Cover Image

Chk1, but not Chk2, inhibits Cdc25 phosphatases by a novel common mechanism

Cdc25 phosphatases activate cyclin‐dependent kinases (Cdks) and thereby promote cell cycle progression. In vertebrates, Chk1 and Chk2 phosphorylate Cdc25A at multiple N‐terminal sites and target it for rapid degradation in response to genotoxic stress. Here we show that Chk1, but not Chk2, phosphory...

Full description

Saved in:
Bibliographic Details
Main Authors: Uto, Katsuhiro (Author) , Inoue, Daigo (Author)
Format: Article (Journal)
Language:English
Published: 22 July 2004
In: The EMBO journal
Year: 2004, Volume: 23, Issue: 16, Pages: 3386-3396
ISSN:1460-2075
DOI:10.1038/sj.emboj.7600328
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/sj.emboj.7600328
Verlag, kostenfrei, Volltext: http://emboj.embopress.org/content/23/16/3386
Get full text
Author Notes:Katsuhiro Uto, Daigo Inoue, Ken Shimuta, Nobushige Nakajo, Noriyuki Sagata

MARC

LEADER 00000caa a2200000 c 4500
001 1560111925
003 DE-627
005 20220813183749.0
007 cr uuu---uuuuu
008 170622s2004 xx |||||o 00| ||eng c
024 7 |a 10.1038/sj.emboj.7600328  |2 doi 
035 |a (DE-627)1560111925 
035 |a (DE-576)490111920 
035 |a (DE-599)BSZ490111920 
035 |a (OCoLC)1340975799 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Uto, Katsuhiro  |e VerfasserIn  |0 (DE-588)1135793042  |0 (DE-627)890873690  |0 (DE-576)490111343  |4 aut 
245 1 0 |a Chk1, but not Chk2, inhibits Cdc25 phosphatases by a novel common mechanism  |c Katsuhiro Uto, Daigo Inoue, Ken Shimuta, Nobushige Nakajo, Noriyuki Sagata 
264 1 |c 22 July 2004 
300 |a 11 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 22.06.2017 
520 |a Cdc25 phosphatases activate cyclin‐dependent kinases (Cdks) and thereby promote cell cycle progression. In vertebrates, Chk1 and Chk2 phosphorylate Cdc25A at multiple N‐terminal sites and target it for rapid degradation in response to genotoxic stress. Here we show that Chk1, but not Chk2, phosphorylates Xenopus Cdc25A at a novel C‐terminal site (Thr504) and inhibits it from C‐terminally interacting with various Cdk-cyclin complexes, including Cdk1-cyclin A, Cdk1-cyclin B, and Cdk2-cyclin E. Strikingly, this inhibition, rather than degradation itself, of Cdc25A is essential for the Chk1‐induced cell cycle arrest and the DNA replication checkpoint in early embryos. 14‐3‐3 proteins bind to Chk1‐phosphorylated Thr504, but this binding is not required for the inhibitory effect of Thr504 phosphorylation. A C‐terminal site presumably equivalent to Thr504 exists in all known Cdc25 family members from yeast to humans, and its phosphorylation by Chk1 (but not Chk2) can also inhibit all examined Cdc25 family members from C‐terminally interacting with their Cdk-cyclin substrates. Thus, Chk1 but not Chk2 seems to inhibit virtually all Cdc25 phosphatases by a novel common mechanism. 
650 4 |a Cdc25 
650 4 |a cell cycle checkpoint 
650 4 |a Chk1 
650 4 |a Chk2 
650 4 |a Xenopus 
700 1 |a Inoue, Daigo  |e VerfasserIn  |0 (DE-588)1078960194  |0 (DE-627)83996174X  |0 (DE-576)452098580  |4 aut 
773 0 8 |i Enthalten in  |a European Molecular Biology Organization  |t The EMBO journal  |d [London] : Nature Publishing Group UK, 1982  |g 23(2004), 16, Seite 3386-3396  |h Online-Ressource  |w (DE-627)266022529  |w (DE-600)1467419-1  |w (DE-576)075961377  |x 1460-2075  |7 nnas 
773 1 8 |g volume:23  |g year:2004  |g number:16  |g pages:3386-3396  |g extent:11  |a Chk1, but not Chk2, inhibits Cdc25 phosphatases by a novel common mechanism 
856 4 0 |u http://dx.doi.org/10.1038/sj.emboj.7600328  |x Verlag  |x Resolving-System  |z kostenfrei  |3 Volltext 
856 4 0 |u http://emboj.embopress.org/content/23/16/3386  |x Verlag  |z kostenfrei  |3 Volltext 
951 |a AR 
992 |a 20170622 
993 |a Article 
994 |a 2004 
998 |g 1078960194  |a Inoue, Daigo  |m 1078960194:Inoue, Daigo  |p 2 
999 |a KXP-PPN1560111925  |e 2972332644 
BIB |a Y 
SER |a journal 
JSO |a {"relHost":[{"title":[{"title":"The EMBO journal","title_sort":"EMBO journal"}],"pubHistory":["1.1982 -"],"part":{"extent":"11","volume":"23","text":"23(2004), 16, Seite 3386-3396","pages":"3386-3396","issue":"16","year":"2004"},"titleAlt":[{"title":"The EMBO journal online"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"European Molecular Biology OrganizationThe EMBO journal","note":["Gesehen am 18.02.2016","Fortsetzung der Druckausgabe"],"recId":"266022529","corporate":[{"role":"aut","roleDisplay":"VerfasserIn","display":"European Molecular Biology Organization"}],"language":["eng"],"origin":[{"dateIssuedKey":"1982","publisher":"Nature Publishing Group UK ; EMBO Press ; Oxford Univ. Press ; Nature Publ. Group ; Wiley","dateIssuedDisp":"1982-","publisherPlace":"[London] ; Heidelberg ; Oxford [u.a.] ; London [u.a.] ; Hoboken, NJ [u.a.]"}],"id":{"issn":["1460-2075"],"zdb":["1467419-1"],"eki":["266022529"],"doi":["10.1002/(ISSN)1460-2075"]},"name":{"displayForm":["publ. for the European Molecular Biology Organization"]},"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"11 S."}],"id":{"doi":["10.1038/sj.emboj.7600328"],"eki":["1560111925"]},"origin":[{"dateIssuedKey":"2004","dateIssuedDisp":"22 July 2004"}],"name":{"displayForm":["Katsuhiro Uto, Daigo Inoue, Ken Shimuta, Nobushige Nakajo, Noriyuki Sagata"]},"recId":"1560111925","language":["eng"],"note":["Gesehen am 22.06.2017"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title":"Chk1, but not Chk2, inhibits Cdc25 phosphatases by a novel common mechanism","title_sort":"Chk1, but not Chk2, inhibits Cdc25 phosphatases by a novel common mechanism"}],"person":[{"given":"Katsuhiro","family":"Uto","role":"aut","roleDisplay":"VerfasserIn","display":"Uto, Katsuhiro"},{"display":"Inoue, Daigo","roleDisplay":"VerfasserIn","role":"aut","family":"Inoue","given":"Daigo"}]} 
SRT |a UTOKATSUHICHK1BUTNOT2220 

Similar Items