Efficient protein depletion by genetically controlled deprotection of a dormant N-degron: report
Methods that allow for the manipulation of genes or their products have been highly fruitful for biomedical research. Here, we describe a method that allows the control of protein abundance by a genetically encoded regulatory system. We developed a dormant N-degron that can be attached to the N-term...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2009 Apr 28
|
| In: |
Molecular systems biology
Year: 2009, Jahrgang: 5 |
| ISSN: | 1744-4292 |
| DOI: | 10.1038/msb.2009.25 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/msb.2009.25 Verlag, kostenfrei, Volltext: https://www.embopress.org/doi/full/10.1038/msb.2009.25 
|
| Verfasserangaben: | Christof Taxis, Gunter Stier, Roberta Spadaccini, and Michael Knop |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1562393006 | ||
| 003 | DE-627 | ||
| 005 | 20241015081233.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 170814s2009 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/msb.2009.25 |2 doi | |
| 035 | |a (DE-627)1562393006 | ||
| 035 | |a (DE-576)492393001 | ||
| 035 | |a (DE-599)BSZ492393001 | ||
| 035 | |a (OCoLC)1340978544 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Taxis, Christof |e VerfasserIn |0 (DE-588)1138290173 |0 (DE-627)895601729 |0 (DE-576)185600328 |4 aut | |
| 245 | 1 | 0 | |a Efficient protein depletion by genetically controlled deprotection of a dormant N-degron |b report |c Christof Taxis, Gunter Stier, Roberta Spadaccini, and Michael Knop |
| 264 | 1 | |c 2009 Apr 28 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 14.08.2017 | ||
| 520 | |a Methods that allow for the manipulation of genes or their products have been highly fruitful for biomedical research. Here, we describe a method that allows the control of protein abundance by a genetically encoded regulatory system. We developed a dormant N-degron that can be attached to the N-terminus of a protein of interest. Upon expression of a site-specific protease, the dormant N-degron becomes deprotected. The N-degron then targets itself and the attached protein for rapid proteasomal degradation through the N-end rule pathway. We use an optimized tobacco etch virus (TEV) protease variant combined with selective target binding to achieve complete and rapid deprotection of the N-degron-tagged proteins. This method, termed TEV protease induced protein inactivation (TIPI) of TIPI-degron (TDeg) modified target proteins is fast, reversible, and applicable to a broad range of proteins. TIPI of yeast proteins essential for vegetative growth causes phenotypes that are close to deletion mutants. The features of the TIPI system make it a versatile tool to study protein function in eukaryotes and to create new modules for synthetic or systems biology. | ||
| 700 | 1 | |a Stier, Gunter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Knop, Michael |e VerfasserIn |0 (DE-588)1064187552 |0 (DE-627)813076749 |0 (DE-576)423832107 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular systems biology |d [London] : Nature Publishing Group UK, 2005 |g 5(2009) Article number 267, 7 Seiten |h Online-Ressource |w (DE-627)490536905 |w (DE-600)2193510-5 |w (DE-576)28127942X |x 1744-4292 |7 nnas |a Efficient protein depletion by genetically controlled deprotection of a dormant N-degron report |
| 773 | 1 | 8 | |g volume:5 |g year:2009 |g extent:7 |a Efficient protein depletion by genetically controlled deprotection of a dormant N-degron report |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/msb.2009.25 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.embopress.org/doi/full/10.1038/msb.2009.25 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20170814 | ||
| 993 | |a Article | ||
| 994 | |a 2009 | ||
| 998 | |g 1064187552 |a Knop, Michael |m 1064187552:Knop, Michael |p 1 |x j | ||
| 999 | |a KXP-PPN1562393006 |e 2977699260 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"eki":["1562393006"],"doi":["10.1038/msb.2009.25"]},"relHost":[{"note":["Gesehen am 09.12.2025"],"origin":[{"publisherPlace":"[London] ; Heidelberg ; Hoboken, NJ [u.a.] ; London [u.a.]","publisher":"Nature Publishing Group UK ; EMBO Press ; Wiley ; Nature Publ. Group [u.a.]","dateIssuedDisp":"[2005]-"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title":"Molecular systems biology","title_sort":"Molecular systems biology"}],"pubHistory":["1.2005 -"],"language":["eng"],"recId":"490536905","disp":"Efficient protein depletion by genetically controlled deprotection of a dormant N-degron reportMolecular systems biology","physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"7","volume":"5","text":"5(2009) Article number 267, 7 Seiten","year":"2009"},"id":{"issn":["1744-4292"],"zdb":["2193510-5"],"doi":["10.1002/(ISSN)1744-4292"],"eki":["490536905"]}}],"person":[{"display":"Taxis, Christof","family":"Taxis","given":"Christof","role":"aut","roleDisplay":"VerfasserIn"},{"display":"Stier, Gunter","role":"aut","roleDisplay":"VerfasserIn","given":"Gunter","family":"Stier"},{"display":"Knop, Michael","roleDisplay":"VerfasserIn","role":"aut","family":"Knop","given":"Michael"}],"name":{"displayForm":["Christof Taxis, Gunter Stier, Roberta Spadaccini, and Michael Knop"]},"physDesc":[{"extent":"7 S."}],"note":["Gesehen am 14.08.2017"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedKey":"2009","dateIssuedDisp":"2009 Apr 28"}],"title":[{"title_sort":"Efficient protein depletion by genetically controlled deprotection of a dormant N-degron","title":"Efficient protein depletion by genetically controlled deprotection of a dormant N-degron","subtitle":"report"}],"recId":"1562393006","language":["eng"]} | ||
| SRT | |a TAXISCHRISEFFICIENTP2009 | ||