Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels
Thin hydrogel films based on an ABA triblock copolymer gelator [where A is pH-sensitive poly(2-(diisopropylamino)ethyl methacrylate) (PDPA) and B is biocompatible poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC)] were used as a stimulus-responsive substrate that allows fine adjustment of the...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 10, 2011
|
| In: |
Journal of the American Chemical Society
Year: 2011, Volume: 133, Issue: 5, Pages: 1367-1374 |
| ISSN: | 1520-5126 |
| DOI: | 10.1021/ja1060615 |
| Online Access: | Verlag, kostenfrei registrierungspflichtig, Volltext: http://dx.doi.org/10.1021/ja1060615
|
| Author Notes: | Hiroshi Y. Yoshikawa, Fernanda F. Rossetti, Stefan Kaufmann, Thomas Kaindl, Jeppe Madsen, Ulrike Engel, Andrew L. Lewis, Steven P. Armes, Motomu Tanaka |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1558841474 | ||
| 003 | DE-627 | ||
| 005 | 20230428071127.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170522s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/ja1060615 |2 doi | |
| 035 | |a (DE-627)1558841474 | ||
| 035 | |a (DE-576)48884147X | ||
| 035 | |a (DE-599)BSZ48884147X | ||
| 035 | |a (OCoLC)1340975377 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Yoshikawa, Hiroshi |e VerfasserIn |0 (DE-588)1132516811 |0 (DE-627)887837336 |0 (DE-576)488839009 |4 aut | |
| 245 | 1 | 0 | |a Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels |c Hiroshi Y. Yoshikawa, Fernanda F. Rossetti, Stefan Kaufmann, Thomas Kaindl, Jeppe Madsen, Ulrike Engel, Andrew L. Lewis, Steven P. Armes, Motomu Tanaka |
| 264 | 1 | |c January 10, 2011 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.05.2017 | ||
| 520 | |a Thin hydrogel films based on an ABA triblock copolymer gelator [where A is pH-sensitive poly(2-(diisopropylamino)ethyl methacrylate) (PDPA) and B is biocompatible poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC)] were used as a stimulus-responsive substrate that allows fine adjustment of the mechanical environment experienced by mouse myoblast cells. The hydrogel film elasticity could be reversibly modulated by a factor of 40 via careful pH adjustment without adversely affecting cell viability. Myoblast cells exhibited pronounced stress fiber formation and flattening on increasing the hydrogel elasticity. As a new tool to evaluate the strength of cell adhesion, we combined a picosecond laser with an inverted microscope and utilized the strong shock wave created by the laser pulse to determine the critical pressure required for cell detachment. Furthermore, we demonstrate that an abrupt jump in the hydrogel elasticity can be utilized to monitor how cells adapt their morphology to changes in their mechanical environment. | ||
| 700 | 1 | |a Rossetti, Fernanda |e VerfasserIn |0 (DE-588)1132518741 |0 (DE-627)887839223 |0 (DE-576)488840732 |4 aut | |
| 700 | 1 | |a Kaufmann, Stefan |d 1948- |e VerfasserIn |0 (DE-588)1060647400 |0 (DE-627)800412656 |0 (DE-576)416771424 |4 aut | |
| 700 | 1 | |a Kaindl, Thomas |e VerfasserIn |0 (DE-588)143675915 |0 (DE-627)653845820 |0 (DE-576)338318976 |4 aut | |
| 700 | 1 | |a Engel, Ulrike |e VerfasserIn |0 (DE-588)1123163480 |0 (DE-627)879999721 |0 (DE-576)48164525X |4 aut | |
| 700 | 1 | |a Tanaka, Motomu |e VerfasserIn |0 (DE-588)1060648377 |0 (DE-627)800414829 |0 (DE-576)416774636 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a American Chemical Society |t Journal of the American Chemical Society |d Washington, DC : ACS Publications, 1879 |g 133(2011), 5, Seite 1367-1374 |w (DE-627)268132836 |w (DE-600)1472210-0 |w (DE-576)090854284 |x 1520-5126 |7 nnas |
| 773 | 1 | 8 | |g volume:133 |g year:2011 |g number:5 |g pages:1367-1374 |g extent:8 |a Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/ja1060615 |x Verlag |x Resolving-System |z kostenfrei registrierungspflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170522 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 1060648377 |a Tanaka, Motomu |m 1060648377:Tanaka, Motomu |d 120000 |d 120300 |e 120000PT1060648377 |e 120300PT1060648377 |k 0/120000/ |k 1/120000/120300/ |p 9 |y j | ||
| 998 | |g 1123163480 |a Engel, Ulrike |m 1123163480:Engel, Ulrike |d 700000 |d 716000 |e 700000PE1123163480 |e 716000PE1123163480 |k 0/700000/ |k 1/700000/716000/ |p 6 | ||
| 998 | |g 143675915 |a Kaindl, Thomas |m 143675915:Kaindl, Thomas |d 120000 |d 120300 |e 120000PK143675915 |e 120300PK143675915 |k 0/120000/ |k 1/120000/120300/ |p 4 | ||
| 998 | |g 1060647400 |a Kaufmann, Stefan |m 1060647400:Kaufmann, Stefan |d 120000 |d 120300 |e 120000PK1060647400 |e 120300PK1060647400 |k 0/120000/ |k 1/120000/120300/ |p 3 | ||
| 998 | |g 1132518741 |a Rossetti, Fernanda |m 1132518741:Rossetti, Fernanda |d 120000 |d 120300 |e 120000PR1132518741 |e 120300PR1132518741 |k 0/120000/ |k 1/120000/120300/ |p 2 | ||
| 998 | |g 1132516811 |a Yoshikawa, Hiroshi |m 1132516811:Yoshikawa, Hiroshi |d 120000 |d 120300 |e 120000PY1132516811 |e 120300PY1132516811 |k 0/120000/ |k 1/120000/120300/ |p 1 |x j | ||
| 999 | |a KXP-PPN1558841474 |e 2969856867 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"disp":"American Chemical SocietyJournal of the American Chemical Society","corporate":[{"display":"American Chemical Society","role":"aut"}],"language":["eng"],"part":{"year":"2011","pages":"1367-1374","text":"133(2011), 5, Seite 1367-1374","extent":"8","volume":"133","issue":"5"},"note":["Gesehen am 25.08.2020"],"origin":[{"dateIssuedDisp":"1879-","publisherPlace":"Washington, DC ; Washington, DC","publisher":"ACS Publications ; American Chemical Society","dateIssuedKey":"1879"}],"titleAlt":[{"title":"JACS"},{"title":"web edition"}],"title":[{"subtitle":"JACS","title_sort":"Journal of the American Chemical Society","title":"Journal of the American Chemical Society"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"268132836","pubHistory":["1.1879 -"],"name":{"displayForm":["Ed.: Allen J. Bard [u.a.]"]},"id":{"issn":["1520-5126"],"eki":["268132836"],"zdb":["1472210-0"]}}],"id":{"doi":["10.1021/ja1060615"],"eki":["1558841474"]},"name":{"displayForm":["Hiroshi Y. Yoshikawa, Fernanda F. Rossetti, Stefan Kaufmann, Thomas Kaindl, Jeppe Madsen, Ulrike Engel, Andrew L. Lewis, Steven P. Armes, Motomu Tanaka"]},"physDesc":[{"extent":"8 S."}],"recId":"1558841474","note":["Gesehen am 22.05.2017"],"title":[{"title_sort":"Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels","title":"Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels"}],"origin":[{"dateIssuedDisp":"January 10, 2011","dateIssuedKey":"2011"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"person":[{"family":"Yoshikawa","display":"Yoshikawa, Hiroshi","given":"Hiroshi","role":"aut"},{"role":"aut","given":"Fernanda","display":"Rossetti, Fernanda","family":"Rossetti"},{"display":"Kaufmann, Stefan","family":"Kaufmann","role":"aut","given":"Stefan"},{"role":"aut","given":"Thomas","family":"Kaindl","display":"Kaindl, Thomas"},{"family":"Engel","display":"Engel, Ulrike","given":"Ulrike","role":"aut"},{"role":"aut","given":"Motomu","family":"Tanaka","display":"Tanaka, Motomu"}]} | ||
| SRT | |a YOSHIKAWAHQUANTITATI1020 | ||