Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity
Antibody therapy of solid cancers is well established, but suffers from unsatisfactory tumor penetration of large immunoglobulins or from low serum retention of antibody fragments. Oncolytic viruses are in advanced clinical development showing excellent safety, but suboptimal potency due to limited...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2015
|
| In: |
International journal of cancer
Year: 2015, Jahrgang: 136, Heft: 9, Pages: 2228-2240 |
| ISSN: | 1097-0215 |
| DOI: | 10.1002/ijc.29258 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1002/ijc.29258 Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/ijc.29258/abstract 
|
| Verfasserangaben: | Inés Fernández-Ulibarri, Katharina Hammer, Michaela A.E. Arndt, Johanna K. Kaufmann, Dominik Dorer, Sarah Engelhardt, Roland E. Kontermann, Jochen Hess, Heike Allgayer, Jürgen Krauss, Dirk M. Nettelbeck |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1560639598 | ||
| 003 | DE-627 | ||
| 005 | 20220813191921.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170707s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/ijc.29258 |2 doi | |
| 035 | |a (DE-627)1560639598 | ||
| 035 | |a (DE-576)490639593 | ||
| 035 | |a (DE-599)BSZ490639593 | ||
| 035 | |a (OCoLC)1340976844 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fernandez Ulibarri, Ines |e VerfasserIn |0 (DE-588)1136494782 |0 (DE-627)892993960 |0 (DE-576)490639321 |4 aut | |
| 245 | 1 | 0 | |a Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity |c Inés Fernández-Ulibarri, Katharina Hammer, Michaela A.E. Arndt, Johanna K. Kaufmann, Dominik Dorer, Sarah Engelhardt, Roland E. Kontermann, Jochen Hess, Heike Allgayer, Jürgen Krauss, Dirk M. Nettelbeck |
| 264 | 1 | |c 2015 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a First published: 12 November 2014 | ||
| 500 | |a Gesehen am 07.07.2017 | ||
| 520 | |a Antibody therapy of solid cancers is well established, but suffers from unsatisfactory tumor penetration of large immunoglobulins or from low serum retention of antibody fragments. Oncolytic viruses are in advanced clinical development showing excellent safety, but suboptimal potency due to limited virus spread within tumors. Here, by developing an immunoRNase-encoding oncolytic adenovirus, we combine viral oncolysis with intratumoral genetic delivery of a small antibody-fusion protein for targeted bystander killing of tumor cells (viro-antibody therapy). Specifically, we explore genetic delivery of a small immunoRNase consisting of an EGFR-binding scFv antibody fragment fused to the RNase Onconase (ONCEGFR) that induces tumor cell death by RNA degradation after cellular internalization. Onconase is a frog RNase that combines lack of immunogenicity and excellent safety in patients with high tumor killing potency due to its resistance to the human cytosolic RNase inhibitor. We show that ONCEGFR expression by oncolytic adenoviruses is feasible with an optimized, replication-dependent gene expression strategy. Virus-encoded ONCEGFR induces potent and EGFR-dependent bystander killing of tumor cells. Importantly, the ONCEGFR-encoding oncolytic adenovirus showed dramatically increased cytotoxicity specifically to EGFR-positive tumor cells in vitro and significantly enhanced therapeutic activity in a mouse xenograft tumor model. The latter demonstrates that ONCEGFR is expressed at levels sufficient to trigger tumor cell killing in vivo. The established ONCEGFR-encoding oncolytic adenovirus represents a novel agent for treatment of EGFR-positive tumors. This viro-antibody therapy platform can be further developed for targeted/personalized cancer therapy by exploiting antibody diversity to target further established or emerging tumor markers or combinations thereof. | ||
| 650 | 4 | |a EGFR | |
| 650 | 4 | |a immunoRNase | |
| 650 | 4 | |a oncolytic adenovirus | |
| 650 | 4 | |a Onconase | |
| 650 | 4 | |a scFv | |
| 700 | 1 | |a Arndt, Michaela |e VerfasserIn |0 (DE-588)1084612712 |0 (DE-627)848572076 |0 (DE-576)456369635 |4 aut | |
| 700 | 1 | |a Heß, Jochen |d 1971- |e VerfasserIn |0 (DE-588)121902390 |0 (DE-627)705696057 |0 (DE-576)292983417 |4 aut | |
| 700 | 1 | |a Allgayer, Heike |d 1969- |e VerfasserIn |0 (DE-588)1024987884 |0 (DE-627)720854466 |0 (DE-576)369805003 |4 aut | |
| 700 | 1 | |a Krauß, Jürgen |d 1960- |e VerfasserIn |0 (DE-588)120665301 |0 (DE-627)70490778X |0 (DE-576)292329083 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of cancer |d Bognor Regis : Wiley-Liss, 1966 |g 136(2015), 9, Seite 2228-2240 |h Online-Ressource |w (DE-627)269532781 |w (DE-600)1474822-8 |w (DE-576)079876129 |x 1097-0215 |7 nnas |a Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity |
| 773 | 1 | 8 | |g volume:136 |g year:2015 |g number:9 |g pages:2228-2240 |g extent:13 |a Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/ijc.29258 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://onlinelibrary.wiley.com/doi/10.1002/ijc.29258/abstract |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170707 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 120665301 |a Krauß, Jürgen |m 120665301:Krauß, Jürgen |d 910000 |e 910000PK120665301 |k 0/910000/ |p 10 | ||
| 998 | |g 1024987884 |a Allgayer, Heike |m 1024987884:Allgayer, Heike |d 60000 |d 61800 |e 60000PA1024987884 |e 61800PA1024987884 |k 0/60000/ |k 1/60000/61800/ |p 9 | ||
| 998 | |g 121902390 |a Heß, Jochen |m 121902390:Heß, Jochen |d 910000 |d 911000 |e 910000PH121902390 |e 911000PH121902390 |k 0/910000/ |k 1/910000/911000/ |p 8 | ||
| 998 | |g 1084612712 |a Arndt, Michaela |m 1084612712:Arndt, Michaela |d 910000 |e 910000PA1084612712 |k 0/910000/ |p 3 | ||
| 999 | |a KXP-PPN1560639598 |e 2973437946 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"family":"Fernandez Ulibarri","display":"Fernandez Ulibarri, Ines","given":"Ines","role":"aut"},{"family":"Arndt","given":"Michaela","display":"Arndt, Michaela","role":"aut"},{"given":"Jochen","display":"Heß, Jochen","role":"aut","family":"Heß"},{"family":"Allgayer","display":"Allgayer, Heike","given":"Heike","role":"aut"},{"family":"Krauß","role":"aut","display":"Krauß, Jürgen","given":"Jürgen"}],"title":[{"title":"Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity","title_sort":"Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity"}],"id":{"doi":["10.1002/ijc.29258"],"eki":["1560639598"]},"physDesc":[{"extent":"13 S."}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"name":{"displayForm":["Inés Fernández-Ulibarri, Katharina Hammer, Michaela A.E. Arndt, Johanna K. Kaufmann, Dominik Dorer, Sarah Engelhardt, Roland E. Kontermann, Jochen Hess, Heike Allgayer, Jürgen Krauss, Dirk M. Nettelbeck"]},"recId":"1560639598","relHost":[{"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activityInternational journal of cancer","physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"International journal of cancer","subtitle":"publication of the International Union against Cancer (UICC) = Journal international du cancer","title":"International journal of cancer"}],"part":{"extent":"13","volume":"136","issue":"9","pages":"2228-2240","year":"2015","text":"136(2015), 9, Seite 2228-2240"},"corporate":[{"role":"isb","display":"International Union against Cancer"}],"titleAlt":[{"title":"Predictive oncology"}],"language":["eng"],"titleTranslated":[{"translated":"Journal international du cancer"}],"id":{"zdb":["1474822-8"],"eki":["269532781"],"doi":["10.1002/(ISSN)1097-0215"],"issn":["1097-0215"]},"pubHistory":["1.1966 -"],"note":["Gesehen am 28.02.08","Darin: Predictive oncology"],"origin":[{"publisherPlace":"Bognor Regis","dateIssuedDisp":"1966-","publisher":"Wiley-Liss","dateIssuedKey":"1966"}],"recId":"269532781"}],"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"2015"}],"note":["First published: 12 November 2014","Gesehen am 07.07.2017"]} | ||
| SRT | |a FERNANDEZUGENETICDEL2015 | ||