Buchumschlag

Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract

The enteric nervous system (ENS) orchestrates a broad range of important gastrointestinal functions such as intestinal motility and gastric secretion. The ENS can be affected by environmental factors, diet and disease. Changes due to these alterations are often hard to evaluate in detail when whole...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Grundmann, David (VerfasserIn) , Schäfer, Karl Herbert (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 20 March 2015
In: Scientific reports
Year: 2015, Jahrgang: 5
ISSN:2045-2322
DOI:10.1038/srep09226
Online-Zugang:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/srep09226
Verlag, kostenfrei, Volltext: https://www-nature-com.ezproxy.medma.uni-heidelberg.de/articles/srep09226
Volltext
Verfasserangaben:David Grundmann, Markus Klotz, Holger Rabe, Matthias Glanemann and Karl-Herbert Schäfer

MARC

LEADER 00000caa a2200000 c 4500
001 1564043924
003 DE-627
005 20230427142045.0
007 cr uuu---uuuuu
008 171004s2015 xx |||||o 00| ||eng c
024 7 |a 10.1038/srep09226  |2 doi 
035 |a (DE-627)1564043924 
035 |a (DE-576)49404392X 
035 |a (DE-599)BSZ49404392X 
035 |a (OCoLC)1340979943 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Grundmann, David  |d 1979-  |e VerfasserIn  |0 (DE-588)113138329X  |0 (DE-627)886042488  |0 (DE-576)488254647  |4 aut 
245 1 0 |a Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract  |c David Grundmann, Markus Klotz, Holger Rabe, Matthias Glanemann and Karl-Herbert Schäfer 
264 1 |c 20 March 2015 
300 |a 8 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 04.10.2017 
520 |a The enteric nervous system (ENS) orchestrates a broad range of important gastrointestinal functions such as intestinal motility and gastric secretion. The ENS can be affected by environmental factors, diet and disease. Changes due to these alterations are often hard to evaluate in detail when whole gut samples are used. Analyses based on pure ENS tissue can more effectively reflect the ongoing changes during pathological processes. Here, we present an optimized approach for the isolation of pure myenteric plexus (MP) from adult mouse and human. To do so, muscle tissue was individually digested with a purified collagenase. After incubation and a gentle mechanical disruption step, MP networks could be collected with anatomical integrity. These tissues could be stored and used either for immediate genomic, proteomic or in vitro approaches, and enteric neurospheres could be generated and differentiated. In a pilot experiment, the influence of bacterial lipopolysaccharide on human MP was analyzed using 2-dimensional gel electrophoresis. The method also allows investigation of factors that are secreted by myenteric tissue in vitro. The isolation of pure MP in large amounts allows new analytical approaches that can provide a new perspective in evaluating changes of the ENS in experimental models, human disease and aging. 
700 1 |a Schäfer, Karl Herbert  |d 1957-  |e VerfasserIn  |0 (DE-588)172647320  |0 (DE-627)697581144  |0 (DE-576)13350607X  |4 aut 
773 0 8 |i Enthalten in  |t Scientific reports  |d [London] : Springer Nature, 2011  |g 5(2015) Artikel-Nummer 09226, 8 Seiten  |h Online-Ressource  |w (DE-627)663366712  |w (DE-600)2615211-3  |w (DE-576)346641179  |x 2045-2322  |7 nnas  |a Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract 
773 1 8 |g volume:5  |g year:2015  |g extent:8  |a Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract 
856 4 0 |u http://dx.doi.org/10.1038/srep09226  |x Verlag  |x Resolving-System  |z kostenfrei  |3 Volltext 
856 4 0 |u https://www-nature-com.ezproxy.medma.uni-heidelberg.de/articles/srep09226  |x Verlag  |z kostenfrei  |3 Volltext 
951 |a AR 
992 |a 20171004 
993 |a Article 
994 |a 2015 
998 |g 172647320  |a Schäfer, Karl Herbert  |m 172647320:Schäfer, Karl Herbert  |d 60000  |d 62200  |e 60000PS172647320  |e 62200PS172647320  |k 0/60000/  |k 1/60000/62200/  |p 5  |y j 
999 |a KXP-PPN1564043924  |e 298267307X 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"roleDisplay":"VerfasserIn","display":"Grundmann, David","role":"aut","family":"Grundmann","given":"David"},{"given":"Karl Herbert","family":"Schäfer","role":"aut","roleDisplay":"VerfasserIn","display":"Schäfer, Karl Herbert"}],"title":[{"title":"Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract","title_sort":"Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract"}],"recId":"1564043924","language":["eng"],"note":["Gesehen am 04.10.2017"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"name":{"displayForm":["David Grundmann, Markus Klotz, Holger Rabe, Matthias Glanemann and Karl-Herbert Schäfer"]},"id":{"eki":["1564043924"],"doi":["10.1038/srep09226"]},"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"20 March 2015"}],"relHost":[{"origin":[{"dateIssuedKey":"2011","publisher":"Springer Nature ; Nature Publishing Group","dateIssuedDisp":"2011-","publisherPlace":"[London] ; London"}],"id":{"issn":["2045-2322"],"zdb":["2615211-3"],"eki":["663366712"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Scientific reports","title_sort":"Scientific reports"}],"pubHistory":["1, article number 1 (2011)-"],"part":{"year":"2015","extent":"8","text":"5(2015) Artikel-Nummer 09226, 8 Seiten","volume":"5"},"note":["Gesehen am 12.07.24"],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tractScientific reports","recId":"663366712","language":["eng"]}],"physDesc":[{"extent":"8 S."}]} 
SRT |a GRUNDMANNDISOLATIONO2020 

Ähnliche Einträge