Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality
Many diagnostic and therapeutic concepts require antibodies of high specificity. Recombinant binder libraries and related selection approaches allow the efficient isolation of antibodies against almost every target of interest. Nevertheless, it cannot be guaranteed that selected antibodies perform w...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2016
|
| In: |
New biotechnology
Year: 2016, Jahrgang: 33, Heft: 5, Part A, Pages: 574-581 |
| ISSN: | 1876-4347 |
| DOI: | 10.1016/j.nbt.2015.12.003 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.nbt.2015.12.003 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S187167841500271X 
|
| Verfasserangaben: | Janek Kibat, Thomas Schirrmann, Matthias J. Knape, Saskia Helmsing, Doris Meier, Michael Hust, Christoph Schröder, Daniela Bertinetti, Gerhard Winter, Khalid Pardes, Mia Funk, Andrea Vala, Nathalia Giese, Friedrich W. Herberg, Stefan Dübel and Jörg D. Hoheisel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1566441153 | ||
| 003 | DE-627 | ||
| 005 | 20230426064432.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 171215s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.nbt.2015.12.003 |2 doi | |
| 035 | |a (DE-627)1566441153 | ||
| 035 | |a (DE-576)496441159 | ||
| 035 | |a (DE-599)BSZ496441159 | ||
| 035 | |a (OCoLC)1340983640 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Kibat, Janek |e VerfasserIn |0 (DE-588)1148792279 |0 (DE-627)1009195492 |0 (DE-576)49644073X |4 aut | |
| 245 | 1 | 0 | |a Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality |c Janek Kibat, Thomas Schirrmann, Matthias J. Knape, Saskia Helmsing, Doris Meier, Michael Hust, Christoph Schröder, Daniela Bertinetti, Gerhard Winter, Khalid Pardes, Mia Funk, Andrea Vala, Nathalia Giese, Friedrich W. Herberg, Stefan Dübel and Jörg D. Hoheisel |
| 264 | 1 | |c 2016 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 17 December 2015 | ||
| 500 | |a Gesehen am 15.12.2017 | ||
| 520 | |a Many diagnostic and therapeutic concepts require antibodies of high specificity. Recombinant binder libraries and related selection approaches allow the efficient isolation of antibodies against almost every target of interest. Nevertheless, it cannot be guaranteed that selected antibodies perform well and interact specifically enough with analytes unless an elaborate characterisation is performed. Here, we present an approach to shorten this process by combining the selection of suitable antibodies with the identification of informative target molecules by means of antibody microarrays, thereby reducing the effort of antibody characterisation by concentrating on relevant molecules. In a pilot scheme, a library of 456 single-chain variable fragment (scFv) binders to 134 antigens was used. They were arranged in a microarray format and incubated with the protein content of clinical tissue samples isolated from pancreatic ductal adenocarcinoma and healthy pancreas, as well as recurrent and non-recurrent bladder tumours. We observed significant variation in the expression of the E3 ubiquitin-protein ligase (CHFR) as well as the glutamate receptor interacting protein 2 (GRIP2), for example, always with more than one of the scFvs binding to these targets. Only the relevant antibodies were then characterised further on antigen microarrays and by surface plasmon resonance experiments so as to select the most specific and highest affinity antibodies. These binders were in turn used to confirm a microarray result by immunohistochemistry analysis. | ||
| 700 | 1 | |a Giese, Nathalia |e VerfasserIn |0 (DE-588)1033180955 |0 (DE-627)740120018 |0 (DE-576)380769123 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t New biotechnology |d New York, NY [u.a.] : Elsevier, 2008 |g 33(2016), 5, Part A, Seite 574-581 |h Online-Ressource |w (DE-627)55568895X |w (DE-600)2400836-9 |w (DE-576)281507724 |x 1876-4347 |7 nnas |a Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality |
| 773 | 1 | 8 | |g volume:33 |g year:2016 |g number:5, Part A |g pages:574-581 |g extent:8 |a Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.nbt.2015.12.003 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S187167841500271X |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20171215 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 1033180955 |a Giese, Nathalia |m 1033180955:Giese, Nathalia |d 910000 |d 910200 |e 910000PG1033180955 |e 910200PG1033180955 |k 0/910000/ |k 1/910000/910200/ |p 13 | ||
| 999 | |a KXP-PPN1566441153 |e 2990080937 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"given":"Janek","family":"Kibat","role":"aut","display":"Kibat, Janek"},{"display":"Giese, Nathalia","family":"Giese","role":"aut","given":"Nathalia"}],"note":["Available online 17 December 2015","Gesehen am 15.12.2017"],"language":["eng"],"name":{"displayForm":["Janek Kibat, Thomas Schirrmann, Matthias J. Knape, Saskia Helmsing, Doris Meier, Michael Hust, Christoph Schröder, Daniela Bertinetti, Gerhard Winter, Khalid Pardes, Mia Funk, Andrea Vala, Nathalia Giese, Friedrich W. Herberg, Stefan Dübel and Jörg D. Hoheisel"]},"origin":[{"dateIssuedDisp":"2016","dateIssuedKey":"2016"}],"recId":"1566441153","id":{"doi":["10.1016/j.nbt.2015.12.003"],"eki":["1566441153"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"8 S."}],"title":[{"title_sort":"Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality","title":"Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality"}],"relHost":[{"part":{"volume":"33","text":"33(2016), 5, Part A, Seite 574-581","issue":"5, Part A","year":"2016","pages":"574-581","extent":"8"},"title":[{"title":"New biotechnology","title_sort":"New biotechnology"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisher":"Elsevier","dateIssuedKey":"2008","publisherPlace":"New York, NY [u.a.]","dateIssuedDisp":"2008-"}],"disp":"Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown qualityNew biotechnology","type":{"bibl":"periodical","media":"Online-Ressource"},"id":{"issn":["1876-4347"],"zdb":["2400836-9"],"eki":["55568895X"]},"recId":"55568895X","language":["eng"],"pubHistory":["25.2008 -"],"note":["Gesehen am 18.01.2022","Fortsetzung der Druck-Ausgabe"]}]} | ||
| SRT | |a KIBATJANEKUTILISATIO2016 | ||