Effects of low seawater pH on the marine polychaete Platynereis dumerilii
An important priority for any organism is to maintain internal cellular homeostasis including acid-base balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA),...
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| Hauptverfasser: | , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 June 2015
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| In: |
Marine pollution bulletin
Year: 2015, Jahrgang: 95, Heft: 1, Pages: 166-172 |
| ISSN: | 1879-3363 |
| DOI: | 10.1016/j.marpolbul.2015.04.027 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.marpolbul.2015.04.027 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0025326X15002295 |
| Verfasserangaben: | Janine Wäge, Jörg D. Hardege, Tomas A. Larsson, Oleg Simakov, Emma C. Chapman, Detlev Arendt, Jeanette M. Rotchell |
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| 245 | 1 | 0 | |a Effects of low seawater pH on the marine polychaete Platynereis dumerilii |c Janine Wäge, Jörg D. Hardege, Tomas A. Larsson, Oleg Simakov, Emma C. Chapman, Detlev Arendt, Jeanette M. Rotchell |
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| 520 | |a An important priority for any organism is to maintain internal cellular homeostasis including acid-base balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA), and calmodulin (CaM) genes from a polychaete, Platynereis dumerilii and investigate their relative expression in acidified seawater conditions. mRNA expression of NHE was significantly down-regulated after 1h and up-regulated after 7days under low pH treatment (pH 7.8), indicating changes in acid-base transport. Furthermore, the localisation of NHE expression was also altered. A trend of down regulation in CA after 1h was also observed, suggesting a shift in the CO2 and HCO3− balance. No change in CaM expression was detected after 7days exposure to acidified seawater. This study provides insight into the molecular level changes taking place following exposure to acidified seawater in a non-calcifying, ubiquitous, organism. | ||
| 650 | 4 | |a Acid-base regulation | |
| 650 | 4 | |a Gene expression | |
| 650 | 4 | |a Low pH | |
| 650 | 4 | |a Polychaeta | |
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