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Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines

Epitope tagging of proteins as a strategy for the analysis of function, interactions and the subcellular distribution of proteins has become widely used. In the yeast Saccharomyces cerevisiae, molecular biological techniques have been developed that use a simple PCR-based strategy to introduce epito...

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Bibliographic Details
Main Authors: Knop, Michael (Author) , Pereira, Gislene (Author) , Zachariae, Wolfgang (Author) , Nasmyth, Kim (Author) , Schiebel, Elmar (Author)
Format: Article (Journal)
Language:English
Published: 16 July 1999
In: Yeast
Year: 1999, Volume: 15, Issue: 10B, Pages: 963-972
ISSN:1097-0061
DOI:10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W
Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W/abstract
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Author Notes:Michael Knop, Katja Siegers, Gislene Pereira, Wolfgang Zachariae, Barbara Winsor, Kim Nasmyth and Elmar Schiebel

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