Differential uptake mechanisms of fluorescent substrates into stem-cell-derived serotonergic neurons
The actions of the neurotransmitters serotonin, dopamine, and norepinephrine are partly terminated by diffusion and in part by their uptake into neurons via the selective, high-affinity transporters for serotonin (SERT), dopamine (DAT), and norepinephrine (NET), respectively. There is also growing e...
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| Main Authors: | , , |
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| Format: | Article (Journal) Editorial |
| Language: | English |
| Published: |
October 26, 2015
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| In: |
ACS chemical neuroscience
Year: 2015, Volume: 6, Issue: 12, Pages: 1906-1912 |
| ISSN: | 1948-7193 |
| DOI: | 10.1021/acschemneuro.5b00219 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1021/acschemneuro.5b00219
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| Author Notes: | Friederike Matthaeus, Patrick Schloss, and Thorsten Lau |
| Summary: | The actions of the neurotransmitters serotonin, dopamine, and norepinephrine are partly terminated by diffusion and in part by their uptake into neurons via the selective, high-affinity transporters for serotonin (SERT), dopamine (DAT), and norepinephrine (NET), respectively. There is also growing evidence that all three monoamines are taken up into neurons by low-affinity, high-capacity organic cation transporters (OCT) and the plasma membrane monoamine transporter (PMAT). Pharmacological characterization of these low-affinity recombinant transporter proteins in heterologous expression systems has revealed that they are not antagonized by classical inhibitors of SERT, DAT, or NET but that decynium-22 (D22) antagonizes OCT3 and PMAT, whereas corticosterone and progesterone selectively inhibit OCT3. Here, we show that SERT, PMAT, and OCT3, but not OCT1 and OCT2, are coexpressed in murine stem cell-derived serotonergic neurons. Using selective antagonists, we provide evidence that uptake of the fluorescent substrates FFN511, ASP+, and 5-HT into stem cell-derived serotonergic neurons is mediated differentially by these transporters and also involves an as yet unknown transport mechanism. |
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| Item Description: | Gesehen am 17.10.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1948-7193 |
| DOI: | 10.1021/acschemneuro.5b00219 |