Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry
Lupinus albus cell cultures secrete a large set of hydrolases into their medium with a small number of highly abundant proteins. We have investigated the protein composition of the medium with two different methods, two-dimensional gel electrophoresis-electrospray ionisation tandem mass spectrometry...
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| Hauptverfasser: | , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2003
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| In: |
Journal of chromatography
Year: 2003, Jahrgang: 1009, Heft: 1, Pages: 105-110 |
| ISSN: | 1873-3778 |
| DOI: | 10.1016/S0021-9673(03)00622-8 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/S0021-9673(03)00622-8
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| Verfasserangaben: | Thomas Schwend, Inga Redwanz, Thomas Ruppert, Andrea Szenthe, Michael Wink |
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| 520 | |a Lupinus albus cell cultures secrete a large set of hydrolases into their medium with a small number of highly abundant proteins. We have investigated the protein composition of the medium with two different methods, two-dimensional gel electrophoresis-electrospray ionisation tandem mass spectrometry (ESI-MS-MS) and enzymatic analysis. The proteomic approach revealed the presence of several abundant proteins that had been overlooked using standard enzyme assays, e.g. subtilisin-like protease, glucan 1,3-β-glucosidase, α-amylase, chitinase, thaumatin-like protein, and a secretory pathogenesis-related protein. Several low-abundant proteins were readily detectable by enzymatic assays (peroxidases, phosphatase), but could not be found by ESI-MS-MS. Both data sets support the assumed lytic function of the medium, which appears to be similar to that of the plant vacuole. | ||
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