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PIEZO2 is required for mechanotransduction in human stem cell-derived touch receptors

Human sensory neurons are inaccessible for functional examination, and thus little is known about the mechanisms mediating touch sensation in humans. Here we demonstrate that the mechanosensitivity of human embryonic stem (hES) cell-derived touch receptors depends on PIEZO2. To recapitulate sensory...

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Hauptverfasser: Schrenk-Siemens, Katrin (VerfasserIn) , Wende, Hagen (VerfasserIn) , Prato, Vincenzo (VerfasserIn) , Song, Kun (VerfasserIn) , Rostock, Charlotte (VerfasserIn) , Utikal, Jochen (VerfasserIn) , Lechner, Stefan (VerfasserIn) , Siemens, Jan (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2015
In: Nature neuroscience
Year: 2015, Jahrgang: 18, Heft: 1, Pages: 10-16
ISSN:1546-1726
DOI:10.1038/nn.3894
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1038/nn.3894
Verlag, Volltext: http://www.nature.com.ezproxy.medma.uni-heidelberg.de/neuro/journal/v18/n1/full/nn.3894.html?foxtrotcallback=true
Volltext
Verfasserangaben:Katrin Schrenk-Siemens, Hagen Wende, Vincenzo Prato, Kun Song, Charlotte Rostock, Alexander Loewer, Jochen Utikal, Gary R. Lewin, Stefan G. Lechner and Jan Siemens
Beschreibung
Zusammenfassung:Human sensory neurons are inaccessible for functional examination, and thus little is known about the mechanisms mediating touch sensation in humans. Here we demonstrate that the mechanosensitivity of human embryonic stem (hES) cell-derived touch receptors depends on PIEZO2. To recapitulate sensory neuron development in vitro, we established a multistep differentiation protocol and generated sensory neurons via the intermediate production of neural crest cells derived from hES cells or human induced pluripotent stem (hiPS) cells. The generated neurons express a distinct set of touch receptor-specific genes and convert mechanical stimuli into electrical signals, their most salient characteristic in vivo. Strikingly, mechanosensitivity is lost after CRISPR/Cas9-mediated PIEZO2 gene deletion. Our work establishes a model system that resembles human touch receptors, which may facilitate mechanistic analysis of other sensory subtypes and provide insight into developmental programs underlying sensory neuron diversity.
Beschreibung:Published: 3 December 2014
Gesehen am 06.09.2017
Beschreibung:Online Resource
ISSN:1546-1726
DOI:10.1038/nn.3894

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