extent":"14","text":"239/240(2012), Seite 192-205","volume":"239/240","pages":"192-205","year":"2012"},"id":{"eki":["302467904"],"zdb":["1491302-1"],"issn":["1873-3336"]},"origin":[{"dateIssuedDisp":"1976-","publisher":"Science Direct","dateIssuedKey":"1976","publisherPlace":"New York, NY [u.a.]"}]}],"note":["Gesehen am 11.05.2017"],"origin":[{"dateIssuedDisp":"1 September 2012","dateIssuedKey":"2012"}],"id":{"eki":["1558535217"],"doi":["10.1016/j.jhazmat.2012.08.060"]},"recId":"1558535217","name":{"displayForm":["Michela Schiavon, Giulio Galla, Markus Wirtz, Elizabeth A.H. Pilon-Smits, Valentina Telatin, Silvia Quaggiotti, Ruediger Hell, Gianni Barcaccia, Mario Malagoli"]},"title":[{"title_sort":"Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. juncea","title":"Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. juncea"}],"person":[{"family":"Schiavon","given":"Michela","role":"aut","display":"Schiavon, Michela"},{"display":"Wirtz, Markus","given":"Markus","role":"aut","family":"Wirtz"},{"display":"Hell, Rüdiger","given":"Rüdiger","role":"aut","family":"Hell"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"physDesc":[{"extent":"14 S."}]} | ||
| SRT | |a SCHIAVONMITRANSCRIPT1201 | ||