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Live imaging of active fluorophore labelled Wnt proteins

Here, we have generated a Wnt2b-EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b-EGFP moves along the microtubules of Wnt producing cells and that th...

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Bibliographische Detailangaben
Hauptverfasser: Holzer, Tatjana (VerfasserIn) , Trageser, Benjamin (VerfasserIn) , Özbek, Suat (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 26 April 2012
In: FEBS letters
Year: 2012, Jahrgang: 586, Heft: 11, Pages: 1638-1644
ISSN:1873-3468
DOI:10.1016/j.febslet.2012.04.035
Online-Zugang:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.febslet.2012.04.035
Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2012.04.035/abstract
Volltext
Verfasserangaben:Tatjana Holzer, Katrin Liffers, Karolin Rahm, Benjamin Trageser, Suat Özbek, Dietmar Gradl
Beschreibung
Zusammenfassung:Here, we have generated a Wnt2b-EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b-EGFP moves along the microtubules of Wnt producing cells and that this directed movement is essential for the secretion of active Wnt protein.
Beschreibung:Gesehen am 24.05.2017
Beschreibung:Online Resource
ISSN:1873-3468
DOI:10.1016/j.febslet.2012.04.035

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