Single-cell analysis uncovers clonal acinar cell heterogeneity in the adult pancreas
Acinar cells make up the majority of all cells in the pancreas, yet the source of new acinar cells during homeostasis remains unknown. Using multicolor lineage-tracing and organoid-formation assays, we identified the presence of a progenitor-like acinar cell subpopulation. These cells have long-term...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 October 2016
|
| In: |
Developmental cell
Year: 2016, Volume: 39, Issue: 3, Pages: 289-301 |
| ISSN: | 1878-1551 |
| DOI: | 10.1016/j.devcel.2016.10.002 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.devcel.2016.10.002 Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S1534580716307158 
|
| Author Notes: | Damian Wollny, Sheng Zhao, Isabelle Everlien, Xiaokang Lun, Jan Brunken, Daniel Brüne, Frederik Ziebell, Inna Tabansky, Wilko Weichert, Anna Marciniak-Czochra, Ana Martin-Villalba |
| Summary: | Acinar cells make up the majority of all cells in the pancreas, yet the source of new acinar cells during homeostasis remains unknown. Using multicolor lineage-tracing and organoid-formation assays, we identified the presence of a progenitor-like acinar cell subpopulation. These cells have long-term self-renewal capacity, albeit in a unipotent fashion. We further demonstrate that binuclear acinar cells are terminally differentiated acinar cells. Transcriptome analysis of single acinar cells revealed the existence of a minor population of cells expressing progenitor markers. Interestingly, a gain of the identified markers accompanied by a transient gain of proliferation was observed following chemically induced pancreatitis. Altogether, our study identifies a functionally and molecularly distinct acinar subpopulation and thus transforms our understanding of the acinar cell compartment as a pool of equipotent secretory cells. |
|---|---|
| Item Description: | Gesehen am 27.07.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1878-1551 |
| DOI: | 10.1016/j.devcel.2016.10.002 |