The three-dimensional molecular structure of the desmosomal plaque
The cytoplasmic surface of intercellular junctions is a complex network of molecular interactions that link the extracellular region of the desmosomal cadherins with the cytoskeletal intermediate filaments. Although 3D structures of the major plaque components are known, the overall architecture rem...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
April 19, 2011
|
| In: |
Proceedings of the National Academy of Sciences of the United States of America
Year: 2011, Volume: 108, Issue: 16, Pages: 6480-6485 |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.1019469108 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1073/pnas.1019469108 Verlag, kostenfrei, Volltext: http://www.pnas.org/content/108/16/6480 
|
| Author Notes: | Ashraf Al-Amoudi, Daniel Castaño-Diez, Damien P. Devos, Robert B. Russell, Graham T. Johnson, and Achilleas S. Frangakis |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558670513 | ||
| 003 | DE-627 | ||
| 005 | 20220813173023.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170516s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1073/pnas.1019469108 |2 doi | |
| 035 | |a (DE-627)1558670513 | ||
| 035 | |a (DE-576)488670519 | ||
| 035 | |a (DE-599)BSZ488670519 | ||
| 035 | |a (OCoLC)1340975479 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Al-Amoudi, Ashraf |e VerfasserIn |0 (DE-588)1132162467 |0 (DE-627)887455530 |0 (DE-576)488670276 |4 aut | |
| 245 | 1 | 4 | |a The three-dimensional molecular structure of the desmosomal plaque |c Ashraf Al-Amoudi, Daniel Castaño-Diez, Damien P. Devos, Robert B. Russell, Graham T. Johnson, and Achilleas S. Frangakis |
| 264 | 1 | |c April 19, 2011 | |
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 16.05.2017 | ||
| 520 | |a The cytoplasmic surface of intercellular junctions is a complex network of molecular interactions that link the extracellular region of the desmosomal cadherins with the cytoskeletal intermediate filaments. Although 3D structures of the major plaque components are known, the overall architecture remains unknown. We used cryoelectron tomography of vitreous sections from human epidermis to record 3D images of desmosomes in vivo and in situ at molecular resolution. Our results show that the architecture of the cytoplasmic surface of the desmosome is a 2D interconnected quasiperiodic lattice, with a similar spatial organization to the extracellular side. Subtomogram averaging of the plaque region reveals two distinct layers of the desmosomal plaque: a low-density layer closer to the membrane and a high-density layer further away from the membrane. When combined with a heuristic, allowing simultaneous constrained fitting of the high-resolution structures of the major plaque proteins (desmoplakin, plakophilin, and plakoglobin), it reveals their mutual molecular interactions and explains their stoichiometry. The arrangement suggests that alternate plakoglobin-desmoplakin complexes create a template on which desmosomal cadherins cluster before they stabilize extracellularly by binding at their N-terminal tips. Plakophilins are added as a molecular reinforcement to fill the gap between the formed plaque complexes and the plasma membrane. | ||
| 650 | 4 | |a genetic algorithms | |
| 650 | 4 | |a image processing | |
| 650 | 4 | |a multi-protein fitting | |
| 650 | 4 | |a vitreous sectioning | |
| 700 | 1 | |a Devos, Damien |e VerfasserIn |0 (DE-588)1038353319 |0 (DE-627)766122247 |0 (DE-576)392341069 |4 aut | |
| 700 | 1 | |a Russell, Robert B. |e VerfasserIn |0 (DE-588)173751091 |0 (DE-627)698656334 |0 (DE-576)134591755 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a National Academy of Sciences (Washington, DC) |t Proceedings of the National Academy of Sciences of the United States of America |d Washington, DC : National Acad. of Sciences, 1915 |g 108(2011), 16, Seite 6480-6485 |h Online-Ressource |w (DE-627)254235379 |w (DE-600)1461794-8 |w (DE-576)073260509 |x 1091-6490 |7 nnas |
| 773 | 1 | 8 | |g volume:108 |g year:2011 |g number:16 |g pages:6480-6485 |g extent:6 |a The three-dimensional molecular structure of the desmosomal plaque |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1073/pnas.1019469108 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://www.pnas.org/content/108/16/6480 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170516 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 173751091 |a Russell, Robert B. |m 173751091:Russell, Robert B. |d 910000 |d 950000 |d 950950 |e 910000PR173751091 |e 950000PR173751091 |e 950950PR173751091 |k 0/910000/ |k 1/910000/950000/ |k 2/910000/950000/950950/ |p 4 | ||
| 998 | |g 1038353319 |a Devos, Damien |m 1038353319:Devos, Damien |d 910000 |d 950000 |d 950950 |e 910000PD1038353319 |e 950000PD1038353319 |e 950950PD1038353319 |k 0/910000/ |k 1/910000/950000/ |k 2/910000/950000/950950/ |p 3 | ||
| 999 | |a KXP-PPN1558670513 |e 2969534738 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1073/pnas.1019469108"],"eki":["1558670513"]},"note":["Gesehen am 16.05.2017"],"relHost":[{"titleAlt":[{"title":"PNAS online"},{"title":"PNAS"}],"title":[{"title":"Proceedings of the National Academy of Sciences of the United States of America","title_sort":"Proceedings of the National Academy of Sciences of the United States of America"}],"physDesc":[{"extent":"Online-Ressource"}],"pubHistory":["1.1915 -"],"part":{"volume":"108","issue":"16","year":"2011","text":"108(2011), 16, Seite 6480-6485","extent":"6","pages":"6480-6485"},"disp":"National Academy of Sciences (Washington, DC)Proceedings of the National Academy of Sciences of the United States of America","id":{"zdb":["1461794-8"],"eki":["254235379"],"issn":["1091-6490"]},"recId":"254235379","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 09.03.2022"],"origin":[{"publisherPlace":"Washington, DC","dateIssuedKey":"1915","dateIssuedDisp":"1915-","publisher":"National Acad. of Sciences"}],"language":["eng"],"corporate":[{"role":"aut","display":"National Academy of Sciences"}]}],"origin":[{"dateIssuedKey":"2011","dateIssuedDisp":"April 19, 2011"}],"name":{"displayForm":["Ashraf Al-Amoudi, Daniel Castaño-Diez, Damien P. Devos, Robert B. Russell, Graham T. Johnson, and Achilleas S. Frangakis"]},"language":["eng"],"title":[{"title":"The three-dimensional molecular structure of the desmosomal plaque","title_sort":"three-dimensional molecular structure of the desmosomal plaque"}],"recId":"1558670513","physDesc":[{"extent":"6 S."}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"person":[{"role":"aut","display":"Al-Amoudi, Ashraf","given":"Ashraf","family":"Al-Amoudi"},{"role":"aut","family":"Devos","display":"Devos, Damien","given":"Damien"},{"role":"aut","given":"Robert B.","display":"Russell, Robert B.","family":"Russell"}]} | ||
| SRT | |a ALAMOUDIASTHREEDIMEN1920 | ||