Germline-transmitted genome editing in arabidopsis thaliana using TAL-effector-nucleases
Transcription activator-like effector nucleases (TALENs) are custom-made bi-partite endonucleases that have recently been developed and applied for genome engineering in a wide variety of organisms. However, they have been only scarcely used in plants, especially for germline-modification. Here we r...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
March 30, 2015
|
| In: |
PLOS ONE
Year: 2015, Jahrgang: 10, Heft: 3 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0121056 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1371/journal.pone.0121056 Verlag, kostenfrei, Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121056 Verlag, kostenfrei, Volltext: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0121056&type=printable 
|
| Verfasserangaben: | Joachim Forner, Anne Pfeiffer, Tobias Langenecker, Pablo Manavella, Jan U. Lohmann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1559831898 | ||
| 003 | DE-627 | ||
| 005 | 20220813183027.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170619s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0121056 |2 doi | |
| 035 | |a (DE-627)1559831898 | ||
| 035 | |a (DE-576)489831893 | ||
| 035 | |a (DE-599)BSZ489831893 | ||
| 035 | |a (OCoLC)1340975882 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Forner, Joachim |e VerfasserIn |0 (DE-588)133153029 |0 (DE-627)537510117 |0 (DE-576)186492936 |4 aut | |
| 245 | 1 | 0 | |a Germline-transmitted genome editing in arabidopsis thaliana using TAL-effector-nucleases |c Joachim Forner, Anne Pfeiffer, Tobias Langenecker, Pablo Manavella, Jan U. Lohmann |
| 264 | 1 | |c March 30, 2015 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 19.06.2017 | ||
| 500 | |a Eine Namenskorrektur des 4. Verfassers (Manavella, Pablo) ist unter der DOI https://doi.org/10.1371/journal.pone.0133945 zu finden | ||
| 520 | |a Transcription activator-like effector nucleases (TALENs) are custom-made bi-partite endonucleases that have recently been developed and applied for genome engineering in a wide variety of organisms. However, they have been only scarcely used in plants, especially for germline-modification. Here we report the efficient creation of small, germline-transmitted deletions in Arabidopsis thaliana via TALENs that were delivered by stably integrated transgenes. Using meristem specific promoters to drive expression of two TALEN arms directed at the CLV3 coding sequence, we observed very high phenotype frequencies in the T2 generation. In some instances, full CLV3 loss-of-function was already observed in the T1 generation, suggesting that transgenic delivery of TALENs can cause highly efficient genome modification. In contrast, constitutive TALEN expression in the shoot apical meristem (SAM) did not cause additional phenotypes and genome re-sequencing confirmed little off-target effects, demonstrating exquisite target specificity. | ||
| 650 | 4 | |a Arabidopsis thaliana | |
| 650 | 4 | |a Flowering plants | |
| 650 | 4 | |a Genetically modified plants | |
| 650 | 4 | |a Genetic loci | |
| 650 | 4 | |a Meristems | |
| 650 | 4 | |a Phenotypes | |
| 650 | 4 | |a Plant genomics | |
| 650 | 4 | |a TALENs | |
| 700 | 1 | |a Pfeiffer, Anne |e VerfasserIn |0 (DE-588)142613738 |0 (DE-627)638041309 |0 (DE-576)332371557 |4 aut | |
| 700 | 1 | |a Lohmann, Jan U. |d 1971- |e VerfasserIn |0 (DE-588)121572315 |0 (DE-627)70554642X |0 (DE-576)292778627 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 10(2015,3) Artikel-Nummer e121056, 15 Seiten |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a Germline-transmitted genome editing in arabidopsis thaliana using TAL-effector-nucleases |
| 773 | 1 | 8 | |g volume:10 |g year:2015 |g number:3 |g extent:15 |a Germline-transmitted genome editing in arabidopsis thaliana using TAL-effector-nucleases |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.pone.0121056 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121056 |x Verlag |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0121056&type=printable |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170619 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 121572315 |a Lohmann, Jan U. |m 121572315:Lohmann, Jan U. |d 700000 |d 721000 |e 700000PL121572315 |e 721000PL121572315 |k 0/700000/ |k 1/700000/721000/ |p 5 |y j | ||
| 998 | |g 142613738 |a Pfeiffer, Anne |m 142613738:Pfeiffer, Anne |d 700000 |d 721000 |e 700000PP142613738 |e 721000PP142613738 |k 0/700000/ |k 1/700000/721000/ |p 2 | ||
| 998 | |g 133153029 |a Forner, Joachim |m 133153029:Forner, Joachim |d 700000 |d 721000 |e 700000PF133153029 |e 721000PF133153029 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1559831898 |e 297204374X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1559831898","language":["eng"],"note":["Gesehen am 19.06.2017","Eine Namenskorrektur des 4. Verfassers (Manavella, Pablo) ist unter der DOI https://doi.org/10.1371/journal.pone.0133945 zu finden"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title":"Germline-transmitted genome editing in arabidopsis thaliana using TAL-effector-nucleases","title_sort":"Germline-transmitted genome editing in arabidopsis thaliana using TAL-effector-nucleases"}],"person":[{"family":"Forner","given":"Joachim","display":"Forner, Joachim","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Pfeiffer, Anne","role":"aut","family":"Pfeiffer","given":"Anne"},{"role":"aut","display":"Lohmann, Jan U.","roleDisplay":"VerfasserIn","given":"Jan U.","family":"Lohmann"}],"relHost":[{"origin":[{"publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","publisher":"PLOS ; PLoS","dateIssuedKey":"2006","dateIssuedDisp":"2006-"}],"id":{"zdb":["2267670-3"],"eki":["523574592"],"issn":["1932-6203"]},"name":{"displayForm":["Public Library of Science"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"PLOS ONE","title":"PLOS ONE"}],"pubHistory":["1.2006 -"],"part":{"year":"2015","issue":"3","volume":"10","text":"10(2015,3) Artikel-Nummer e121056, 15 Seiten","extent":"15"},"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Germline-transmitted genome editing in arabidopsis thaliana using TAL-effector-nucleasesPLOS ONE","note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"recId":"523574592","language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"Public Library of Science","role":"isb"}]}],"physDesc":[{"extent":"15 S."}],"id":{"doi":["10.1371/journal.pone.0121056"],"eki":["1559831898"]},"origin":[{"dateIssuedDisp":"March 30, 2015","dateIssuedKey":"2015"}],"name":{"displayForm":["Joachim Forner, Anne Pfeiffer, Tobias Langenecker, Pablo Manavella, Jan U. Lohmann"]}} | ||
| SRT | |a FORNERJOACGERMLINETR3020 | ||