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Monoclonal antibody detects oligodendroglial cell surface protein exhibiting temporal regulation during development

As tools to study stage-specific surface molecules expressed during the development of oligodendrocytes, we have generated monoclonal antibodies against peanut agglutinin (PNA)-binding glycoproteins isolated by affinity chromatography from the oligodendroglial precursor cell line Oli-neu. In this pa...

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Bibliographische Detailangaben
Hauptverfasser: Grzenkowski, Martina (VerfasserIn) , Niehaus, Antje (VerfasserIn) , Trotter, Jacqueline (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 22 October 1999
In: Glia
Year: 1999, Jahrgang: 28, Heft: 2, Pages: 128-137$p10
ISSN:1098-1136
DOI:10.1002/(SICI)1098-1136(199911)28:2<128::AID-GLIA4>3.0.CO;2-E
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1002/(SICI)1098-1136(199911)28:2<128::AID-GLIA4>3.0.CO;2-E
Verlag, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-1136(199911)28:2<128::AID-GLIA4>3.0.CO;2-E/abstract
Volltext
Verfasserangaben:Martina Grzenkowski, Antje Niehaus, Jacqueline Trotter
Beschreibung
Zusammenfassung:As tools to study stage-specific surface molecules expressed during the development of oligodendrocytes, we have generated monoclonal antibodies against peanut agglutinin (PNA)-binding glycoproteins isolated by affinity chromatography from the oligodendroglial precursor cell line Oli-neu. In this paper we report the characterization of the monoclonal antibody 7D10. The 7D10 antibody recognizes a 145-kD cell surface glycoprotein expressed by postmitotic multibranched cells of the oligodendroglial lineage. The antigen stains subpopulations of myelin-associated glycoprotein (MAG) and O4-positive cells and is subsequently down-regulated during further differentiation in vitro. The 7D10 antigen is also expressed by a subpopulation of astroglial cells but not by neurons. A truncated form of the protein is released by antigen-expressing cells into the culture supernatant.
Beschreibung:Gesehen am 19.06.2017
Beschreibung:Online Resource
ISSN:1098-1136
DOI:10.1002/(SICI)1098-1136(199911)28:2<128::AID-GLIA4>3.0.CO;2-E

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