Differentially expressed small RNAs in arabidopsis galls formed by meloidogyne javanica: a functional role for miR390 and its TAS3-derived tasiRNAs
Root-knot nematodes (RKNs) induce inside the vascular cylinder the giant cells (GCs) embedded in the galls. The distinctive gene repression in early-developing GCs could be facilitated by small RNAs (sRNA) such as miRNAs, and/or epigenetic mechanisms mediated by 24nt-sRNAs, rasiRNAs and 21-22nt-sRNA...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
3 February 2016
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| In: |
The new phytologist
Year: 2016, Jahrgang: 209, Heft: 4, Pages: 1625-1640 |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.13735 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1111/nph.13735 Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1111/nph.13735/abstract Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1111/nph.13735/epdf 
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| Verfasserangaben: | Javier Cabrera, Marta Barcala, Alejandra García, Ana Rio-Machín, Clémence Medina, Stephanie Jaubert-Possamai, Bruno Favery, Alexis Maizel, Virginia Ruiz-Ferrer, Carmen Fenoll, Carolina Escobar |
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| 245 | 1 | 0 | |a Differentially expressed small RNAs in arabidopsis galls formed by meloidogyne javanica |b a functional role for miR390 and its TAS3-derived tasiRNAs |c Javier Cabrera, Marta Barcala, Alejandra García, Ana Rio-Machín, Clémence Medina, Stephanie Jaubert-Possamai, Bruno Favery, Alexis Maizel, Virginia Ruiz-Ferrer, Carmen Fenoll, Carolina Escobar |
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| 520 | |a Root-knot nematodes (RKNs) induce inside the vascular cylinder the giant cells (GCs) embedded in the galls. The distinctive gene repression in early-developing GCs could be facilitated by small RNAs (sRNA) such as miRNAs, and/or epigenetic mechanisms mediated by 24nt-sRNAs, rasiRNAs and 21-22nt-sRNAs. Therefore, the sRNA-population together with the role of the miR390/TAS3/ARFs module were studied during early gall/GC formation. Three sRNA libraries from 3-d-post-inoculation (dpi) galls induced by Meloidogyne javanica in Arabidopsis and three from uninfected root segments were sequenced following Illumina-Solexa technology. pMIR390a::GUS and pTAS3::GUS lines were assayed for nematode-dependent promoter activation. A sensor line indicative of TAS3-derived tasiRNAs binding to the ARF3 sequence (pARF3:ARF3-GUS) together with a tasiRNA-resistant ARF3 line (pARF3:ARF3m-GUS) were used for functional analysis. The sRNA population showed significant differences between galls and controls, with high validation rate and correspondence with their target expression: 21-nt sRNAs corresponding mainly to miRNAs were downregulated, whilst 24-nt-sRNAs from the rasiRNA family were mostly upregulated in galls. The promoters of MIR390a and TAS3, active in galls, and the pARF3:ARF3-GUS line, indicated a role of TAS3-derived-tasiRNAs in galls. The regulatory module miR390/TAS3 is necessary for proper gall formation possibly through auxin-responsive factors, and the abundance of 24-nt sRNAs (mostly rasiRNAs) constitutes a gall hallmark. | ||
| 650 | 4 | |a galls | |
| 650 | 4 | |a giant cells | |
| 650 | 4 | |a meloidogyne | |
| 650 | 4 | |a miRNAs | |
| 650 | 4 | |a rasiRNAs | |
| 650 | 4 | |a silencing | |
| 650 | 4 | |a small RNAs | |
| 650 | 4 | |a tasiRNAs | |
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