Mapping the O-Mannose glycoproteome in saccharomyces cerevisiae
O-Mannosylation is a vital protein modification conserved from fungi to humans. Yeast is a perfect model to study this post-translational modification, because in contrast to mammals O-mannosylation is the only type of O-glycosylation. In an essential step toward the full understanding of protein O-...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
January 13, 2016
|
| In: |
Molecular & cellular proteomics
Year: 2016, Jahrgang: 15, Heft: 4, Pages: 1323-1337 |
| ISSN: | 1535-9484 |
| DOI: | 10.1074/mcp.M115.057505 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1074/mcp.M115.057505 Verlag, kostenfrei, Volltext: http://www.mcponline.org/content/15/4/1323 
|
| Verfasserangaben: | Patrick Neubert, Adnan Halim, Martin Zauser, Andreas Essig, Hiren J. Joshi, Ewa Zatorska, Ida Signe Bohse Larsen, Martin Loibl, Joan Castells-Ballester, Markus Aebi, Henrik Clausen, and Sabine Strahl |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1559873396 | ||
| 003 | DE-627 | ||
| 005 | 20220813183145.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170619s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1074/mcp.M115.057505 |2 doi | |
| 035 | |a (DE-627)1559873396 | ||
| 035 | |a (DE-576)489873391 | ||
| 035 | |a (DE-599)BSZ489873391 | ||
| 035 | |a (OCoLC)1340976187 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Neubert, Patrick |e VerfasserIn |0 (DE-588)1105580997 |0 (DE-627)862698707 |0 (DE-576)47362656X |4 aut | |
| 245 | 1 | 0 | |a Mapping the O-Mannose glycoproteome in saccharomyces cerevisiae |c Patrick Neubert, Adnan Halim, Martin Zauser, Andreas Essig, Hiren J. Joshi, Ewa Zatorska, Ida Signe Bohse Larsen, Martin Loibl, Joan Castells-Ballester, Markus Aebi, Henrik Clausen, and Sabine Strahl |
| 264 | 1 | |c January 13, 2016 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 19.06.2017 | ||
| 520 | |a O-Mannosylation is a vital protein modification conserved from fungi to humans. Yeast is a perfect model to study this post-translational modification, because in contrast to mammals O-mannosylation is the only type of O-glycosylation. In an essential step toward the full understanding of protein O-mannosylation we mapped the O-mannose glycoproteome in baker's yeast. Taking advantage of an O-glycan elongation deficient yeast strain to simplify sample complexity, we identified over 500 O-glycoproteins from all subcellular compartments for which over 2300 O-mannosylation sites were mapped by electron-transfer dissociation (ETD)-based MS/MS. In this study, we focus on the 293 O-glycoproteins (over 1900 glycosylation sites identified by ETD-MS/MS) that enter the secretory pathway and are targets of ER-localized protein O-mannosyltransferases. We find that O-mannosylation is not only a prominent modification of cell wall and plasma membrane proteins, but also of a large number of proteins from the secretory pathway with crucial functions in protein glycosylation, folding, quality control, and trafficking. The analysis of glycosylation sites revealed that O-mannosylation is favored in unstructured regions and β-strands. Furthermore, O-mannosylation is impeded in the proximity of N-glycosylation sites suggesting the interplay of these types of post-translational modifications. The detailed knowledge of the target proteins and their O-mannosylation sites opens for discovery of new roles of this essential modification in eukaryotes, and for a first glance on the evolution of different types of O-glycosylation from yeast to mammals. | ||
| 700 | 1 | |a Zatorska, Ewa |e VerfasserIn |0 (DE-588)1135603057 |0 (DE-627)890522359 |0 (DE-576)48987309X |4 aut | |
| 700 | 1 | |a Loibl, Martin |e VerfasserIn |0 (DE-588)1032260092 |0 (DE-627)738177253 |0 (DE-576)379918676 |4 aut | |
| 700 | 1 | |a Castells Ballester, Joan |d 1990- |e VerfasserIn |0 (DE-588)1135603111 |0 (DE-627)890522510 |0 (DE-576)489873367 |4 aut | |
| 700 | 1 | |a Strahl, Sabine |e VerfasserIn |0 (DE-588)1034793055 |0 (DE-627)74629073X |0 (DE-576)382427939 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular & cellular proteomics |d Bethesda, Md. : The American Society for Biochemistry and Molecular Biology, 2002 |g 15(2016), 4, Seite 1323-1337 |h Online-Ressource |w (DE-627)342318276 |w (DE-600)2071375-7 |w (DE-576)264424549 |x 1535-9484 |7 nnas |a Mapping the O-Mannose glycoproteome in saccharomyces cerevisiae |
| 773 | 1 | 8 | |g volume:15 |g year:2016 |g number:4 |g pages:1323-1337 |g extent:15 |a Mapping the O-Mannose glycoproteome in saccharomyces cerevisiae |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1074/mcp.M115.057505 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://www.mcponline.org/content/15/4/1323 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170619 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 1034793055 |a Strahl, Sabine |m 1034793055:Strahl, Sabine |d 700000 |d 721000 |e 700000PS1034793055 |e 721000PS1034793055 |k 0/700000/ |k 1/700000/721000/ |p 12 |y j | ||
| 998 | |g 1135603111 |a Castells Ballester, Joan |m 1135603111:Castells Ballester, Joan |p 9 | ||
| 998 | |g 1032260092 |a Loibl, Martin |m 1032260092:Loibl, Martin |p 8 | ||
| 998 | |g 1135603057 |a Zatorska, Ewa |m 1135603057:Zatorska, Ewa |d 700000 |d 721000 |e 700000PZ1135603057 |e 721000PZ1135603057 |k 0/700000/ |k 1/700000/721000/ |p 6 | ||
| 998 | |g 1105580997 |a Neubert, Patrick |m 1105580997:Neubert, Patrick |d 700000 |d 721000 |e 700000PN1105580997 |e 721000PN1105580997 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1559873396 |e 2972129997 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Mapping the O-Mannose glycoproteome in saccharomyces cerevisiae","title_sort":"Mapping the O-Mannose glycoproteome in saccharomyces cerevisiae"}],"language":["eng"],"person":[{"display":"Neubert, Patrick","given":"Patrick","family":"Neubert","role":"aut"},{"family":"Zatorska","given":"Ewa","display":"Zatorska, Ewa","role":"aut"},{"family":"Loibl","given":"Martin","display":"Loibl, Martin","role":"aut"},{"display":"Castells Ballester, Joan","given":"Joan","family":"Castells Ballester","role":"aut"},{"given":"Sabine","display":"Strahl, Sabine","family":"Strahl","role":"aut"}],"name":{"displayForm":["Patrick Neubert, Adnan Halim, Martin Zauser, Andreas Essig, Hiren J. Joshi, Ewa Zatorska, Ida Signe Bohse Larsen, Martin Loibl, Joan Castells-Ballester, Markus Aebi, Henrik Clausen, and Sabine Strahl"]},"origin":[{"dateIssuedDisp":"January 13, 2016","dateIssuedKey":"2016"}],"recId":"1559873396","note":["Gesehen am 19.06.2017"],"relHost":[{"type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["1.2002 -"],"language":["eng"],"titleAlt":[{"title":"MCP"},{"title":"Molecular and cellular proteomics"}],"disp":"Mapping the O-Mannose glycoproteome in saccharomyces cerevisiaeMolecular & cellular proteomics","recId":"342318276","note":["Gesehen am 23.05.25"],"physDesc":[{"extent":"Online-Ressource"}],"part":{"volume":"15","text":"15(2016), 4, Seite 1323-1337","pages":"1323-1337","year":"2016","issue":"4","extent":"15"},"id":{"eki":["342318276"],"issn":["1535-9484"],"zdb":["2071375-7"]},"title":[{"title":"Molecular & cellular proteomics","title_sort":"Molecular & cellular proteomics","subtitle":"MCP"}],"origin":[{"publisher":"The American Society for Biochemistry and Molecular Biology","dateIssuedDisp":"2002-","dateIssuedKey":"2002","publisherPlace":"Bethesda, Md."}],"name":{"displayForm":["The American Society for Biochemistry and Molecular Biology (ASBMB)"]}}],"physDesc":[{"extent":"15 S."}],"id":{"doi":["10.1074/mcp.M115.057505"],"eki":["1559873396"]},"type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a NEUBERTPATMAPPINGTHE1320 | ||