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Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system

The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This s...

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Bibliographic Details
Main Authors: Boy, Aurelia Lara (Author) , Zhai, Zongzhao (Author) , Kussler-Schneider, Yvonne (Author) , Kaspar, Petra (Author) , Lohmann, Ingrid (Author)
Format: Article (Journal)
Language:English
Published: 22 April 2010
In: Genesis
Year: 2010, Volume: 48, Issue: 7, Pages: 452-456
ISSN:1526-968X
DOI:10.1002/dvg.20637
Online Access:Verlag, Volltext: http://dx.doi.org/10.1002/dvg.20637
Verlag, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/dvg.20637/abstract
Verlag, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/dvg.20637/epdf
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Author Notes:Aurelia L. Boy, Zongzhao Zhai, Anette Habring-Müller, Yvonne Kussler-Schneider, Petra Kaspar, Ingrid Lohmann
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Summary:The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452-456, 2010. © 2010 Wiley-Liss, Inc.
Item Description:Gesehen am 13.06.2017
Physical Description:Online Resource
ISSN:1526-968X
DOI:10.1002/dvg.20637

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