Cover Image

Imaging retinal progenitor lineages in developing zebrafish embryos

In this protocol, we describe how to make and analyze four dimensional (4D) movies of retinal lineage in the zebrafish embryo in vivo. 4D consists of three spatial dimensions (3D) reconstructed from stacks of confocal planes plus one time dimension. Our imaging is performed on transgenic cells that...

Full description

Saved in:
Bibliographic Details
Main Authors: Jusuf, Patricia R. (Author) , Harris, William A. (Author) , Poggi, Lucia (Author)
Format: Article (Journal)
Language:English
Published: 2013
In: Cold Spring Harbor protocols
Year: 2013, Issue: 3, Pages: ?
ISSN:1559-6095
DOI:10.1101/pdb.prot073544
Online Access:Verlag, Volltext: http://dx.doi.org/10.1101/pdb.prot073544
Verlag, Volltext: http://cshprotocols.cshlp.org/content/2013/3/pdb.prot073544
Get full text
Author Notes:Patricia Jusuf, William A. Harris, Lucia Poggi

MARC

LEADER 00000caa a2200000 c 4500
001 1558694196
003 DE-627
005 20220813173433.0
007 cr uuu---uuuuu
008 170517s2013 xx |||||o 00| ||eng c
024 7 |a 10.1101/pdb.prot073544  |2 doi 
035 |a (DE-627)1558694196 
035 |a (DE-576)488694191 
035 |a (DE-599)BSZ488694191 
035 |a (OCoLC)1340975520 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Jusuf, Patricia R.  |e VerfasserIn  |0 (DE-588)1028528876  |0 (DE-627)730869547  |0 (DE-576)376006226  |4 aut 
245 1 0 |a Imaging retinal progenitor lineages in developing zebrafish embryos  |c Patricia Jusuf, William A. Harris, Lucia Poggi 
264 1 |c 2013 
300 |a ? 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 17.05.2017 
520 |a In this protocol, we describe how to make and analyze four dimensional (4D) movies of retinal lineage in the zebrafish embryo in vivo. 4D consists of three spatial dimensions (3D) reconstructed from stacks of confocal planes plus one time dimension. Our imaging is performed on transgenic cells that express fluorescent proteins under the control of cell-specific promoters or on cells that transiently express such reporters in specific retinal cell progenitors. An important aspect of lineage tracing is the ability to follow individual cells as they undergo multiple cell divisions, final migration, and differentiation. This may mean many hours of 4D imaging, requiring that cells be kept healthy and maintained under conditions suitable for normal development. The longest movies we have made are ∼50 h. By analyzing these movies, we can see when a specific cell was born and who its sister was, allowing us to reconstruct its retinal lineages in vivo. 
700 1 |a Harris, William A.  |e VerfasserIn  |0 (DE-588)1132737184  |0 (DE-627)888103085  |0 (DE-576)488989205  |4 aut 
700 1 |a Poggi, Lucia  |e VerfasserIn  |0 (DE-588)1028529872  |0 (DE-627)730870901  |0 (DE-576)376007400  |4 aut 
773 0 8 |i Enthalten in  |t Cold Spring Harbor protocols  |d Cold Spring Harbor, NY : [Verlag nicht ermittelbar], 2006  |g (2013), 3, Seite ?  |h Online-Ressource  |w (DE-627)519199154  |w (DE-600)2254775-7  |w (DE-576)287964558  |x 1559-6095  |7 nnas  |a Imaging retinal progenitor lineages in developing zebrafish embryos 
773 1 8 |g year:2013  |g number:3  |g pages:?  |g extent:?  |a Imaging retinal progenitor lineages in developing zebrafish embryos 
856 4 0 |u http://dx.doi.org/10.1101/pdb.prot073544  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u http://cshprotocols.cshlp.org/content/2013/3/pdb.prot073544  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20170517 
993 |a Article 
994 |a 2013 
998 |g 1028529872  |a Poggi, Lucia  |m 1028529872:Poggi, Lucia  |d 700000  |d 721000  |e 700000PP1028529872  |e 721000PP1028529872  |k 0/700000/  |k 1/700000/721000/  |p 3  |y j 
999 |a KXP-PPN1558694196  |e 2969569442 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"Imaging retinal progenitor lineages in developing zebrafish embryos","title":"Imaging retinal progenitor lineages in developing zebrafish embryos"}],"person":[{"role":"aut","display":"Jusuf, Patricia R.","roleDisplay":"VerfasserIn","given":"Patricia R.","family":"Jusuf"},{"given":"William A.","family":"Harris","role":"aut","roleDisplay":"VerfasserIn","display":"Harris, William A."},{"given":"Lucia","family":"Poggi","role":"aut","display":"Poggi, Lucia","roleDisplay":"VerfasserIn"}],"note":["Gesehen am 17.05.2017"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1558694196","language":["eng"],"origin":[{"dateIssuedDisp":"2013","dateIssuedKey":"2013"}],"id":{"eki":["1558694196"],"doi":["10.1101/pdb.prot073544"]},"name":{"displayForm":["Patricia Jusuf, William A. Harris, Lucia Poggi"]},"physDesc":[{"extent":"? S."}],"relHost":[{"title":[{"title":"Cold Spring Harbor protocols","title_sort":"Cold Spring Harbor protocols"}],"pubHistory":["Nachgewiesen 2006 -"],"part":{"extent":"?","text":"(2013), 3, Seite ?","issue":"3","pages":"?","year":"2013"},"titleAlt":[{"title":"CSH Protocols"}],"disp":"Imaging retinal progenitor lineages in developing zebrafish embryosCold Spring Harbor protocols","type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"519199154","language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"Cold Spring Harbor Laboratory","role":"isb"}],"origin":[{"publisherPlace":"Cold Spring Harbor, NY","dateIssuedKey":"2006","publisher":"[Verlag nicht ermittelbar]","dateIssuedDisp":"2006-"}],"id":{"issn":["1559-6095"],"zdb":["2254775-7"],"eki":["519199154"]},"name":{"displayForm":["Cold Spring Harbor Laboratory"]},"physDesc":[{"extent":"Online-Ressource"}]}]} 
SRT |a JUSUFPATRIIMAGINGRET2013 

Similar Items