Live imaging of active fluorophore labelled Wnt proteins
Here, we have generated a Wnt2b-EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b-EGFP moves along the microtubules of Wnt producing cells and that th...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
26 April 2012
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| In: |
FEBS letters
Year: 2012, Volume: 586, Issue: 11, Pages: 1638-1644 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2012.04.035 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.febslet.2012.04.035 Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2012.04.035/abstract 
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| Author Notes: | Tatjana Holzer, Katrin Liffers, Karolin Rahm, Benjamin Trageser, Suat Özbek, Dietmar Gradl |
| Summary: | Here, we have generated a Wnt2b-EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b-EGFP moves along the microtubules of Wnt producing cells and that this directed movement is essential for the secretion of active Wnt protein. |
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| Item Description: | Gesehen am 24.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2012.04.035 |