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Turnover of acetylcholine receptors at the endplate revisited: novel insights into nerve-dependent behavior

The turnover of nicotinic acetylcholine receptors (AChR) is a critical factor that determines function and safety of neuromuscular transmission at the nerve-muscle synapses, i.e. neuromuscular junctions (NMJs). Previously, three different populations of AChRs exhibiting distinct stereotypic and acti...

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Bibliographische Detailangaben
Hauptverfasser: Strack, Siegfried (VerfasserIn) , Khan, Muzamil Majid (VerfasserIn) , Rudolf, Rüdiger (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 15 August 2015
In: Journal of muscle research and cell motility
Year: 2015, Jahrgang: 36, Heft: 6, Pages: 517-524
ISSN:1573-2657
DOI:10.1007/s10974-015-9418-0
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1007/s10974-015-9418-0
Verlag, Volltext: https://link.springer.com/article/10.1007/s10974-015-9418-0
Volltext
Verfasserangaben:Siegfried Strack, Muzamil Majid Khan, Franziska Wild, Anika Rall, Rüdiger Rudolf
Beschreibung
Zusammenfassung:The turnover of nicotinic acetylcholine receptors (AChR) is a critical factor that determines function and safety of neuromuscular transmission at the nerve-muscle synapses, i.e. neuromuscular junctions (NMJs). Previously, three different populations of AChRs exhibiting distinct stereotypic and activity-dependent half-life values were observed in mouse muscles. To address AChR turnover in more detail, we here employed a recently developed longitudinal radioiodine assay that is based on repetitive measurements of radio emission from the same animals over long periods of time in combination with systematic variation of the time elapsed between AChR pulse-labeling and muscle denervation. Modeling of the data revealed profiles of AChR de novo synthesis and receptor incorporation into the postsynaptic membrane. Furthermore, decay of pre-existing AChRs upon denervation showed a peculiar pattern corroborating earlier findings of a two-step stabilization of AChRs.
Beschreibung:Gesehen am 06.12.2017
Beschreibung:Online Resource
ISSN:1573-2657
DOI:10.1007/s10974-015-9418-0

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