Methods for generating year-round access to amphioxus in the laboratory
Cephalochordates, commonly known as amphioxus, are key to understanding vertebrate origins. However, laboratory work suffers from limited access to adults and embryonic material. Here we report the design and experimental validation of an inland marine facility that allows establishing stable amphio...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
August 26, 2013
|
| In: |
PLOS ONE
Year: 2013, Jahrgang: 8, Heft: 8, Pages: e71599 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0071599 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1371/journal.pone.0071599 Verlag, Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0071599 
|
| Verfasserangaben: | Èlia Benito-Gutiérrez, Hermann Weber, Diana Virginia Bryant, Detlev Arendt |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558546537 | ||
| 003 | DE-627 | ||
| 005 | 20220813171228.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170512s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0071599 |2 doi | |
| 035 | |a (DE-627)1558546537 | ||
| 035 | |a (DE-576)488546532 | ||
| 035 | |a (DE-599)BSZ488546532 | ||
| 035 | |a (OCoLC)1340973846 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Benito-Gutiérrez, Èlia |e VerfasserIn |0 (DE-588)1131881443 |0 (DE-627)886794692 |0 (DE-576)488543002 |4 aut | |
| 245 | 1 | 0 | |a Methods for generating year-round access to amphioxus in the laboratory |c Èlia Benito-Gutiérrez, Hermann Weber, Diana Virginia Bryant, Detlev Arendt |
| 264 | 1 | |c August 26, 2013 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.05.2017 | ||
| 520 | |a Cephalochordates, commonly known as amphioxus, are key to understanding vertebrate origins. However, laboratory work suffers from limited access to adults and embryonic material. Here we report the design and experimental validation of an inland marine facility that allows establishing stable amphioxus colonies in the laboratory and obtaining embryos at any time of day and over almost the entire year, far exceeding natural conditions. This is achieved by mimicking the natural benthic environment, natural day- and moon- light, natural substrate and by providing a strictly controlled and seasonally fluctuating temperature regimen. Moreover, supplemented algae diets allow animals to refill their gonads in consecutive years. Spontaneous spawning, a major problem in previous setups, no longer occurs in our facility; instead, all breeding is induced and fertilization occurs fully in vitro. Our system makes amphioxus a standard laboratory animal model. | ||
| 650 | 4 | |a Algae | |
| 650 | 4 | |a Amphioxus | |
| 650 | 4 | |a Animal husbandry | |
| 650 | 4 | |a Daylight | |
| 650 | 4 | |a Gonads | |
| 650 | 4 | |a Oxygen | |
| 650 | 4 | |a Sea water | |
| 650 | 4 | |a Spawning | |
| 700 | 1 | |a Bryant, Diana |e VerfasserIn |0 (DE-588)1131882164 |0 (DE-627)886795796 |0 (DE-576)488544378 |4 aut | |
| 700 | 1 | |a Arendt, Detlev |e VerfasserIn |0 (DE-588)113136189X |0 (DE-627)88602952X |0 (DE-576)180450417 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 8(2013,8) Artikel-Nummer e71599, 7 Seiten |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a Methods for generating year-round access to amphioxus in the laboratory |
| 773 | 1 | 8 | |g volume:8 |g year:2013 |g number:8 |g pages:e71599 |g extent:7 |a Methods for generating year-round access to amphioxus in the laboratory |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.pone.0071599 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0071599 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170512 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 113136189X |a Arendt, Detlev |m 113136189X:Arendt, Detlev |d 700000 |d 721000 |e 700000PA113136189X |e 721000PA113136189X |k 0/700000/ |k 1/700000/721000/ |p 4 |y j | ||
| 998 | |g 1131882164 |a Bryant, Diana |m 1131882164:Bryant, Diana |p 3 | ||
| 999 | |a KXP-PPN1558546537 |e 296931181X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 12.05.2017"],"recId":"1558546537","language":["eng"],"title":[{"title_sort":"Methods for generating year-round access to amphioxus in the laboratory","title":"Methods for generating year-round access to amphioxus in the laboratory"}],"person":[{"family":"Benito-Gutiérrez","given":"Èlia","roleDisplay":"VerfasserIn","display":"Benito-Gutiérrez, Èlia","role":"aut"},{"given":"Diana","family":"Bryant","role":"aut","display":"Bryant, Diana","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","display":"Arendt, Detlev","role":"aut","family":"Arendt","given":"Detlev"}],"physDesc":[{"extent":"7 S."}],"relHost":[{"id":{"zdb":["2267670-3"],"eki":["523574592"],"issn":["1932-6203"]},"origin":[{"publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","dateIssuedKey":"2006","publisher":"PLOS ; PLoS","dateIssuedDisp":"2006-"}],"name":{"displayForm":["Public Library of Science"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"PLOS ONE","title":"PLOS ONE"}],"part":{"pages":"e71599","issue":"8","year":"2013","extent":"7","text":"8(2013,8) Artikel-Nummer e71599, 7 Seiten","volume":"8"},"pubHistory":["1.2006 -"],"recId":"523574592","language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"Public Library of Science","role":"isb"}],"disp":"Methods for generating year-round access to amphioxus in the laboratoryPLOS ONE","note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"type":{"media":"Online-Ressource","bibl":"periodical"}}],"origin":[{"dateIssuedKey":"2013","dateIssuedDisp":"August 26, 2013"}],"id":{"eki":["1558546537"],"doi":["10.1371/journal.pone.0071599"]},"name":{"displayForm":["Èlia Benito-Gutiérrez, Hermann Weber, Diana Virginia Bryant, Detlev Arendt"]}} | ||
| SRT | |a BENITOGUTIMETHODSFOR2620 | ||