Silencing an N-Acyltransferase-Like involved in lignin biosynthesis in nicotiana attenuata dramatically alters herbivory-induced phenolamide metabolism
In a transcriptomic screen of Manduca sexta-induced N-acyltransferases in leaves of Nicotiana attenuata, we identified an N-acyltransferase gene sharing a high similarity with the tobacco lignin-biosynthetic hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) gene whose express...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
May 21, 2013
|
| In: |
PLOS ONE
Year: 2013, Jahrgang: 8, Heft: 5 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0062336 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1371/journal.pone.0062336 Verlag, kostenfrei, Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062336 
|
| Verfasserangaben: | Emmanuel Gaquerel, Hemlata Kotkar, Nawaporn Onkokesung, Ivan Galis, Ian T. Baldwin |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558747192 | ||
| 003 | DE-627 | ||
| 005 | 20220813173913.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170518s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0062336 |2 doi | |
| 035 | |a (DE-627)1558747192 | ||
| 035 | |a (DE-576)488747198 | ||
| 035 | |a (DE-599)BSZ488747198 | ||
| 035 | |a (OCoLC)1340975586 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Gaquerel, Emmanuel |e VerfasserIn |0 (DE-588)1124181636 |0 (DE-627)877879494 |0 (DE-576)482380500 |4 aut | |
| 245 | 1 | 0 | |a Silencing an N-Acyltransferase-Like involved in lignin biosynthesis in nicotiana attenuata dramatically alters herbivory-induced phenolamide metabolism |c Emmanuel Gaquerel, Hemlata Kotkar, Nawaporn Onkokesung, Ivan Galis, Ian T. Baldwin |
| 264 | 1 | |c May 21, 2013 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 18.05.2017 | ||
| 520 | |a In a transcriptomic screen of Manduca sexta-induced N-acyltransferases in leaves of Nicotiana attenuata, we identified an N-acyltransferase gene sharing a high similarity with the tobacco lignin-biosynthetic hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) gene whose expression is controlled by MYB8, a transcription factor that regulates the production of phenylpropanoid polyamine conjugates (phenolamides, PAs). To evaluate the involvement of this HCT-like gene in lignin production as well as the resulting crosstalk with PA metabolism during insect herbivory, we transiently silenced (by VIGs) the expression of this gene and performed non-targeted (UHPLC-ESI/TOF-MS) metabolomics analyses. In agreement with a conserved function of N. attenuata HCT-like in lignin biogenesis, HCT-silenced plants developed weak, soft stems with greatly reduced lignin contents. Metabolic profiling demonstrated large shifts (up to 12% deregulation in total extracted ions in insect-attacked leaves) due to a large diversion of activated coumaric acid units into the production of developmentally and herbivory-induced coumaroyl-containing PAs (N′,N′′-dicoumaroylspermidine, N′,N′′-coumaroylputrescine, etc) and to minor increases in the most abundant free phenolics (chlorogenic and cryptochlorogenic acids), all without altering the production of well characterized herbivory-responsive caffeoyl- and feruloyl-based putrescine and spermidine PAs. These data are consistent with a strong metabolic tension, exacerbated during herbivory, over the allocation of coumaroyl-CoA units among lignin and unusual coumaroyl-containing PAs, and rule out a role for HCT-LIKE in tuning the herbivory-induced accumulation of other PAs. Additionally, these results are consistent with a role for lignification as an induced anti-herbivore defense. | ||
| 650 | 4 | |a Biosynthesis | |
| 650 | 4 | |a Herbivory | |
| 650 | 4 | |a Insects | |
| 650 | 4 | |a Leaves | |
| 650 | 4 | |a Lignin | |
| 650 | 4 | |a Metabolites | |
| 650 | 4 | |a Nicotiana | |
| 650 | 4 | |a Plant-herbivore interactions | |
| 700 | 1 | |a Kotkar, Hemlata M. |e VerfasserIn |0 (DE-588)1132256038 |0 (DE-627)887665888 |0 (DE-576)488746779 |4 aut | |
| 700 | 1 | |a Onkokesung, Nawaporn |d 1979- |e VerfasserIn |0 (DE-588)141425601 |0 (DE-627)627785107 |0 (DE-576)32382661X |4 aut | |
| 700 | 1 | |a Galis, Ivan |e VerfasserIn |0 (DE-588)113189605X |0 (DE-627)887657060 |0 (DE-576)488556430 |4 aut | |
| 700 | 1 | |a Baldwin, Ian T. |d 1958- |e VerfasserIn |0 (DE-588)102710634X |0 (DE-627)728270390 |0 (DE-576)372477844 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 8(2013,5) Artikel-Nummer e62336, 13 Seiten |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a Silencing an N-Acyltransferase-Like involved in lignin biosynthesis in nicotiana attenuata dramatically alters herbivory-induced phenolamide metabolism |
| 773 | 1 | 8 | |g volume:8 |g year:2013 |g number:5 |a Silencing an N-Acyltransferase-Like involved in lignin biosynthesis in nicotiana attenuata dramatically alters herbivory-induced phenolamide metabolism |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.pone.0062336 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062336 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170518 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 1124181636 |a Gaquerel, Emmanuel |m 1124181636:Gaquerel, Emmanuel |p 1 |x j | ||
| 999 | |a KXP-PPN1558747192 |e 2969647974 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 18.05.2017"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","dateIssuedDisp":"2006-","dateIssuedKey":"2006","publisher":"PLOS ; PLoS"}],"id":{"eki":["523574592"],"zdb":["2267670-3"],"issn":["1932-6203"]},"name":{"displayForm":["Public Library of Science"]},"pubHistory":["1.2006 -"],"part":{"issue":"5","year":"2013","text":"8(2013,5) Artikel-Nummer e62336, 13 Seiten","volume":"8"},"note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"disp":"Silencing an N-Acyltransferase-Like involved in lignin biosynthesis in nicotiana attenuata dramatically alters herbivory-induced phenolamide metabolismPLOS ONE","type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"corporate":[{"role":"isb","display":"Public Library of Science","roleDisplay":"Herausgebendes Organ"}],"recId":"523574592","title":[{"title_sort":"PLOS ONE","title":"PLOS ONE"}]}],"recId":"1558747192","person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Gaquerel, Emmanuel","given":"Emmanuel","family":"Gaquerel"},{"family":"Kotkar","given":"Hemlata M.","roleDisplay":"VerfasserIn","display":"Kotkar, Hemlata M.","role":"aut"},{"family":"Onkokesung","given":"Nawaporn","roleDisplay":"VerfasserIn","display":"Onkokesung, Nawaporn","role":"aut"},{"role":"aut","display":"Galis, Ivan","roleDisplay":"VerfasserIn","given":"Ivan","family":"Galis"},{"family":"Baldwin","given":"Ian T.","display":"Baldwin, Ian T.","roleDisplay":"VerfasserIn","role":"aut"}],"name":{"displayForm":["Emmanuel Gaquerel, Hemlata Kotkar, Nawaporn Onkokesung, Ivan Galis, Ian T. Baldwin"]},"origin":[{"dateIssuedDisp":"May 21, 2013","dateIssuedKey":"2013"}],"title":[{"title":"Silencing an N-Acyltransferase-Like involved in lignin biosynthesis in nicotiana attenuata dramatically alters herbivory-induced phenolamide metabolism","title_sort":"Silencing an N-Acyltransferase-Like involved in lignin biosynthesis in nicotiana attenuata dramatically alters herbivory-induced phenolamide metabolism"}],"id":{"eki":["1558747192"],"doi":["10.1371/journal.pone.0062336"]}} | ||
| SRT | |a GAQUERELEMSILENCINGA2120 | ||