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Increased cyclooxygenase-2 expression in peripheral blood mononuclear cells of smokers and hyperlipidemic subjects

Cyclooxygenase (COX)-2 is expressed in macrophages of arteriosclerotic lesions and promotes inflammation. We investigated whether COX-2 is already expressed in peripheral blood mononuclear cells (PBMCs) of subjects possessing risk-related factors, such as in smokers and hyperlipidemics. PBMCs were i...

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Hauptverfasser: Bonaterra, Gabriel (VerfasserIn) , Hildebrandt, Wulf (VerfasserIn) , Bodens, Anne (VerfasserIn) , Sauer, Roland (VerfasserIn) , Dugi, Klaus A. (VerfasserIn) , Deigner, Hans-Peter (VerfasserIn) , Dröge, Wulf (VerfasserIn) , Metz, Jürgen (VerfasserIn) , Kinscherf, Ralf (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2005
In: Free radical biology and medicine
Year: 2004, Jahrgang: 38, Heft: 2, Pages: 235-242
ISSN:1873-4596
DOI:10.1016/j.freeradbiomed.2004.10.021
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1016/j.freeradbiomed.2004.10.021
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0891584904008433
Volltext
Verfasserangaben:Gabriel A. Bonaterra, Wulf Hildebrandt, Anne Bodens, Roland Sauer, Klaus A. Dugi, Hans-Peter Deigner, Wulf Dröge, Jürgen Metz, Ralf Kinscherf
Beschreibung
Zusammenfassung:Cyclooxygenase (COX)-2 is expressed in macrophages of arteriosclerotic lesions and promotes inflammation. We investigated whether COX-2 is already expressed in peripheral blood mononuclear cells (PBMCs) of subjects possessing risk-related factors, such as in smokers and hyperlipidemics. PBMCs were isolated from the venous blood of normolipidemic nonsmokers (NL-NSM; n = 15), normolipidemic smokers (NL-SM; n = 12), hyperlipidemic nonsmokers (HL-NSM; n = 10), and hyperlipidemic smokers (HL-SM; n = 10). RNA from PBMCs was used for RT-PCR. Plasma concentrations of oxidized low-density lipoproteins (oxLDL) were measured by ELISA, those of glutamate and cystine by HPLC. The results show that COX-2 expression in PBMCs was significantly increased in the groups with cardiovascular risk factors (NL-SM, HL-SM, HL-NSM) compared with NL-NSM. COX-2 expression in PBMCs was positively correlated with concentrations of total serum cholesterol, oxLDL, glutamate, or cystine. We suggest that the elevated COX-2 expression indicates a priming of PBMCs as a response to a systemic pro-oxidative and proinflammatory shift in subjects with cardiovascular risk factors, which might also contribute to growth and instability of arteriosclerotic lesions.
Beschreibung:Available online: 11 November 2004
Gesehen am 23.10.2017
Beschreibung:Online Resource
ISSN:1873-4596
DOI:10.1016/j.freeradbiomed.2004.10.021

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