Single-cell-based system to monitor carrier driven cellular auxin homeostasis
Abundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular auxin homeostasis. Despite the developmental importance o...
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| Main Authors: | , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
4 February 2013
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| In: |
BMC plant biology
Year: 2013, Volume: 13 |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/1471-2229-13-20 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1186/1471-2229-13-20
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| Author Notes: | Elke Barbez, Martina Laňková, Markéta Pařezová, Alexis Maizel, Eva Zažímalová, Jan Petrášek, Jiří Friml and Jürgen Kleine-Vehn |
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| 520 | |a Abundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular auxin homeostasis. Despite the developmental importance of auxin transporters, a simple medium-to-high throughput approach to assess carrier activities is still missing. Here we show that carrier driven depletion of cellular auxin correlates with reduced nuclear auxin signaling in tobacco Bright Yellow-2 (BY-2) cell cultures. | ||
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