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Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation

Centromeres are the structural and functional foundation for kinetochore formation, spindle attachment, and chromosome segregation. In this study, we isolated factors required for centromere propagation using genome-wide RNA interference screening for defects in centromere protein A (CENP-A; centrom...

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Hauptverfasser: Erhardt, Sylvia (VerfasserIn) , Mellone, Barbara G. (VerfasserIn) , Betts, Craig M. (VerfasserIn) , Zhang, Weiguo (VerfasserIn) , Karpen, Gary H. (VerfasserIn) , Straight, Aaron F. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: December 1, 2008
In: The journal of cell biology
Year: 2008, Jahrgang: 183, Heft: 5, Pages: 805-818
ISSN:1540-8140
DOI:10.1083/jcb.200806038
Online-Zugang:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1083/jcb.200806038
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Verfasserangaben:Sylvia Erhardt, Barbara G. Mellone, Craig M. Betts, Weiguo Zhang, Gary H. Karpen, and Aaron F. Straight
Beschreibung
Zusammenfassung:Centromeres are the structural and functional foundation for kinetochore formation, spindle attachment, and chromosome segregation. In this study, we isolated factors required for centromere propagation using genome-wide RNA interference screening for defects in centromere protein A (CENP-A; centromere identifier [CID]) localization in Drosophila melanogaster. We identified the proteins CAL1 and CENP-C as essential factors for CID assembly at the centromere. CID, CAL1, and CENP-C coimmunoprecipitate and are mutually dependent for centromere localization and function. We also identified the mitotic cyclin A (CYCA) and the anaphase-promoting complex (APC) inhibitor RCA1/Emi1 as regulators of centromere propagation. We show that CYCA is centromere localized and that CYCA and RCA1/Emi1 couple centromere assembly to the cell cycle through regulation of the fizzy-related/CDH1 subunit of the APC. Our findings identify essential components of the epigenetic machinery that ensures proper specification and propagation of the centromere and suggest a mechanism for coordinating centromere inheritance with cell division.
Beschreibung:Gesehen am 15.08.2017
Beschreibung:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.200806038

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