Identification of breast cancer associated altered DNA methylation in peripheral blood using MALDI-TOF mass spectrometry
© The Author 2016. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: journals.permissionsoup.com.Background: Breast cancer (BC) is the leading cause of cancer-related mortality in women worldwide. Changes...
Gespeichert in:
| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
11 October 2016
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| In: |
Annals of oncology
Year: 2016, Jahrgang: 27 |
| ISSN: | 1569-8041 |
| DOI: | 10.1093/annonc/mdw363.54 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1093/annonc/mdw363.54 Verlag, Volltext: https://academic.oup.com/annonc/article/27/suppl_6/106P/2798832 
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| Verfasserangaben: | R. Yang, B. Burwinkel |
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| 520 | |a © The Author 2016. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: journals.permissionsoup.com.Background: Breast cancer (BC) is the leading cause of cancer-related mortality in women worldwide. Changes in DNA methylation in peripheral blood could be associated with malignancy at early stage. However, the BC-associated DNA methylation signatures in peripheral blood were largely unknown.Methods: Illumina 27K Methylation Array and Illumina 450K Methylation Array for the discovery of BC-related aberrant methylation sites in peripheral blood. The top hints were selected and validated using the MALDI-TOF Mass Spectrometry (MassARRAY, Agena Bioscience, Inc.) in two independent case-control studies with subjects from different centers. Gene expression levels were measured by real-time PCR and correlated with methylation. The clustering of samples by multiple CpG sites from a total of eight genes was realized by logistic regression. Receiver operating characteristic curve analyses... | ||
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