The spindle pole body component Spc98p interacts with the γ‐tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment
Tub4p is a novel tubulin found in Saccharomyces cerevisiae. It most resembles gamma-tubulin and, like it, is localized to the yeast microtubule organizing centre, the spindle pole body (SPB). In this paper we report the identification of SPC98 as a dosage-dependent suppressor of the conditional leth...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1996 Aug 1
|
| In: |
The EMBO journal
Year: 1996, Jahrgang: 15, Heft: 15, Pages: 3899-3911 |
| ISSN: | 1460-2075 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC452092/
|
| Verfasserangaben: | Silke Geissler, Gislene Pereira, Anne Spang, Michael Knop, Sylvie Souès, John Kilmartin and Elmar Schiebel |
| Zusammenfassung: | Tub4p is a novel tubulin found in Saccharomyces cerevisiae. It most resembles gamma-tubulin and, like it, is localized to the yeast microtubule organizing centre, the spindle pole body (SPB). In this paper we report the identification of SPC98 as a dosage-dependent suppressor of the conditional lethal tub4-1 allele. SPC98 encodes an SPB component of 98 kDa which is identical to the previously described 90 kDa SPB protein. Strong overexpression of SPC98 is toxic, causing cells to arrest with a large bud, defective microtubule structures, undivided nucleus and replicated DNA. The toxicity of SPC98 overexpression was relieved by co-overexpression of TUB4. Further evidence for an interaction between Tub4p and Spc98p came from the synthetic toxicity of tub4-1 and spc98-1 alleles, the dosage-dependent suppression of spc98-4 by TUB4, the binding of Tub4p to Spc98p in the two-hybrid system and the co-immunoprecipitation of Tub4p and Spc98p. In addition, Spc98-1p is defective in its interaction with Tub4p in the two-hybrid system. We suggest a model in which Tub4p and Spc98p form a complex involved in microtubule organization by the SPB. |
|---|---|
| Beschreibung: | Gesehen am 31.08.2017 |
| Beschreibung: | Online Resource |
| ISSN: | 1460-2075 |