Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system
The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This s...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
22 April 2010
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| In: |
Genesis
Year: 2010, Volume: 48, Issue: 7, Pages: 452-456 |
| ISSN: | 1526-968X |
| DOI: | 10.1002/dvg.20637 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1002/dvg.20637 Verlag, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/dvg.20637/abstract Verlag, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/dvg.20637/epdf 
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| Author Notes: | Aurelia L. Boy, Zongzhao Zhai, Anette Habring-Müller, Yvonne Kussler-Schneider, Petra Kaspar, Ingrid Lohmann |
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| 520 | |a The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452-456, 2010. © 2010 Wiley-Liss, Inc. | ||
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