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The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis

Vacuoles are multifunctional organelles essential for the sessile lifestyle of plants. Despite their central functions in cell growth, storage, and detoxification, knowledge about mechanisms underlying their biogenesis and associated protein trafficking pathways remains limited. Here, we show that i...

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Main Authors: Viotti, Corrado (Author) , Krüger, Falco (Author) , Krebs, Melanie (Author) , Neubert, Christoph (Author) , Fink, Fabian (Author) , Lupanga, Upendo (Author) , Scheuring, David (Author) , Hillmer, Stefan (Author) , Schumacher, Karin (Author)
Format: Article (Journal)
Language:English
Published: October 28, 2013
In: The plant cell
Year: 2013, Volume: 25, Issue: 9, Pages: 3434-3449
ISSN:1532-298X
DOI:10.1105/tpc.113.114827
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1105/tpc.113.114827
Verlag, kostenfrei, Volltext: http://www.plantcell.org/content/25/9/3434
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Author Notes:Corrado Viotti, Falco Krüger, Melanie Krebs, Christoph Neubert, Fabian Fink, Upendo Lupanga, David Scheuring, Yohann Boutté, Márcia Frescatada-Rosa, Susanne Wolfenstetter, Norbert Sauer, Stefan Hillmer, Markus Grebe and Karin Schumacher
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Summary:Vacuoles are multifunctional organelles essential for the sessile lifestyle of plants. Despite their central functions in cell growth, storage, and detoxification, knowledge about mechanisms underlying their biogenesis and associated protein trafficking pathways remains limited. Here, we show that in meristematic cells of the Arabidopsis thaliana root, biogenesis of vacuoles as well as the trafficking of sterols and of two major tonoplast proteins, the vacuolar H+-pyrophosphatase and the vacuolar H+-adenosinetriphosphatase, occurs independently of endoplasmic reticulum (ER)-Golgi and post-Golgi trafficking. Instead, both pumps are found in provacuoles that structurally resemble autophagosomes but are not formed by the core autophagy machinery. Taken together, our results suggest that vacuole biogenesis and trafficking of tonoplast proteins and lipids can occur directly from the ER independent of Golgi function.
Item Description:Gesehen am 06.07.2017
Physical Description:Online Resource
ISSN:1532-298X
DOI:10.1105/tpc.113.114827

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