Cover Image

Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling

Protein Nα-terminal acetylation represents one of the most abundant protein modifications of higher eukaryotes. In humans, six Nα-acetyltransferases (Nats) are responsible for the acetylation of approximately 80% of the cytosolic proteins. N-terminal protein acetylation has not been evidenced in org...

Full description

Saved in:
Bibliographic Details
Main Authors: Trinh, Dinh-Van (Author) , Linster, Eric (Author) , Feldman-Salit, Anna (Author) , Hell, Rüdiger (Author) , Wirtz, Markus (Author)
Format: Article (Journal)
Language:English
Published: April 30, 2015
In: Proteomics
Year: 2015, Volume: 15, Issue: 14, Pages: 2426-2435
ISSN:1615-9861
DOI:10.1002/pmic.201500025
Online Access:Verlag, Volltext: http://dx.doi.org/10.1002/pmic.201500025
Verlag, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/pmic.201500025/abstract
Get full text
Author Notes:Trinh V. Dinh, Willy V. Bienvenut, Eric Linster, Anna Feldman-Salit, Vincent A. Jung, Thierry Meinnel, Rüdiger Hell, Carmela Giglione and Markus Wirtz

MARC

LEADER 00000caa a2200000 c 4500
001 1558823417
003 DE-627
005 20220813174430.0
007 cr uuu---uuuuu
008 170519s2015 xx |||||o 00| ||eng c
024 7 |a 10.1002/pmic.201500025  |2 doi 
035 |a (DE-627)1558823417 
035 |a (DE-576)488823412 
035 |a (DE-599)BSZ488823412 
035 |a (OCoLC)1340975634 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Trinh, Dinh-Van  |e VerfasserIn  |0 (DE-588)1047903342  |0 (DE-627)779456475  |0 (DE-576)401738272  |4 aut 
245 1 0 |a Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling  |c Trinh V. Dinh, Willy V. Bienvenut, Eric Linster, Anna Feldman-Salit, Vincent A. Jung, Thierry Meinnel, Rüdiger Hell, Carmela Giglione and Markus Wirtz 
264 1 |c April 30, 2015 
300 |a 10 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 19.05.2017 
520 |a Protein Nα-terminal acetylation represents one of the most abundant protein modifications of higher eukaryotes. In humans, six Nα-acetyltransferases (Nats) are responsible for the acetylation of approximately 80% of the cytosolic proteins. N-terminal protein acetylation has not been evidenced in organelles of metazoans, but in higher plants is a widespread modification not only in the cytosol but also in the chloroplast. In this study, we identify and characterize the first organellar-localized Nat in eukaryotes. A primary sequence-based search in Arabidopsis thaliana revealed seven putatively plastid-localized Nats of which AT2G39000 (AtNAA70) showed the highest conservation of the acetyl-CoA binding pocket. The chloroplastic localization of AtNAA70 was demonstrated by transient expression of AtNAA70:YFP in Arabidopsis mesophyll protoplasts. Homology modeling uncovered a significant conservation of tertiary structural elements between human HsNAA50 and AtNAA70. The in vivo acetylation activity of AtNAA70 was demonstrated on a number of distinct protein Nα-termini with a newly established global acetylome profiling test after expression of AtNAA70 in E. coli. AtNAA70 predominately acetylated proteins starting with M, A, S and T, providing an explanation for most protein N-termini acetylation events found in chloroplasts. Like HsNAA50, AtNAA70 displays Nε-acetyltransferase activity on three internal lysine residues. All MS data have been deposited in the ProteomeXchange with identifier PXD001947 (http://proteomecentral.proteomexchange.org/dataset/PXD001947). 
650 4 |a Arabidopsis thaliana 
650 4 |a AtNAA70 
650 4 |a Chloroplast 
650 4 |a Nα-acetyltransferase 
650 4 |a Plant proteomics 
700 1 |a Linster, Eric  |e VerfasserIn  |0 (DE-588)1060573687  |0 (DE-627)800328604  |0 (DE-576)416725953  |4 aut 
700 1 |a Feldman-Salit, Anna  |e VerfasserIn  |0 (DE-588)143189956  |0 (DE-627)643883886  |0 (DE-576)335545262  |4 aut 
700 1 |a Hell, Rüdiger  |e VerfasserIn  |0 (DE-588)1022964933  |0 (DE-627)717340341  |0 (DE-576)166399647  |4 aut 
700 1 |a Wirtz, Markus  |e VerfasserIn  |0 (DE-588)1023118378  |0 (DE-627)717709353  |0 (DE-576)36641979X  |4 aut 
773 0 8 |i Enthalten in  |t Proteomics  |d Weinheim : Wiley VCH, 2001  |g 15(2015), 14, Seite 2426-2435  |h Online-Ressource  |w (DE-627)325573387  |w (DE-600)2037674-1  |w (DE-576)099025825  |x 1615-9861  |7 nnas  |a Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling 
773 1 8 |g volume:15  |g year:2015  |g number:14  |g pages:2426-2435  |g extent:10  |a Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling 
856 4 0 |u http://dx.doi.org/10.1002/pmic.201500025  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u http://onlinelibrary.wiley.com/doi/10.1002/pmic.201500025/abstract  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20170519 
993 |a Article 
994 |a 2015 
998 |g 1023118378  |a Wirtz, Markus  |m 1023118378:Wirtz, Markus  |d 700000  |d 721000  |e 700000PW1023118378  |e 721000PW1023118378  |k 0/700000/  |k 1/700000/721000/  |p 9  |y j 
998 |g 1022964933  |a Hell, Rüdiger  |m 1022964933:Hell, Rüdiger  |d 700000  |d 721000  |e 700000PH1022964933  |e 721000PH1022964933  |k 0/700000/  |k 1/700000/721000/  |p 7 
998 |g 143189956  |a Feldman-Salit, Anna  |m 143189956:Feldman-Salit, Anna  |d 700000  |d 721000  |e 700000PF143189956  |e 721000PF143189956  |k 0/700000/  |k 1/700000/721000/  |p 4 
998 |g 1060573687  |a Linster, Eric  |m 1060573687:Linster, Eric  |d 700000  |d 721000  |e 700000PL1060573687  |e 721000PL1060573687  |k 0/700000/  |k 1/700000/721000/  |p 3 
998 |g 1047903342  |a Trinh, Dinh-Van  |m 1047903342:Trinh, Dinh-Van  |p 1  |x j 
999 |a KXP-PPN1558823417  |e 2969793601 
BIB |a Y 
SER |a journal 
JSO |a {"note":["Gesehen am 19.05.2017"],"relHost":[{"part":{"issue":"14","year":"2015","volume":"15","pages":"2426-2435","text":"15(2015), 14, Seite 2426-2435","extent":"10"},"id":{"zdb":["2037674-1"],"issn":["1615-9861"],"eki":["325573387"],"doi":["10.1002/(ISSN)1615-9861"]},"origin":[{"dateIssuedDisp":"[2001]-","publisher":"Wiley VCH","publisherPlace":"Weinheim"}],"disp":"Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profilingProteomics","recId":"325573387","title":[{"title":"Proteomics","subtitle":"Proteomics and systems biology","title_sort":"Proteomics"}],"pubHistory":["1.2001 -"],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 10.11.2022"]}],"title":[{"title_sort":"Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling","title":"Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling"}],"person":[{"display":"Trinh, Dinh-Van","role":"aut","given":"Dinh-Van","family":"Trinh"},{"family":"Linster","role":"aut","given":"Eric","display":"Linster, Eric"},{"family":"Feldman-Salit","given":"Anna","role":"aut","display":"Feldman-Salit, Anna"},{"display":"Hell, Rüdiger","role":"aut","given":"Rüdiger","family":"Hell"},{"display":"Wirtz, Markus","family":"Wirtz","given":"Markus","role":"aut"}],"physDesc":[{"extent":"10 S."}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"April 30, 2015"}],"id":{"doi":["10.1002/pmic.201500025"],"eki":["1558823417"]},"recId":"1558823417","name":{"displayForm":["Trinh V. Dinh, Willy V. Bienvenut, Eric Linster, Anna Feldman-Salit, Vincent A. Jung, Thierry Meinnel, Rüdiger Hell, Carmela Giglione and Markus Wirtz"]}} 
SRT |a TRINHDINHVMOLECULARI3020 

Similar Items