Live cell imaging with R-GECO1 sheds light on flg22- and Chitin-induced transient [Ca2+]cyt patterns in Arabidopsis
Intracellular Ca2+ transients are an integral part of the signaling cascade during pathogen-associated molecular pattern (PAMP)-triggered immunity in plants. Yet, our knowledge about the spatial distribution of PAMP-induced Ca2+ signals is limited. Investigation of cell- and tissue-specific properti...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
19 May 2015
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| In: |
Molecular plant
Year: 2015, Jahrgang: 8, Heft: 8, Pages: 1188-1200 |
| ISSN: | 1752-9867 |
| DOI: | 10.1016/j.molp.2015.05.006 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.molp.2015.05.006 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1674205215002397 
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| Verfasserangaben: | Nana F. Keinath, Rainer Waadt, Rik Brugman, Julian I. Schroeder, Guido Grossmann, Karin Schumacher, Melanie Krebs |
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| 520 | |a Intracellular Ca2+ transients are an integral part of the signaling cascade during pathogen-associated molecular pattern (PAMP)-triggered immunity in plants. Yet, our knowledge about the spatial distribution of PAMP-induced Ca2+ signals is limited. Investigation of cell- and tissue-specific properties of Ca2+-dependent signaling processes requires versatile Ca2+ reporters that are able to extract spatial information from cellular and subcellular structures, as well as from whole tissues over time periods from seconds to hours. Fluorescence-based reporters cover both a broad spatial and temporal range, which makes them ideally suited to study Ca2+ signaling in living cells. In this study, we compared two fluorescence-based Ca2+ sensors: the Förster resonance energy transfer (FRET)-based reporter yellow cameleon NES-YC3.6 and the intensity-based sensor R-GECO1. We demonstrate that R-GECO1 exhibits a significantly increased signal change compared with ratiometric NES-YC3.6 in response to several stimuli. Due to its superior sensitivity, R-GECO1 is able to report flg22- and chitin-induced Ca2+ signals on a cellular scale, which allowed identification of defined [Ca2+]cyt oscillations in epidermal and guard cells in response to the fungal elicitor chitin. Moreover, we discovered that flg22- and chitin-induced Ca2+ signals in the root initiate from the elongation zone. | ||
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