Conservation of helical bundle structure between the exocyst subunits
Background The exocyst is a large hetero-octomeric protein complex required for regulating the targeting and fusion of secretory vesicles to the plasma membrane in eukaryotic cells. Although the sequence identity between the eight different exocyst subunits is less than 10%, structures of domains of...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
February 13, 2009
|
| In: |
PLOS ONE
Year: 2009, Jahrgang: 4, Heft: 2 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0004443 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1371/journal.pone.0004443 Verlag, kostenfrei, Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0004443 
|
| Verfasserangaben: | Nicole J. Croteau, Melonnie L.M. Furgason, Damien Devos, Mary Munson |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558544852 | ||
| 003 | DE-627 | ||
| 005 | 20220813171221.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170512s2009 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0004443 |2 doi | |
| 035 | |a (DE-627)1558544852 | ||
| 035 | |a (DE-576)488544858 | ||
| 035 | |a (DE-599)BSZ488544858 | ||
| 035 | |a (OCoLC)1340975325 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Croteau, Nicole |e VerfasserIn |0 (DE-588)1131882334 |0 (DE-627)886796040 |0 (DE-576)488544807 |4 aut | |
| 245 | 1 | 0 | |a Conservation of helical bundle structure between the exocyst subunits |c Nicole J. Croteau, Melonnie L.M. Furgason, Damien Devos, Mary Munson |
| 264 | 1 | |c February 13, 2009 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.05.2017 | ||
| 520 | |a Background The exocyst is a large hetero-octomeric protein complex required for regulating the targeting and fusion of secretory vesicles to the plasma membrane in eukaryotic cells. Although the sequence identity between the eight different exocyst subunits is less than 10%, structures of domains of four of the subunits revealed a similar helical bundle topology. Characterization of several of these subunits has been hindered by lack of soluble protein for biochemical and structural studies. Methodology/Principal Findings Using advanced hidden Markov models combined with secondary structure predictions, we detect significant sequence similarity between each of the exocyst subunits, indicating that they all contain helical bundle structures. We corroborate these remote homology predictions by identifying and purifying a predicted domain of yeast Sec10p, a previously insoluble exocyst subunit. This domain is soluble and folded with approximately 60% α-helicity, in agreement with our predictions, and capable of interacting with several known Sec10p binding partners. Conclusions/Significance Although all eight of the exocyst subunits had been suggested to be composed of similar helical bundles, this has now been validated by our hidden Markov model structure predictions. In addition, these predictions identified protein domains within the exocyst subunits, resulting in creation and characterization of a soluble, folded domain of Sec10p. | ||
| 650 | 4 | |a Cell membranes | |
| 650 | 4 | |a Hidden Markov models | |
| 650 | 4 | |a Membrane proteins | |
| 650 | 4 | |a Protein domains | |
| 650 | 4 | |a Protein structure | |
| 650 | 4 | |a Protein structure comparison | |
| 650 | 4 | |a Protein structure prediction | |
| 650 | 4 | |a Vesicles | |
| 700 | 1 | |a Devos, Damien |e VerfasserIn |0 (DE-588)1038353319 |0 (DE-627)766122247 |0 (DE-576)392341069 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 4(2009,2) Artikel-Nummer e4443, 8 Seiten |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a Conservation of helical bundle structure between the exocyst subunits |
| 773 | 1 | 8 | |g volume:4 |g year:2009 |g number:2 |g extent:8 |a Conservation of helical bundle structure between the exocyst subunits |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.pone.0004443 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0004443 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170512 | ||
| 993 | |a Article | ||
| 994 | |a 2009 | ||
| 998 | |g 1038353319 |a Devos, Damien |m 1038353319:Devos, Damien |p 3 | ||
| 999 | |a KXP-PPN1558544852 |e 2969310376 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 12.05.2017"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1558544852","language":["eng"],"person":[{"display":"Croteau, Nicole","roleDisplay":"VerfasserIn","role":"aut","family":"Croteau","given":"Nicole"},{"display":"Devos, Damien","roleDisplay":"VerfasserIn","role":"aut","family":"Devos","given":"Damien"}],"title":[{"title":"Conservation of helical bundle structure between the exocyst subunits","title_sort":"Conservation of helical bundle structure between the exocyst subunits"}],"physDesc":[{"extent":"8 S."}],"relHost":[{"title":[{"title_sort":"PLOS ONE","title":"PLOS ONE"}],"pubHistory":["1.2006 -"],"part":{"volume":"4","text":"4(2009,2) Artikel-Nummer e4443, 8 Seiten","extent":"8","year":"2009","issue":"2"},"note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"disp":"Conservation of helical bundle structure between the exocyst subunitsPLOS ONE","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"523574592","language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"Public Library of Science","role":"isb"}],"origin":[{"publisher":"PLOS ; PLoS","dateIssuedKey":"2006","dateIssuedDisp":"2006-","publisherPlace":"San Francisco, California, US ; Lawrence, Kan."}],"id":{"issn":["1932-6203"],"zdb":["2267670-3"],"eki":["523574592"]},"name":{"displayForm":["Public Library of Science"]},"physDesc":[{"extent":"Online-Ressource"}]}],"name":{"displayForm":["Nicole J. Croteau, Melonnie L.M. Furgason, Damien Devos, Mary Munson"]},"origin":[{"dateIssuedDisp":"February 13, 2009","dateIssuedKey":"2009"}],"id":{"eki":["1558544852"],"doi":["10.1371/journal.pone.0004443"]}} | ||
| SRT | |a CROTEAUNICCONSERVATI1320 | ||