Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors
Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cel...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
11 February 2010
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| In: |
Biophysical journal
Year: 2010, Jahrgang: 98, Heft: 4, Pages: 543-551 |
| ISSN: | 1542-0086 |
| DOI: | 10.1016/j.bpj.2009.11.001 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.bpj.2009.11.001 Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S0006349509016890 
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| Verfasserangaben: | C. Selhuber-Unkel, T. Erdmann, M. López-García, H. Kessler, U.S. Schwarz, and J.P. Spatz |
| Zusammenfassung: | Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spacings ≥90 nm, focal contact formation was inhibited and the detachment forces as well as the stiffness of the cell body were significantly decreased compared to spacings ≤50 nm. Analyzing cell detachment at the subcellular level revealed that rupture forces of focal contacts increase with loading rate as predicted by a theoretical model for adhesion clusters. Furthermore, we show that the weak link between the intra- and extracellular space is at the intracellular side of a focal contact. Our results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength. |
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| Beschreibung: | Gesehen am 08.12.2017 |
| Beschreibung: | Online Resource |
| ISSN: | 1542-0086 |
| DOI: | 10.1016/j.bpj.2009.11.001 |