Mitochondrial serine Acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells
Cysteine (Cys) synthesis in plants is carried out by two sequential reactions catalyzed by the rate-limiting enzyme serine acetyltransferase (SAT) and excess amounts of O-acetylserine(thiol)lyase. Why these reactions occur in plastids, mitochondria, and cytosol of plants remained unclear. Expression...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
August 27, 2008
|
| In: |
Plant physiology
Year: 2008, Volume: 148, Issue: 2, Pages: 1055-1067 |
| ISSN: | 1532-2548 |
| DOI: | 10.1104/pp.108.125237 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1104/pp.108.125237 Verlag, kostenfrei, Volltext: https://academic.oup.com/plphys/article/148/2/1055/6107496 
|
| Author Notes: | Florian H. Haas, Corinna Heeg, Rafael Queiroz, Andrea Bauer, Markus Wirtz and Rüdiger Hell |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 155868347X | ||
| 003 | DE-627 | ||
| 005 | 20241015120105.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170516s2008 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1104/pp.108.125237 |2 doi | |
| 035 | |a (DE-627)155868347X | ||
| 035 | |a (DE-576)488683475 | ||
| 035 | |a (DE-599)BSZ488683475 | ||
| 035 | |a (OCoLC)1340975492 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Haas, Florian Heinrich |e VerfasserIn |0 (DE-588)1019942584 |0 (DE-627)691059365 |0 (DE-576)35785649X |4 aut | |
| 245 | 1 | 0 | |a Mitochondrial serine Acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells |c Florian H. Haas, Corinna Heeg, Rafael Queiroz, Andrea Bauer, Markus Wirtz and Rüdiger Hell |
| 264 | 1 | |c August 27, 2008 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 16.05.2017 | ||
| 520 | |a Cysteine (Cys) synthesis in plants is carried out by two sequential reactions catalyzed by the rate-limiting enzyme serine acetyltransferase (SAT) and excess amounts of O-acetylserine(thiol)lyase. Why these reactions occur in plastids, mitochondria, and cytosol of plants remained unclear. Expression of artificial microRNA (amiRNA) against Sat3 encoding mitochondrial SAT3 in transgenic Arabidopsis (Arabidopsis thaliana) plants demonstrates that mitochondria are the most important compartment for the synthesis of O-acetylserine (OAS), the precursor of Cys. Reduction of RNA levels, protein contents, SAT enzymatic activity, and phenotype strongly correlate in independent amiSAT3 lines and cause significantly retarded growth. The expression of the other four Sat genes in the Arabidopsis genome are not affected by amiRNA-SAT3 according to quantitative real-time polymerase chain reaction and microarray analyses. Application of radiolabeled serine to leaf pieces revealed severely reduced incorporation rates into Cys and even more so into glutathione. Accordingly, steady-state levels of OAS are 4-fold reduced. Decrease of sulfate reduction-related genes is accompanied by an accumulation of sulfate in amiSAT3 lines. These results unequivocally show that mitochondria provide the bulk of OAS in the plant cell and are the likely site of flux regulation. Together with recent data, the cytosol appears to be a major site of Cys synthesis, while plastids contribute reduced sulfur as sulfide. Thus, Cys synthesis in plants is significantly different from that in nonphotosynthetic eukaryotes at the cellular level. | ||
| 700 | 1 | |a Heeg, Corinna |e VerfasserIn |0 (DE-588)139010203 |0 (DE-627)703179837 |0 (DE-576)30938947X |4 aut | |
| 700 | 1 | |a Wirtz, Markus |e VerfasserIn |0 (DE-588)1023118378 |0 (DE-627)717709353 |0 (DE-576)36641979X |4 aut | |
| 700 | 1 | |a Hell, Rüdiger |e VerfasserIn |0 (DE-588)1022964933 |0 (DE-627)717340341 |0 (DE-576)166399647 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Plant physiology |d Oxford : Oxford University Press, 1926 |g 148(2008), 2, Seite 1055-1067 |h Online-Ressource |w (DE-627)314092579 |w (DE-600)2004346-6 |w (DE-576)090885635 |x 1532-2548 |7 nnas |a Mitochondrial serine Acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells |
| 773 | 1 | 8 | |g volume:148 |g year:2008 |g number:2 |g pages:1055-1067 |g extent:13 |a Mitochondrial serine Acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1104/pp.108.125237 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://academic.oup.com/plphys/article/148/2/1055/6107496 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170516 | ||
| 993 | |a Article | ||
| 994 | |a 2008 | ||
| 998 | |g 1022964933 |a Hell, Rüdiger |m 1022964933:Hell, Rüdiger |d 700000 |d 721000 |e 700000PH1022964933 |e 721000PH1022964933 |k 0/700000/ |k 1/700000/721000/ |p 6 |y j | ||
| 998 | |g 1023118378 |a Wirtz, Markus |m 1023118378:Wirtz, Markus |d 910000 |d 950000 |d 950950 |e 910000PW1023118378 |e 950000PW1023118378 |e 950950PW1023118378 |k 0/910000/ |k 1/910000/950000/ |k 2/910000/950000/950950/ |p 5 | ||
| 998 | |g 139010203 |a Heeg, Corinna |m 139010203:Heeg, Corinna |d 700000 |d 721000 |e 700000PH139010203 |e 721000PH139010203 |k 0/700000/ |k 1/700000/721000/ |p 2 | ||
| 998 | |g 1019942584 |a Haas, Florian Heinrich |m 1019942584:Haas, Florian Heinrich |d 700000 |d 721000 |e 700000PH1019942584 |e 721000PH1019942584 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN155868347X |e 2969552256 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"title":[{"title":"Plant physiology","title_sort":"Plant physiology"}],"corporate":[{"role":"isb","display":"American Society of Plant Physiologists"},{"display":"American Society of Plant Biologists","role":"isb"}],"origin":[{"dateIssuedKey":"1926","publisher":"Oxford University Press ; Soc.","publisherPlace":"Oxford ; Rockville, Md.","dateIssuedDisp":"1926-"}],"id":{"zdb":["2004346-6"],"issn":["1532-2548"],"eki":["314092579"]},"name":{"displayForm":["publ. monthly by the American Society of Plant Biologists"]},"language":["eng"],"part":{"text":"148(2008), 2, Seite 1055-1067","extent":"13","year":"2008","pages":"1055-1067","volume":"148","issue":"2"},"physDesc":[{"extent":"Online-Ressource"}],"disp":"Mitochondrial serine Acetyltransferase functions as a pacemaker of cysteine synthesis in plant cellsPlant physiology","recId":"314092579","note":["Fortsetzung der Druck-Ausgabe","Gesehen am 09.08.2022","Beteil. Körp. anfangs: American Society of Plant Physiologists"],"pubHistory":["1.1926 -"],"type":{"bibl":"periodical","media":"Online-Ressource"},"titleAlt":[{"title":"A journal devoted to all phases of plant physiology"},{"title":"Volume 80, number 1 (January 1986)-volume 122, number 4 (April 2000): An international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants"},{"title":"Volume 123, number 1 (May 2000)-volume 128, number 3 (March 2002): An international journal devoted to development, cellular and molecular biology, biochemistry, biophysics, physiology, environmental biology, molecular evolution, genomics, ecophysiology, and whole plant"},{"title":"Volume 128, number 4 (April 2002)-volume 169, number 2 (October 2015): An international journal devoted to basic research into how plants function, ranging from the molecular to the cellular to the whole plant levels, and including the interactions of plants with their biotic and abiotic environments"}]}],"note":["Gesehen am 16.05.2017"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"id":{"eki":["155868347X"],"doi":["10.1104/pp.108.125237"]},"name":{"displayForm":["Florian H. Haas, Corinna Heeg, Rafael Queiroz, Andrea Bauer, Markus Wirtz and Rüdiger Hell"]},"language":["eng"],"physDesc":[{"extent":"13 S."}],"recId":"155868347X","title":[{"title":"Mitochondrial serine Acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells","title_sort":"Mitochondrial serine Acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells"}],"person":[{"given":"Florian Heinrich","family":"Haas","display":"Haas, Florian Heinrich","role":"aut"},{"family":"Heeg","role":"aut","display":"Heeg, Corinna","given":"Corinna"},{"display":"Wirtz, Markus","role":"aut","family":"Wirtz","given":"Markus"},{"given":"Rüdiger","role":"aut","display":"Hell, Rüdiger","family":"Hell"}],"origin":[{"dateIssuedDisp":"August 27, 2008","dateIssuedKey":"2008"}]} | ||
| SRT | |a HAASFLORIAMITOCHONDR2720 | ||